Product Info Summary
SKU: | M00057-1 |
---|---|
Size: | 100ug/vial |
Reactive Species: | Human |
Host: | Mouse |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) ESR1 Monoclonal Antibody
View all ER alpha/NR3A1 Antibodies
SKU/Catalog Number
M00057-1
Size
100ug/vial
Form
Liquid
Description
Boster Bio Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) ESR1 Monoclonal Antibody (Catalog # M00057-1). Tested in Flow Cytometry, WB, IHC applications. This antibody reacts with Human.
Storage & Handling
Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
Cite This Product
Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) ESR1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00057-1)
Host
Mouse
Contents
Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Clonality
Monoclonal
Clone Number
Clone: ER506
Isotype
IgG1, kappa
Immunogen
Recombinant fragment (around aa 2-185) of human Estrogen Receptor alpha protein (exact sequence is proprietary)
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00057-1 is reactive to ESR1 in Human
Applications
M00057-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
66.216kDa
Background of ER alpha/NR3A1
This monoclonal antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
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Assay dilution & Images
Reconsitution
Restore with deionized water (or equivalent) for reconstitution volume of 100 µL
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.
Validation Images & Assay Conditions
Click image to see more details
Flow Cytometry for human ER-alpha on MCF-7 cells. Black: cells alone; Green: Isotype Control; Red: PE-labeled Estrogen Receptor alpha Mouse Monoclonal Antibody (ER506).
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Western Blot analysis of human MCF-7 cell lysate using Anti-Estrogen Receptor alpha Mouse Monoclonal Antibody (ER506).
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Formalin-fixed, paraffin-embedded human Endometrium stained with Anti-Estrogen Receptor alpha Mouse Monoclonal Antibody (ER506).
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SDS-PAGE analysis purified Anti-Estrogen Receptor alpha Mouse Monoclonal Antibody (ER506). Confirmation of integrity and purity of antibody.
Protein Target Info & Infographic
Gene/Protein Information For ESR1 (Source: Uniprot.org, NCBI)
Gene Name
ESR1
Full Name
Estrogen receptor
Weight
66.216kDa
Superfamily
nuclear hormone receptor family
Alternative Names
ER alpha; ER; Era; ER-alpha; ESR1; ESRESRA; Estradiol receptor; estrogen receptor 1; estrogen receptor alpha delta 3*4,56,7*/819-2 isoform; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha delta 4*5,6,7*/654 isoform; estrogen receptor alpha; estrogen receptor; NR3A1; NR3A1DKFZp686N23123; Nuclear receptor subfamily 3 group A member 1 ESR1 ER, ESR, ESRA, ESTRR, Era, NR3A1 estrogen receptor 1 estrogen receptor|E2 receptor alpha|ER-alpha|estradiol receptor|estrogen nuclear receptor alpha|estrogen receptor alpha E1-E2-1-2|estrogen receptor alpha E1-N2-E2-1-2|nuclear receptor subfamily 3 group A member 1|oestrogen receptor alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ESR1, check out the ESR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ESR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) ESR1 Monoclonal Antibody (M00057-1)
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No publications found for M00057-1
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) ESR1 Monoclonal Antibody
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary gland using anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2020-03-16
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-03-16
Question
Is a blocking peptide available for product anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody (M00057-1)?
Verified Customer
Verified customer
Asked: 2020-01-09
Answer
We do provide the blocking peptide for product anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody (M00057-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-09
Question
I was wanting to use your anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody for Flow Cytometry for human mammary gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human mammary gland identification?
Verified Customer
Verified customer
Asked: 2019-10-14
Answer
As indicated on the product datasheet, M00057-1 anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human mammary gland in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-14
Question
Does M00057-1 anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Brown
Verified customer
Asked: 2018-09-26
Answer
As indicated on the product datasheet, M00057-1 anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-26
Question
Does anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1 work on canine Flow Cytometry with endometrium?
Verified Customer
Verified customer
Asked: 2018-06-29
Answer
Our lab technicians have not validated anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine endometrium in Flow Cytometry, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-06-29
Question
Is there a BSA free version of anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1 available?
Verified Customer
Verified customer
Asked: 2018-03-07
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-03-07
Question
I see that the anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Z. Jones
Verified customer
Asked: 2015-10-26
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2015-10-26