Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) ESR1 Monoclonal Antibody

ER alpha/NR3A1 antibody

Boster Bio Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) ESR1 Monoclonal Antibody (Catalog # M00057-1). Tested in Flow Cytometry, WB, IHC applications. This antibody reacts with Human.

Product Info Summary

SKU: M00057-1
Size: 100ug/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IHC, WB

Product Name

Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) ESR1 Monoclonal Antibody

View all ER alpha/NR3A1 Antibodies

SKU/Catalog Number

M00057-1

Size

100ug/vial

Form

Liquid

Description

Boster Bio Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) ESR1 Monoclonal Antibody (Catalog # M00057-1). Tested in Flow Cytometry, WB, IHC applications. This antibody reacts with Human.

Storage & Handling

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Cite This Product

Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) ESR1 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00057-1)

Host

Mouse

Contents

Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Clonality

Monoclonal

Clone Number

Clone: ER506

Isotype

IgG1, kappa

Immunogen

Recombinant fragment (around aa 2-185) of human Estrogen Receptor alpha protein (exact sequence is proprietary)

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00057-1 is reactive to ESR1 in Human

Applications

M00057-1 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

66.216kDa

Background of ER alpha/NR3A1

This monoclonal antibody is specific to ER alpha and shows minimal cross-reaction with other members of the family. ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy. It strongly stains nuclei of epithelial cells in breast carcinomas.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 100 µL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Flow Cytometry (1-2ug/million cells)
Western Blot (1-2ug/ml)
Immunohistochemistry (Formalin-fixed) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes)
Optimal dilution for a specific application should be determined.

Validation Images & Assay Conditions

Gene/Protein Information For ESR1 (Source: Uniprot.org, NCBI)

Gene Name

ESR1

Full Name

Estrogen receptor

Weight

66.216kDa

Superfamily

nuclear hormone receptor family

Alternative Names

ER alpha; ER; Era; ER-alpha; ESR1; ESRESRA; Estradiol receptor; estrogen receptor 1; estrogen receptor alpha delta 3*4,56,7*/819-2 isoform; estrogen receptor alpha delta 4 +49 isoform; estrogen receptor alpha delta 4*5,6,7*/654 isoform; estrogen receptor alpha; estrogen receptor; NR3A1; NR3A1DKFZp686N23123; Nuclear receptor subfamily 3 group A member 1 ESR1 ER, ESR, ESRA, ESTRR, Era, NR3A1 estrogen receptor 1 estrogen receptor|E2 receptor alpha|ER-alpha|estradiol receptor|estrogen nuclear receptor alpha|estrogen receptor alpha E1-E2-1-2|estrogen receptor alpha E1-N2-E2-1-2|nuclear receptor subfamily 3 group A member 1|oestrogen receptor alpha

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ESR1, check out the ESR1 Infographic

ESR1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ESR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) ESR1 Monoclonal Antibody

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for mammary gland using anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-16

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-16

Question

Is a blocking peptide available for product anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody (M00057-1)?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

We do provide the blocking peptide for product anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody (M00057-1). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-09

Question

I was wanting to use your anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody for Flow Cytometry for human mammary gland on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human mammary gland identification?

Verified Customer

Verified customer

Asked: 2019-10-14

Answer

As indicated on the product datasheet, M00057-1 anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human mammary gland in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-10-14

Question

Does M00057-1 anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

R. Brown

Verified customer

Asked: 2018-09-26

Answer

As indicated on the product datasheet, M00057-1 anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2018-09-26

Question

Does anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1 work on canine Flow Cytometry with endometrium?

Verified Customer

Verified customer

Asked: 2018-06-29

Answer

Our lab technicians have not validated anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine endometrium in Flow Cytometry, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-06-29

Question

Is there a BSA free version of anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1 available?

Verified Customer

Verified customer

Asked: 2018-03-07

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-03-07

Question

I see that the anti-Estrogen Receptor, alpha (Marker of Estrogen Dependence) Monoclonal antibody M00057-1 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Z. Jones

Verified customer

Asked: 2015-10-26

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-10-26

Order DetailsPrice
M00057-1-100ug

100 μg

$468

Free Secondary Antibody

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M00057-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$468.00

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