|Product Name||Anti-Estrogen Receptor β Antibody|
|Storage & Handling||-20°C|
|Description||Rabbit Polyclonal antibody for ESR2 detection. Tested positive for IF, IHC, IHC, WB in Human, Mouse, Rat|
|Cite This Product||Anti-Estrogen Receptor β Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00786)|
|Contents/Buffer||Liquid. In PBS containing 1mg/ml BSA and 0.05% sodium azide.|
|Immunogen||Synthetic peptide corresponding to aa 100-115 (A100EPQKSPWCEARSLEH115) of human, mouse and rat ERβ (estrogen receptor β) .|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Gel Shift Assay (1-5µg/ml)
Immunohistochemistry (paraffin sections, 1-2µg/ml)
Western Blot (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Images And Assay Conditions
Figure 2. IHC analysis of ESR2 using anti-ESR2 antibody (A00786).
ESR2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ESR2 Antibody (A00786) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1: Estrogen receptor β, pAb (Prod. No. A00786) used in immunofluorescence.
Protein Target Info (Source: Uniprot.org)
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Research Areas||Cancer, Chip'Ing Antibodies, Diabetes, Domain Families, Endocrine System, Epigenetics And Nuclear Signaling, Metabolism, Neuroscience, Nuclear Hormone Receptors, Nuclear Receptors, Nuclear Signaling Pathways, Obesity, Signal Transduction, Signaling Pathway, Transcription, Tumor Biomarkers, Zinc Finger
*You can search these to find other products in these research areas.
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