SKU A00786
Size 50µg
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Applications IF, IHC, WB

Overview

Product Name Anti-Estrogen Receptor β Antibody
SKU/Catalog Number A00786
Storage & Handling -20°C
Size 50µg
Description Rabbit Polyclonal antibody for ESR2 detection. Tested positive for IF, IHC, IHC, WB in Human, Mouse, Rat
Cite This Product Anti-Estrogen Receptor β Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00786)
Host Rabbit
Immunogen Synthetic peptide corresponding to aa 100-115 (A100EPQKSPWCEARSLEH115) of human, mouse and rat ERβ  (estrogen receptor β) .
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Gel Shift Assay (1-5µg/ml)
Immunocytochemistry (2-4µg/ml)
Immunohistochemistry (paraffin sections, 1-2µg/ml)
Western Blot (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.

Images And Assay Conditions

/A/0/A00786 ESR2 IHC test 2.gif

Figure 2. IHC analysis of ESR2 using anti-ESR2 antibody (A00786).
ESR2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ESR2 Antibody (A00786) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

/A/0/A00786 ESR2 IF test 1.gif

Figure 1: Estrogen receptor β, pAb (Prod. No. A00786) used in immunofluorescence.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id Q92731
Gene Name ESR2

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Order Product (A00786)

Promotion:

Buy primary get secondary antibody for free.
Option Price
50µg $563
50µg+Free HRP Secondary BA1054 $563
50µg+Free Biotin Secondary BA1003 $563

USD $563

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Troubleshooting

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