|Product Name||Anti-Estrogen Receptor β Antibody|
|Storage & Handling||-20°C|
|Description||Rabbit Polyclonal antibody for ESR2 detection. Tested positive for IF, IHC, IHC, WB in Human, Mouse, Rat|
|Cite This Product||Anti-Estrogen Receptor β Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00786)|
|Immunogen||Synthetic peptide corresponding to aa 100-115 (A100EPQKSPWCEARSLEH115) of human, mouse and rat ERβ (estrogen receptor β) .|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Immunohistochemistry (paraffin sections, 1-2µg/ml)
Western Blot (1-2µg/ml)
Suggested dilutions/conditions may not be available for all applications.
Optimal conditions must be determined individually for each application.
Images And Assay Conditions
Figure 2. IHC analysis of ESR2 using anti-ESR2 antibody (A00786).
ESR2 was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-ESR2 Antibody (A00786) overnight at 4°C. Biotinylated goat anti Rabbit IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Figure 1: Estrogen receptor β, pAb (Prod. No. A00786) used in immunofluorescence.
Protein Target Info (Source: Uniprot.org)
*if product is indicated to react with multiple species, protein info is based on the human gene.
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