Anti-Exonuclease 1/EXO1 Picoband™ Antibody
|Applications||ELISA, Flow Cytometry, WB|
|Product Name||Anti-Exonuclease 1/EXO1 Picoband™ Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Exonuclease 1/EXO1 detection. Tested with WB, FCM, Direct ELISA in Human.|
|Cite This Product||Anti-Exonuclease 1/EXO1 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00536-2)|
|Contents/Buffer||Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Immunogen||E.coli-derived human Exonuclease 1/EXO1 recombinant protein (Position: M1-K294).|
Assay Dilutions Overview
Western blot, 0.25-0.5μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human
Direct ELISA, 0.1-0.5μg/ml, Human
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Images And Assay Conditions
Figure 2. Flow Cytometry analysis of 293T cells using anti-EXO1 antibody (A00536-2).
Overlay histogram showing 293T cells stained with A00536-2 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EXO1 Antibody (A00536-2,1Î¼g/1x106 cells) for 30 min at 20Â°C. DyLightÂ®488 conjugated goat anti-rabbit IgG (BA1127, 5-10Î¼g/1x106 cells) was used as secondary antibody for 30 minutes at 20Â°C. Isotype control antibody (Green line) was rabbit IgG (1Î¼g/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Figure 1. Western blot analysis of EXO1 using anti-EXO1 antibody (A00536-2).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human placenta tissue lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human MDA-MB-453 whole cell lysates,
Lane 4: human PC-3 whole cell lysates,
Lane 5: human PANC-1 whole cell lysates,
Lane 6: human HEK293 whole cell lysates,
Lane 7: human U2OS whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EXO1 antigen affinity purified polyclonal antibody (Catalog # A00536-2) at 0.5 Î¼g/mL overnight at 4Â°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for EXO1 at approximately 115KD. The expected band size for EXO1 is at 94KD.
Protein Target Info (Source: Uniprot.org)
|Protein Name||exonuclease 1|
|Tissue Specificity||Highly expressed in bone marrow, testis and thymus. Expressed at lower levels in colon, lymph nodes, ovary, placenta, prostate, small intestine, spleen and stomach.|
|Alternative Names||Exodeoxyribonuclease 1; Exodeoxyribonuclease I; EXO I; Exonuclease I; Protein DHS1; EXO1; DHS1; YOR033C; OR26.23|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||5'->3' double-stranded DNA exonuclease which may also possess a cryptic 3'->5' double-stranded DNA exonuclease activity. Functions in DNA mismatch repair (MMR) to excise mismatch-containing DNA tracts directed by strand breaks located either 5' or 3' to the mismatch. Also exhibits endonuclease activity against 5'-overhanging flap structures similar to those generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Required for somatic hypermutation (SHM) and class switch recombination (CSR) of immunoglobulin genes. Essential for male and female meiosis.|
*You can search these to find other products in these research areas.
|Background||Exonuclease 1 is an enzyme that in humans is encoded by the EXO1 gene. It is mapped to 1q43. This gene encodes a protein with 5' to 3' exonuclease activity as well as an RNase H activity. It is similar to the Saccharomyces cerevisiae protein Exo1 which interacts with Msh2 and which is involved in mismatch repair and recombination. Alternative splicing of this gene results in three transcript variants encoding two different isoforms.|
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