Anti-Factor I/CFI Antibody Picoband™

Complement Factor I antibody

Boster Bio Anti-Factor I/CFI Antibody Picoband™ catalog # PB9935. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Rat.

Product Info Summary

SKU: PB9935
Size: 100 μg/vial
Reactive Species: Human, Rat
Host: Rabbit
Application: Flow Cytometry, IHC, ICC, WB

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Product Name

Anti-Factor I/CFI Antibody Picoband™

View all Complement Factor I Antibodies

SKU/Catalog Number

PB9935

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Factor I/CFI Antibody Picoband™ catalog # PB9935. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Factor I/CFI Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9935)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human Factor I recombinant protein (Position: K19-D220). Human Factor I shares 70.7% and 71.2% amino acid (aa) sequence identity with mouse and rat Factor I, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9935 is reactive to CFI in Human, Rat

Applications

PB9935 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

75 kDa, 45 kDa

Calculated molecular weight

65.75kDa

Background of Complement Factor I

Complement factor I, also known as C3b/C4b inactivator, is a protein that in humans is encoded by the CFI gene. This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Rat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry, 0.5-1μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For CFI (Source: Uniprot.org, NCBI)

Gene Name

CFI

Full Name

Complement factor I

Weight

65.75kDa

Superfamily

peptidase S1 family

Alternative Names

AHUS3; C3b Inactivator; C3B/C4B inactivator; C3BINA; C3b-inactivator; CFI; complement component I; complement control protein factor I; complement factor I heavy chain; Complement Factor I; EC 3.4.21; EC 3.4.21.45; FIC3b-INA; I factor (complement); IF; KAF; Konglutinogen-activating factor; light chain of factor I CFI AHUS3, ARMD13, C3BINA, C3b-INA, FI, IF, KAF complement factor I complement factor I|C3B/C4B inactivator|C3b-inactivator|Konglutinogen-activating factor|complement component I|complement control protein factor I|complement factor I heavy chain|light chain of factor I

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CFI, check out the CFI Infographic

CFI infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CFI: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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3 Customer Q&As for Anti-Factor I/CFI Antibody Picoband™

Question

We are currently using anti-Factor I/CFI antibody PB9935 for rat tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-15

Answer

The anti-Factor I/CFI antibody (PB9935) has not been tested for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-15

Question

I was wanting to use your anti-Factor I/CFI antibody for ICC for rat visceral pleura on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat visceral pleura identification?

Verified Customer

Verified customer

Asked: 2018-03-22

Answer

It shows on the product datasheet, PB9935 anti-Factor I/CFI antibody has been tested for Flow Cytometry, IHC, ICC, WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat visceral pleura in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-03-22

Question

I am looking for to test anti-Factor I/CFI antibody PB9935 on rat visceral pleura for research purposes, then I may be interested in using anti-Factor I/CFI antibody PB9935 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

B. Wu

Verified customer

Asked: 2018-03-14

Answer

The products we sell, including anti-Factor I/CFI antibody PB9935, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2018-03-14

Order DetailsPrice
PB9935

100μg

$370
PB9935-10ug

10μg sample (liquid)

$99
PB9935-Biotin

100 μg Biotin conjugated

$570
PB9935-Cy3

100 μg Cy3 conjugated

$570
PB9935-Dylight488

100 μg Dylight488 conjugated

$570
PB9935-Dylight550

100 μg Dylight550 conjugated

$570
PB9935-Dylight594

100 μg Dylight594 conjugated

$570
PB9935-FITC

100 μg FITC conjugated

$570
PB9935-HRP

100 μg HRP conjugated

$570
PB9935-APC

100 μg APC conjugated

$670
PB9935-PE

100 μg PE conjugated

$670
PB9935-carrier-free

Carrier Free

$370

More conjugates/formats

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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