Overview
| Product Name |
Anti-Factor IX F9 Antibody
See more Factor IX products |
| Catalog# |
A00537 |
| Pack Size |
100μl |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Boster Bio Anti-Factor IX F9 Antibody catalog # A00537. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μl in Liquid form antibody. |
| Cite This Product |
Anti-Factor IX F9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00537)
|
| Antibodies Validation |
Antibodies Validation Information
|
Product Specs
| Host |
Rabbit |
| Reactive Species |
Human, Mouse, Rat |
| Applications |
WB
*Our Boster Guarantee covers the use of this product in the above
tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products. |
| Immunogen |
Synthesized peptide derived from Factor IX at AA range: 412-461. |
Gene/Protein Basic Information For F9 (Source: Uniprot.org, NCBI)
| Uniprot Id | P00740 |
|---|
| NCBI Gene Id | 2158 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | F9 |
|---|
| Protein Name | Coagulation factor IX |
|---|
| Superfamily | peptidase S1 family |
|---|
| Alternative Names | Factor IX|Christmas Factor; Coagulation Factor IX; EC 3.4.21; EC 3.4.21.22; F9; factor 9; FIX; HEMB; MGC129641; MGC129642; P19; Plasma Thromboplastic Component; Plasma thromboplastin component; PTC; THPH8 |
|---|
| Molecular Weight | 51778 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on F9, check out the F9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for F9: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the F9 infographic
Our Boster Quality Guarantee for Anti-Factor IX F9 Antibody covers its use in the following applications.
WB, 1:500-2000
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Western Blot (WB) analysis of 3T3 HepG2 cells using Factor IX Polyclonal antibody diluted at 1:800.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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That Boster Bio offers comprehensive selection of Western blot and IHC reagents? Great quality and save up to 50% cost.
Other Recommended Resources
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Questions and answers from customers related to A00537 Anti-Factor IX F9 Antibody
5 Related Questions
Question
Does anti-Factor IX antibody A00537 work on zebrafish WB with liver?
Verified Customer
Asked: 2019-10-16
Answer
Our lab technicians have not tested anti-Factor IX antibody A00537 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-Factor IX antibody A00537 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish liver in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-16
Question
Is a blocking peptide available for product anti-Factor IX antibody (A00537)?
Verified Customer
Asked: 2019-10-04
Answer
We do provide the blocking peptide for product anti-Factor IX antibody (A00537). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-04
Question
Does A00537 anti-Factor IX antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Asked: 2018-10-18
Answer
As indicated on the product datasheet, A00537 anti-Factor IX antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-10-18
Question
I have attached the WB image, lot number and protocol we used for liver using anti-Factor IX antibody A00537. Please let me know if you require anything else.
D. Krishna
Asked: 2015-05-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-05-06
Question
We are currently using anti-Factor IX antibody A00537 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
N. Krishna
Asked: 2013-02-22
Answer
The anti-Factor IX antibody (A00537) has not been validated for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2013-02-22
