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SKU:PA2061
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:WB
Price: $200.00
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Overview

Product Name Anti-Factor VIII Antibody
SKU/Catalog Number PA2061
Description Rabbit IgG polyclonal antibody for Coagulation factor VIII(F8) detection. Tested with WB in Human.
Cite This Product Anti-Factor VIII Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2061)
Replacement Item This antibody may replace the following items: sc-19665|sc-27647|sc-27649|sc-27650|sc-27651|sc-33583|sc-33584|sc-52026|sc-53465|sc-53466|sc-59508|sc-59510|sc-59512|sc-59514|sc-65947|sc-65948|sc-65949|sc-65950|sc-73597 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human
Application WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence in the middle region of human Factor VIII(1264-1278aa APVLQDFRSLNDSTN).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name F8
Protein Name Coagulation factor VIII
Molecular Weight 267009 MW
Protein Function Factor VIII, along with calcium and phospholipid, acts as a cofactor for factor IXa when it converts factor X to the activated form, factor Xa.
Sequence Similarities Belongs to the multicopper oxidase family.
Subcellular Localization Secreted, extracellular space.
Uniprot ID P00451
Alternative Names Coagulation factor VIII;Antihemophilic factor;AHF;Procoagulant component;Factor VIIIa heavy chain, 200 kDa isoform;Factor VIIIa heavy chain, 92 kDa isoform;Factor VIII B chain;Factor VIIIa light chain;F8;F8C;
Research Areas |cardiovascular|blood|serum proteins| cardiovascular|platelets|coagulation|extrinsic|regulatory| cancer|invasion/microenvironment|angiogenesis|angiogenic growth factors| stem cells|endothelial progenitors|endothelial markers|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Coagulation factor VIII

Coagulation Factor VIII(FVIII) is an essential blood-clotting protein, also known as anti-hemophilic factor(AHF). By in situ hybridization, Tantravahi et al.(1986) concluded that the F8 gene is located in the proximal part of chromosome Xq28 with probes DX13 and St14 distally located. The F8 gene encodes coagulation factor VIII, a large plasma glycoprotein that functions in the blood coagulation cascade as a cofactor for the factor IXa-dependent activation of factor X(F10). Factor VIII is activated proteolytically by a variety of coagulation enzymes, including thrombin(F2). Factor VIII is tightly associated in the blood with von Willebrand factor(VWF), which serves as a protective carrier protein for factor VIII(Toole et al., 1984; Hoyer, 1994).

Anti-Factor VIII Antibody Images

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Anti-Factor VIII Antibody
Anti-Factor VIII antibody, PA2061, Western blotting
Lane 1: A431 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SMMC Cell Lysate
Lane 4: JURKAT Cell Lysate
Lane 5: RAJI Cell Lysate
Lane 6: CEM Cell Lysate
Lane 7: HL-60 Cell Lysate
Lane 8: MCF-7 Cell Lysate
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Publications

Zhang X, Shen Mr, Xu Zd, Hu Z, Chen C, Chi Yl, Kong Zd, Li Zf, Li Xt, Guo Sl, Xiong Sh, Zhang Cs. Exp Biol Med (Maywood). 2014 May;239(5):628-37. Doi: 10.1177/1535370214525323. Epub 2014 Mar 27. Cardiomyocyte Differentiation Induced In Cardiac Pro...
Li S, Qu H, Hao J, Sun J, Guo H, Guo C, Sun B, Tu C. Biochim Biophys Acta. 2010 Sep;1804(9):1882-8. Doi: 10.1016/J.Bbapap.2010.05.011. Epub 2010 Jun 1. Proteomic Analysis Of Primary Porcine Endothelial Cells After Infection By Classical Swine Feve...
Li Y, Cao Y, Zeng Z, Liang M, Xue Y, Xi C, Zhou M, Jiang W. Sci Rep. 2015 Feb 3;5:8209. Doi: 10.1038/Srep08209. Angiotensin-Converting Enzyme 2/Angiotensin-(1-7)/Mas Axis Prevents Lipopolysaccharide-Induced Apoptosis Of Pulmonary Microvascular End...
Zhang E, Shen F,. J Biomed Mater Res A. 2015 Aug;103(8):2758-69. Doi: 10.1002/Jbm.A.35410. Epub 2015 Feb 24. A Ferulic Acid (Fa)-Eluting System For Biodegradable Magnesium Stent: Cells Response Of Huvecs.
You Qh, Sun Gy, Wang N, Shen Jl, Wang Y. J Surg Res. 2010 Jul;162(1):110-21. Doi: 10.1016/J.Jss.2009.01.019. Epub 2009 Feb 8. Interleukin-17F-Induced Pulmonary Microvascular Endothelial Monolayer Hyperpermeability Via The Protein Kinase C Pathway.
Feng D, Peng C, Li C, Zhou Y, Li M, Ling B, Wei H, Tian Z. Oncol Rep. 2009 Nov;22(5):1129-34. Identification And Characterization Of Cancer Stem-Like Cells From Primary Carcinoma Of The Cervix Uteri.
Wang J, Chen Y, Lin C, Jia J, Tian L, Yang K, Zhao L, Lai N, Jiang Q, Sun Y, Zhong N, Ran P, Lu W. Am J Physiol Cell Physiol. 2014 Feb 15;306(4):C364-73. Doi: 10.1152/Ajpcell.00048.2013. Epub 2013 Dec 11. Effects Of Chronic Exposure To Cigarette S...
Liu X, Zhang G, Hou C, Wang H, Yang Y, Guan G, Dong W, Gao H, Feng Q. Biomed Res Int. 2013;2013:854917. Doi: 10.1155/2013/854917. Epub 2013 Dec 4. Vascularized Bone Tissue Formation Induced By Fiber-Reinforced Scaffolds Cultured With Osteoblasts A...
Huang J, Li Y, Qi Y, Zhang Y, Zhang L, Wang Z, Zhang X, Gui L. Mol Cell Biochem. 2014 Dec;397(1-2):157-65. Doi: 10.1007/S11010-014-2183-3. Epub 2014 Aug 20. Coordinated Regulation Of Autophagy And Apoptosis Determines Endothelial Cell Fate During ...
You Qh, Sun Gy, Wang N, Chen S, Luo Ql. Inflamm Res. 2010 Nov;59(11):949-58. Doi: 10.1007/S00011-010-0207-3. Epub 2010 May 8. Role Of Src-Suppressed C Kinase Substrate In Rat Pulmonary Microvascular Endothelial Hyperpermeability Stimulated By Infl...
Hao Jh, Yu M, Li Hk, Shi Yr, Li Q, Hao Xs. World J Gastroenterol. 2006 Feb 21;12(7):1140-3. Inhibitory Effect Of Antisense Vascular Endothelial Growth Factor Rna On The Profile Of Hepatocellular Carcinoma Cell Line In Vitro And In Vivo.
Wu Y, Chen W, Guo M, He Q, Hu Y. Int J Clin Exp Pathol. 2014 Jul 15;7(8):4827-36. Ecollection 2014. Effects Of Transforming Growth Factor-??2 On Myocilin Expression And Secretion In Human Primary Cultured Trabecular Meshwork Cells.
Jiang H, Deng Xf, Duan Cm, Chen C, Xiang Jl, Lu Yl, Ma Qf. Lymphology. 2011 Mar;44(1):21-8. Somatostatin Receptors Sstr2 And Sstr5 Are Expressed In The Human Thoracic Duct.
Zhou D, Zhan S, Zhou D, Li Z, Lin X, Tang K, Shu H, Chen G, Zeng S, Cai Y, Jiang X. Cell Mol Neurobiol. 2011 Jul;31(5):687-94. Doi: 10.1007/S10571-011-9665-6. Epub 2011 Mar 9. A Study Of The Distribution And Density Of The Vegfr-2 Receptor On Glio...
Pan X, Chen Z, Huang R, Yao Y, Ma G. Plos One. 2013;8(4):E60335. Doi: 10.1371/Journal.Pone.0060335. Epub 2013 Apr 1. Transforming Growth Factor ??1 Induces The Expression Of Collagen Type I By Dna Methylation In Cardiac Fibroblasts.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to factor viii antibody, coagulation factor viii antibody, f8 antibody, fviii antibody