Product Info Summary
SKU: | A00422-1 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | ELISA, WB |
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Product info
Product Name
Anti-Fibroblast activation protein, alpha/FAP Antibody Picoband™
View all Fibroblast Activation Protein alpha/FAP Antibodies
SKU/Catalog Number
A00422-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Fibroblast activation protein, alpha/FAP Antibody Picoband™ catalog # A00422-1. Tested in ELISA, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fibroblast activation protein, alpha/FAP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00422-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human FAP recombinant protein (Position: R550-A619).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00422-1 is reactive to FAP in Human
Applications
A00422-1 is guaranteed for ELISA, WB Boster Guarantee
Observed Molecular Weight
95 kDa
Calculated molecular weight
87.713kDa
Background of Fibroblast Activation Protein alpha/FAP
Fibroblast activation protein alpha is a 170 kDa melanoma membrane-bound gelatinase, protein that in humans is encoded by the FAP gene. The protein encoded by this gene is a homodimeric integral membrane gelatinase belonging to the serine protease family. It is selectively expressed in reactive stromal fibroblasts of epithelial cancers, granulation tissue of healing wounds, and malignant cells of bone and soft tissue sarcomas. This protein is thought to be involved in the control of fibroblast growth or epithelial-mesenchymal interactions during development, tissue repair, and epithelial carcinogenesis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Direct ELISA, 0.1-0.5μg/ml
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of FAP using anti-FAP antibody (A00422-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human A375 cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAP antigen affinity purified polyclonal antibody (Catalog # A00422-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for FAP at approximately 95KD. The expected band size for FAP is at 88KD.
Protein Target Info & Infographic
Gene/Protein Information For FAP (Source: Uniprot.org, NCBI)
Gene Name
FAP
Full Name
Prolyl endopeptidase FAP
Weight
87.713kDa
Superfamily
peptidase S9B family
Alternative Names
170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast Activation Protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase; vibronectin FAP DPPIVA, FAPalpha, SIMP, FAP fibroblast activation protein alpha prolyl endopeptidase FAP|170 kDa melanoma membrane-bound gelatinase|dipeptidyl peptidase FAP|gelatine degradation protease FAP|integral membrane serine protease|post-proline cleaving enzyme|seprase|surface-expressed protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FAP, check out the FAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fibroblast activation protein, alpha/FAP Antibody Picoband™ (A00422-1)
Hello CJ!
A00422-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Jin X,Wei M,Wang S,Wang G,Lai Y,Shi Y,Zhang Y,Yang Z,Wang X.Detecting fibroblast activation proteins in lymphoma using 68Ga-FAPI PET/CT.J Nucl Med.2021 May 28:jnumed.121.262134.doi:10.2967/jnumed.121.262134.Epub ahead of print.PMID:34049984.
Species: Human
A00422-1 usage in article: APP:IHC, SAMPLE:LYMPHOMA TISSUE, DILUTION:NA
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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15 Customer Q&As for Anti-Fibroblast activation protein, alpha/FAP Antibody Picoband™
Question
We are currently using anti-Fibroblast activation protein, alpha/FAP antibody A00422-1 for mouse tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
The anti-Fibroblast activation protein, alpha/FAP antibody (A00422-1) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-01-10
Question
Do you have a BSA free version of anti-Fibroblast activation protein, alpha/FAP antibody A00422-1 available?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Fibroblast activation protein, alpha/FAP antibody A00422-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-13
Question
Can you help my question with product A00422-1, anti-Fibroblast activation protein, alpha/FAP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00422-1 anti-Fibroblast activation protein, alpha/FAP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-10-23
Question
Is a blocking peptide available for product anti-Fibroblast activation protein, alpha/FAP antibody (A00422-1)?
Verified Customer
Verified customer
Asked: 2019-10-11
Answer
We do provide the blocking peptide for product anti-Fibroblast activation protein, alpha/FAP antibody (A00422-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-11
Question
Is this A00422-1 anti-Fibroblast activation protein, alpha/FAP antibody reactive to the isotypes of FAP?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The immunogen of A00422-1 anti-Fibroblast activation protein, alpha/FAP antibody is E. coli-derived human FAP recombinant protein (Position: R550-A619). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-10
Question
Would anti-Fibroblast activation protein, alpha/FAP antibody A00422-1 work for ELISA with fibroblast?
Verified Customer
Verified customer
Asked: 2019-06-27
Answer
According to the expression profile of fibroblast, FAP is highly expressed in fibroblast. So, it is likely that anti-Fibroblast activation protein, alpha/FAP antibody A00422-1 will work for ELISA with fibroblast.
Boster Scientific Support
Answered: 2019-06-27
Question
My lab would like to test anti-Fibroblast activation protein, alpha/FAP antibody A00422-1 on rat fibroblast for research purposes, then I may be interested in using anti-Fibroblast activation protein, alpha/FAP antibody A00422-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
The products we sell, including anti-Fibroblast activation protein, alpha/FAP antibody A00422-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-22
Question
Here is the WB image, lot number and protocol we used for fibroblast using anti-Fibroblast activation protein, alpha/FAP antibody A00422-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-16
Question
We purchased anti-Fibroblast activation protein, alpha/FAP antibody for WB on body of uterus a few months ago. I am using rat, and We intend to use the antibody for ELISA next. We want examining body of uterus as well as melanoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2019-04-29
Answer
I looked at the website and datasheets of our anti-Fibroblast activation protein, alpha/FAP antibody and I see that A00422-1 has been validated on rat in both WB and ELISA. Thus A00422-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-04-29
Question
I see that the anti-Fibroblast activation protein, alpha/FAP antibody A00422-1 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-03-12
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-03-12
Question
We were satisfied with the WB result of your anti-Fibroblast activation protein, alpha/FAP antibody. However we have seen positive staining in plasma prolyl endopeptidase fap: cell surface using this antibody. Is that expected? Could you tell me where is FAP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-10-25
Answer
From what I have seen in literature, plasma does express FAP. Generally FAP expresses in prolyl endopeptidase fap: cell surface, antiplasmin-cleaving enzyme fap, soluble, isoform 2: cytoplasm. Regarding which tissues have FAP expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 7911242
Melanoma, Pubmed ID: 9065413, 9247085, 10644713
Placenta, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2018-10-25
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fibroblast using anti-Fibroblast activation protein, alpha/FAP antibody A00422-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-01
Question
Would A00422-1 anti-Fibroblast activation protein, alpha/FAP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Williams
Verified customer
Asked: 2017-07-11
Answer
It shows on the product datasheet, A00422-1 anti-Fibroblast activation protein, alpha/FAP antibody as been validated on ELISA. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-07-11
Question
I was wanting to use your anti-Fibroblast activation protein, alpha/FAP antibody for ELISA for rat fibroblast on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat fibroblast identification?
R. Lewis
Verified customer
Asked: 2014-08-19
Answer
As indicated on the product datasheet, A00422-1 anti-Fibroblast activation protein, alpha/FAP antibody has been tested for ELISA, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fibroblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-08-19
Question
We have observed staining in mouse body of uterus. What should we do? Is anti-Fibroblast activation protein, alpha/FAP antibody supposed to stain body of uterus positively?
A. Jones
Verified customer
Asked: 2013-06-25
Answer
According to literature body of uterus does express FAP. According to Uniprot.org, FAP is expressed in body of uterus, fibroblast, melanoma, placenta, plasma, among other tissues. Regarding which tissues have FAP expression, here are a few articles citing expression in various tissues:
Fibroblast, Pubmed ID: 7911242
Melanoma, Pubmed ID: 9065413, 9247085, 10644713
Placenta, Pubmed ID: 15489334
Plasma, Pubmed ID: 16335952
Boster Scientific Support
Answered: 2013-06-25