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SKU:PA1119
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-FAS/Cd95 Antibody
SKU/Catalog Number PA1119
Description Rabbit IgG polyclonal antibody for Tumor necrosis factor receptor superfamily member 6(FAS) detection. Tested with WB, IHC-P, IHC-F in Mouse;Rat.
Cite This Product Anti-FAS/Cd95 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1119)
Replacement Item This antibody may replace the following items: sc-74540|sc-7886|sc-21730|sc-715|sc-8009|sc-1023|sc-1024|sc-714|sc-716|sc-715-G|sc-714-G from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Mouse, Rat
Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of rat Fas (87-109aa, YTDRKHYSDKCRRCAFCDEGHGL).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Fas
Protein Name Tumor necrosis factor receptor superfamily member 6
Molecular Weight 36835 MW
Protein Function Receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death- inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. FAS- mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both (By similarity). .
Sequence Similarities Contains 1 death domain.
Subcellular Localization Membrane; Single-pass type I membrane protein.
Uniprot ID Q63199
Alternative Names Tumor necrosis factor receptor superfamily member 6;Apo-1 antigen;Apoptosis-mediating surface antigen FAS;FASLG receptor;CD95;Fas;Apt1, Tnfrsf6;
Research Areas |cell biology|apoptosis|extracellular signals|death ligands| immunology|innate immunity|cytokines|necrosis factors|tnf superfamily|immune system diseases|autoimmune| stem cells|hematopoietic progenitors|lymphoid|t lymphocytic lineage|adaptive immunity|regulatory t cells|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Tumor necrosis factor receptor superfamily member 6

FAS(also known as surface antigen APO1 or CD95) is a member of the tumour-necrosis receptor factor family of death receptors, can induce apoptosis or, conversely, can deliver growth stimulatory signals. It acts as an inducer of both neurite growth in vitro and accelerated recovery after nerve injury in vivo. Fas antigen is expressed and functional on papillary thyroid cancer cells and this may have potential therapeutic significance. The FAS antigen shows structural homology with a number of cell surface receptors, including tumor necrosis factor(TNF) receptors and the low-affinity nerve growth factor receptor(NGFR) and is mapped to 10q24.1. And the FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis.

Anti-FAS/Cd95 Antibody Images

Click the images to enlarge.

Anti-FAS/Cd95 Antibody
Anti-Fas antibody, PA1119, Western blotting
Lane 1: Recombinant Mouse FAS Protein 10ng
Lane 2: Recombinant Mouse FAS Protein 5ng
Lane 3: Recombinant Mouse FAS Protein 2.5ng
Anti-FAS/Cd95 Antibody
Anti-Fas antibody, PA1119, IHC(P)
IHC(P): Rat Spleen Tissue Lysate
Anti-FAS/Cd95 Antibody
Western blot analysis of FAS expression in rat liver extract (lane 1), rat spleen extract (lane 2), rat brain extract (lane 3) and rat cardiac muscle extract (lane 4). FAS at 50KD was detected using rabbit anti- FAS Antigen Affinity purified polyclonal antibody (Catalog # PA1119) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Anti-FAS/Cd95 Antibody
Western blot analysis of FAS expression in mouse liver extract (lane 1), mouse spleen extract (lane 2), mouse brain extract (lane 3) mouse kidney extract (lane 4), mouse thymus extract (lane 5), mouse lung extract (lane 6), HEPA1-6 whole cell lysates (lane 7) and NIH3T3 whole cell lysates (lane 8). FAS at 50KD was detected using rabbit anti- FAS Antigen Affinity purified polyclonal antibody (Catalog # PA1119) at 0.5 μg/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
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Publications

Yeung CK, Wang G, Yao Y, Liang J, Tenny Chung CY, Chuai M, Lee KK, Yang X. Cell Death Dis. 2017 Mar 23;8(3):e2697. doi: 10.1038/cddis.2017.91. BRE modulates granulosa cell death to affect ovarian follicle development and atresia in the mouse.
Weng T, Xie Y, Yi L, Huang J, Luo F, Du X, Chen L, Liu C, Chen D, Chen L. Osteoarthritis Cartilage. 2014 Aug;22(8):1197-205. Doi: 10.1016/J.Joca.2014.06.031. Epub 2014 Jul 4. Loss Of Vhl In Cartilage Accelerated The Progression Of Age-Associated A...
Li W, Xia D, Wang Y, Li Y, Xue Y, Wu X, Ye Z. J Huazhong Univ Sci Technolog Med Sci. 2011 Dec;31(6):794-8. Doi: 10.1007/S11596-011-0679-6. Epub 2011 Dec 16. Relationship Between Aberrant Methylation Of Fas Promoter And Biological Behavior Of Bladd...
Liu Wl, Liu Zw, Li Ts, Wang C, Zhao B. Chin Med J (Engl). 2013 Feb;126(3):494-9. Hydrogen Sulfide Donor Regulates Alveolar Epithelial Cell Apoptosis In Rats With Acute Lung Injury.
Ma H, Zhang X, Zhang X, Yang D, Meng L, Zhang Y, Zhou S. Arch Med Sci. 2013 Apr 20;9(2):354-60. Doi: 10.5114/Aoms.2012.31439. Epub 2012 Oct 30. The Effect Of Esculentoside A On Lupus Nephritis-Prone Bxsb Mice.
Liu Mw, Su Mx, Zhang W, Zhang Lm, Wang Yh, Qian Cy. Int J Mol Med. 2015 Aug;36(2):386-98. Doi: 10.3892/Ijmm.2015.2241. Epub 2015 Jun 10. Rhodiola Rosea Suppresses Thymus T-Lymphocyte Apoptosis By Downregulating Tumor Necrosis Factor-??-Induced Prot...
Kan S, Zhou H, Jin C, Yang H. Int J Clin Exp Med. 2015 Mar 15;8(3):3258-70. Ecollection 2015. Effects Of Pdtc On Nf-??b Expression And Apoptosis In Rats With Severe Acute Pancreatitis-Associated Lung Injury.
Li B, Chu X, Gao M, Li W. Acta Biochim Biophys Sin (Shanghai). 2010 Jan;42(1):80-9. Apoptotic Mechanism Of Mcf-7 Breast Cells In Vivo And In Vitro Induced By Photodynamic Therapy With C-Phycocyanin.
Li Q, Peng J, Li Xh, Liu T, Liang Qc, Zhang Gy. World J Gastroenterol. 2010 Mar 14;16(10):1274-8. Clinical Significance Of Fas And Fasl Protein Expression In Gastric Carcinoma And Local Lymph Node Tissues.
Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.
Li B, Xu Yj, Chu Xm, Gao Mh, Wang Xh, Nie Sm, Yang F, Lv Cy. Immunol Lett. 2013 Nov-Dec;156(1-2):68-81. Doi: 10.1016/J.Imlet.2013.09.025. Epub 2013 Sep 29. Molecular Mechanism Of Inhibitory Effects Of Cd59 Gene On Atherosclerosis In Apoe (-/-) Mice.
Qin Jh, Ma Jz, Yang Xw, Hu Yj, Zhou J, Fu Lc, Tian Rh, Liu S, Xu G, Shen Xl. Nat Prod Bioprospect. 2015 Jun;5(3):159-66. Doi: 10.1007/S13659-015-0063-5. Epub 2015 Jun 16. A Triterpenoid Inhibited Hormone-Induced Adipocyte Differentiation And Allev...
Wu Yh, Hu Sq, Liu J, Cao Hc, Xu W, Li Yj, Li Lj. Int J Mol Med. 2014 Jun;33(6):1498-506. Doi: 10.3892/Ijmm.2014.1730. Epub 2014 Apr 7. Nature And Mechanisms Of Hepatocyte Apoptosis Induced By D-Galactosamine/Lipopolysaccharide Challenge In Mice.
Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.