Anti-Fas Ligand/FASLG Antibody
Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P in Human. Cited in 11 publication(s).
Anti-Fas Ligand/FASLG Antibody Info At A Glance
|Product Name||Anti-Fas Ligand/FASLG Antibody
See all Fas Ligand/TNFSF6 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Fas Ligand/FASLG detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. Fas Ligand/FASLG information: Molecular Weight: 31485 MW; Subcellular Localization: Cell membrane; Single-pass type II membrane protein. Cytoplasmic vesicle lumen. Lysosome lumen. Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. Colocalizes with the SPPL2A protease at the cell membrane.|
|Cite This Product||Anti-Fas Ligand/FASLG Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1576)|
|Similar Products From
|Anti-Fas Ligand/FASLG Antibody may replace the following items: sc 18897|sc 19681|sc 33716|sc 6237|sc 71096|sc 834|sc 834 G|sc 956|sc 956 G|sc 957.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
*Innovating Scientists reward: if you test this antibody on a species or application not listed above and share with us your results, we will provide you a full credit to purchase Boster products.
|Related Products||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information.
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion all year round.
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Fas Ligand(263-281aa ELSLVNFEESQTFFGLYKL).|
|Cross Reactivity||No cross reactivity with other proteins|
Gene/Protein Basic Information For FASLG (Source: Uniprot.org, NCBI)
|NCBI Gene Id||356|
|Species Of This Entry||Human|
|Protein Name||Tumor necrosis factor ligand superfamily member 6|
|Superfamily||tumor necrosis factor family|
|Alternative Names||Fas Ligand/TNFSF6|apoptosis (APO-1) antigen ligand 1; Apoptosis antigen ligand; APT1LG1CD95L; APTL; CD178 antigen; CD178; CD95L; CD95-L; Fas antigen ligand; Fas ligand (TNF superfamily, member 6); Fas Ligand; FASLCD95 ligand; FASLG; TNFSF6; TNFSF6FasL; tumor necrosis factor (ligand) superfamily, member 6; tumor necrosis factor ligand superfamily member 6|
|Post Tranlational Modifications||Cleavage; Phosphorylation; Dephosphorylation; Reduction; Acetylation; Methylation; Demethylation; Nitration; Oxidation; Deglycosylation; Prenylation; Ubiquitination; Deacetylation; Myristoylation|
|Subcellular Localization||Cell membrane; Single-pass type II membrane protein. Cytoplasmic vesicle lumen. Lysosome lumen. Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination (PubMed:17164290). Colocalizes with the SPPL2A protease at the cell membrane (PubMed:17557115).; [Tumor necrosis factor ligand superfamily member 6, soluble form]: Secreted. May be released into the extracellular fluid by cleavage from the cell surface.; [FasL intracellular domain]: Nucleus. The FasL ICD cytoplasmic form is translocated into the nucleus.|
|Gene Location||1q24.3, on Chromasome 1, gene sequence: NC_000001.11 (172659008..172666876)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For FASLG (Source: Uniprot.org, NCBI)
|Protein Function||Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells (PubMed:26334989, PubMed:9228058). Involved in cytotoxic T-cell-mediated apoptosis, natural killer cell-mediated apoptosis and in T-cell development (PubMed:9228058, PubMed:7528780, PubMed:9427603). Initiates fratricidal/suicidal activation-induced cell death (AICD) in antigen-activated T-cells contributing to the termination of immune responses (By similarity). TNFRSF6/FAS-mediated apoptosis has also a role in the induction of peripheral tolerance (By similarity). Binds to TNFRSF6B/DcR3, a decoy receptor that blocks apoptosis (PubMed:27806260).|
|Interacting Proteins||Ap2b1; CASP8; FADD; FAS; FNBP1; FYN; ITK; KRT40; NCK2; NOTCH2NL; PACSIN1; PACSIN2; PACSIN3; PIK3R1; PSTPIP1; RGS20; Snx18; SNX33; SNX9; SRGAP2; TRIP10; TRIP6|
|Related Diseases||Neoplasms; Malignant Neoplasms; Carcinoma; Autoimmune Reaction; Malignant Paraganglionic Neoplasm; Inflammation; Leukemia; Autoimmune Diseases; Adenocarcinoma; Lymphoma; Hiv Infections; Hepatitis; Neoplasm Metastasis; Immunologic Deficiency Syndromes; Glioma; Autoimmunity; Melanoma; Malignant Tumor Of Colon; Malignant Squamous Cell Neoplasm; Liver Neoplasms|
|Related Pathways||Cell Death; Pathogenesis; Induction Of Apoptosis; Immune Response; Programmed Cell Death; Cell Proliferation; Cell Cycle; Secretion; Cell Growth; Cell Activation; Localization; Sensitization; Inflammatory Response; Apoptotic Process; T Cell Activation; Cell Cycle Arrest; Reverse Transcription; Cytolysis; Cell Killing; Angiogenesis|
|Background||Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.|
|Scientific References||PMID: 7528780 by Alderson M.; Fas ligand mediates activation-induced cell death in human T lymphocytes.|
PMID: 7826947 by Takahashi T., et al. Human Fas ligand: gene structure, chromosomal location and species specificity.
Our Boster Quality Guarantee for Anti-Fas Ligand/FASLG Antibody covers its use in the following applications.Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
All lanes: Anti FASLG (PA1576) at 0.5ug/ml
Lane 1: JURKAT Whole Cell Lysate at 40ug
Lane 2: HL60 Whole Cell Lysate at 40ug
Predicted bind size: 31 KD
Observed bind size: 31 KD
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
- Western Blottting Resource Center
- Immunohistochemistry Resource Center
- Flow Cytometry Technical Resource Center
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 11
|Wu Yh, Hu Sq, Liu J, Cao Hc, Xu W, Li Yj, Li Lj. Int J Mol Med. 2014 Jun;33(6):1498-506. Doi: 10.3892/Ijmm.2014.1730. Epub 2014 Apr 7. Nature And Mechanisms Of Hepatocyte Apoptosis Induced By D-Galactosamine/Lipopolysaccharide Challenge In Mice.||PubMed ID 24714963|
|Jiang H, Li J, Zhou T, Wang C, Zhang H, Wang H. Int J Mol Med. 2014 May;33(5):1298-304. Doi: 10.3892/Ijmm.2014.1684. Epub 2014 Mar 6. Colistin-Induced Apoptosis In Pc12 Cells: Involvement Of The Mitochondrial Apoptotic And Death Receptor Pathways.||PubMed ID 24604244|
|Han X, Qiu L, Zhang Y, Kong Q, Wang H, Wang H, Li H, Duan C, Wang Y, Song Y, Wang C. Exp Biol Med (Maywood). 2009 May;234(5):595-603. Doi: 10.3181/0812-Rm-359. Epub 2009 Feb 25. Transplantation Of Sertoli-Islet Cell Aggregates Formed By Microgravi...||PubMed ID 19244542|
|Li Q, Peng J, Li Xh, Liu T, Liang Qc, Zhang Gy. World J Gastroenterol. 2010 Mar 14;16(10):1274-8. Clinical Significance Of Fas And Fasl Protein Expression In Gastric Carcinoma And Local Lymph Node Tissues.||PubMed ID 20222173|
|Liu Mw, Su Mx, Zhang W, Zhang Lm, Wang Yh, Qian Cy. Int J Mol Med. 2015 Aug;36(2):386-98. Doi: 10.3892/Ijmm.2015.2241. Epub 2015 Jun 10. Rhodiola Rosea Suppresses Thymus T-Lymphocyte Apoptosis By Downregulating Tumor Necrosis Factor-??-Induced Pro...||PubMed ID 26063084|
|Ma H, Zhang X, Zhang X, Yang D, Meng L, Zhang Y, Zhou S. Arch Med Sci. 2013 Apr 20;9(2):354-60. Doi: 10.5114/Aoms.2012.31439. Epub 2012 Oct 30. The Effect Of Esculentoside A On Lupus Nephritis-Prone Bxsb Mice.||PubMed ID 23671449|
|Wang J, Tang T, Yang H, Yao X, Chen L, Liu W, Li T. J Neurosurg Spine. 2007 May;6(5):425-30. The Expression Of Fas Ligand On Normal And Stabbed-Disc Cells In A Rabbit Model Of Intervertebral Disc Degeneration: A Possible Pathogenesis.||PubMed ID 17542508|
|Wang Xy, Zhang R, Lian S. Clin Exp Dermatol. 2011 Jan;36(1):69-76. Doi: 10.1111/J.1365-2230.2010.03860.X. Aberrant Expression Of Fas And Fasl Pro-Apoptotic Proteins In Basal Cell And Squamous Cell Carcinomas.||PubMed ID 20497185|
|Zhou X, Hong T, Yu Q, Nie S, Gong D, Xiong T, Xie M. Sci Rep. 2017 Oct 27;7(1):14247. doi: 10.1038/s41598-017-14178-2. Exopolysaccharides from Lactobacillus plantarum NCU116 induce c-Jun dependent Fas/Fasl-mediated apoptosis via TLR2 in mouse inte...||PubMed ID 29079852|
|Cisplatin-induced oxidative stress stimulates renal Fas ligand shedding||PubMed ID 29619879|
|Expression of Fas ligand and caspase-3 contributes to formation of immune escape in gastric cancer||PubMed ID 12854132|
Order Product (PA1576)
Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
All Boster antibodies and ELISA kits are guaranteed to meet the specifications on the data sheet.See Details
Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.Download Free PDFs Now
Guaranteed product quality
We promise all of our products perform as described in datasheets.