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SKU:PB9068
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $240.00
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Overview

Product Name Anti-FASL Antibody
SKU/Catalog Number PB9068
Description Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 6(FASLG) detection. Tested with WB, IHC-P in Mouse;Rat.
Cite This Product Anti-FASL Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9068)
Replacement Item This antibody may replace the following items: sc-18897|sc-19681|sc-33716|sc-6237|sc-71096|sc-834|sc-834-G|sc-956|sc-956-G|sc-957 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Mouse, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived mouse Fas Ligand recombinant protein (Position: Q128-L279). Mouse Fas Ligand shares 82% and 93% amino acid (aa) sequences identity with human and rat, respectively.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Faslg
Protein Name Tumor necrosis factor ligand superfamily member 6
Molecular Weight 31442 MW
Protein Function Cytokine that binds to TNFRSF6/FAS, a receptor that transduces the apoptotic signal into cells. May be involved in cytotoxic T-cell mediated apoptosis and in T-cell development. TNFRSF6/FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both. Binding to the decoy receptor TNFRSF6B/DcR3 modulates its effects (By similarity). .
Subcellular Localization Isoform FasL: Cell membrane; Single-pass type II membrane protein. Cytoplasmic vesicle lumen . Lysosome lumen . Is internalized into multivesicular bodies of secretory lysosomes after phosphorylation by FGR and monoubiquitination. Colocalizes with the SPPL2A protease at the cell membrane (By similarity). .
Uniprot ID P41047
Alternative Names Tumor necrosis factor ligand superfamily member 6;CD95 ligand;CD95-L;Fas antigen ligand;Fas ligand;FasL;CD178;Tumor necrosis factor ligand superfamily member 6, membrane form;Tumor necrosis factor ligand superfamily member 6, soluble form;Receptor-binding FasL ectodomain;Soluble Fas ligand;sFasL;ADAM10-processed FasL form;APL;FasL intracellular domain;FasL ICD;SPPL2A-processed FasL form;SPA;Faslg;Apt1lg1, Cd95l, Fasl, gld, Tnfsf6;
Research Areas |cell biology|apoptosis|receptors|death receptors| immunology|innate immunity|cytokines|tnf superfamily| cell biology|intracellular|caspases etc|caspases| stem cells|hematopoietic progenitors|lymphoid|t lymphocytic lineage| kits/ lysates/ other|elisa kits|cytokines and cytokine receptors elisa kits|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Tumor necrosis factor ligand superfamily member 6

Fas ligand (FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. It is mapped to 1q24.3. FASL may contribute to the immune privilege of tumors. Pharmacologic products that render infiltrating T cells insensitive to FASL-induced killing may break the immunologic unresponsiveness to melanoma and provide a complementary approach in the therapy of malignant melanoma. FASL-mediated apoptosis is important for skin homeostasis, the dysregulation of FAS-FASL interactions may be central to the development of skin cancer. Polymorphisms affecting either the expression or biologic activities of FAS or FASL may contribute to the genetic risk of developing T-cell lymphoblastic lymphomas.

Anti-FASL Antibody Images

Click the images to enlarge.

Anti-FASL Antibody
Anti-Fas Ligand Picoband antibody, PB9068-1.JPG
IHC(P): Mouse Intestine Tissue
Anti-FASL Antibody
Anti-Fas Ligand Picoband antibody, PB9068-2.JPG
IHC(P): Rat Intestine Tissue
Anti-FASL Antibody
Anti-Fas Ligand Picoband antibody, PB9068-3.jpg
All lanes: Anti-Fas Ligand(PB9068) at 0.5ug/ml
WB: Rat Thymus Tissue Lysate at 40ug
Predicted bind size: 37KD
Observed bind size: 37KD
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Publications

Yeung CK, Wang G, Yao Y, Liang J, Tenny Chung CY, Chuai M, Lee KK, Yang X. Cell Death Dis. 2017 Mar 23;8(3):e2697. doi: 10.1038/cddis.2017.91. BRE modulates granulosa cell death to affect ovarian follicle development and atresia in the mouse.
Wang Xy, Zhang R, Lian S. Clin Exp Dermatol. 2011 Jan;36(1):69-76. Doi: 10.1111/J.1365-2230.2010.03860.X. Aberrant Expression Of Fas And Fasl Pro-Apoptotic Proteins In Basal Cell And Squamous Cell Carcinomas.
Wang J, Tang T, Yang H, Yao X, Chen L, Liu W, Li T. J Neurosurg Spine. 2007 May;6(5):425-30. The Expression Of Fas Ligand On Normal And Stabbed-Disc Cells In A Rabbit Model Of Intervertebral Disc Degeneration: A Possible Pathogenesis.
Ma H, Zhang X, Zhang X, Yang D, Meng L, Zhang Y, Zhou S. Arch Med Sci. 2013 Apr 20;9(2):354-60. Doi: 10.5114/Aoms.2012.31439. Epub 2012 Oct 30. The Effect Of Esculentoside A On Lupus Nephritis-Prone Bxsb Mice.
Liu Mw, Su Mx, Zhang W, Zhang Lm, Wang Yh, Qian Cy. Int J Mol Med. 2015 Aug;36(2):386-98. Doi: 10.3892/Ijmm.2015.2241. Epub 2015 Jun 10. Rhodiola Rosea Suppresses Thymus T-Lymphocyte Apoptosis By Downregulating Tumor Necrosis Factor-??-Induced Prot...
Li Q, Peng J, Li Xh, Liu T, Liang Qc, Zhang Gy. World J Gastroenterol. 2010 Mar 14;16(10):1274-8. Clinical Significance Of Fas And Fasl Protein Expression In Gastric Carcinoma And Local Lymph Node Tissues.
Han X, Qiu L, Zhang Y, Kong Q, Wang H, Wang H, Li H, Duan C, Wang Y, Song Y, Wang C. Exp Biol Med (Maywood). 2009 May;234(5):595-603. Doi: 10.3181/0812-Rm-359. Epub 2009 Feb 25. Transplantation Of Sertoli-Islet Cell Aggregates Formed By Microgravi...
Wu Yh, Hu Sq, Liu J, Cao Hc, Xu W, Li Yj, Li Lj. Int J Mol Med. 2014 Jun;33(6):1498-506. Doi: 10.3892/Ijmm.2014.1730. Epub 2014 Apr 7. Nature And Mechanisms Of Hepatocyte Apoptosis Induced By D-Galactosamine/Lipopolysaccharide Challenge In Mice.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to fas ligand antibody, fasl antibody