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SKU:PA1853
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-Fatty Acid Binding Protein 5 Antibody
SKU/Catalog Number PA1853
Description Rabbit IgG polyclonal antibody for Fatty acid-binding protein, epidermal(FABP5) detection. Tested with WB, IHC-P in Mouse;Rat.
Cite This Product Anti-Fatty Acid Binding Protein 5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1853)
Replacement Item This antibody may replace the following items: sc-16060|sc-365166|sc-365236|sc-373788|sc-50379 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Mouse, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the N-terminus of mouse Fatty Acid Binding Protein 5(10-23aa KWRLMESHGFEEYM), different from the related rat sequence by two amino acids.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Fabp5
Protein Name Fatty acid-binding protein, epidermal
Molecular Weight 15137 MW
Protein Function High specificity for fatty acids. Highest affinity for C18 chain length (By similarity). .
Tissue Specificity Most abundant in keratinocytes and also in stratified epithelia of epidermis and tongue. Relatively high levels found in adipose and mammary tissues and small amounts found in heart, brain, liver, spleen, muscle and lung.
Subcellular Localization Cytoplasm.
Uniprot ID Q05816
Alternative Names Fatty acid-binding protein, epidermal;Epidermal-type fatty acid-binding protein;E-FABP;Fatty acid-binding protein 5;Keratinocyte lipid-binding protein;Psoriasis-associated fatty acid-binding protein homolog;PA-FABP;Fabp5;Fabpe, Klbp, Mal1;
Research Areas |cardiovascular|lipids / lipoproteins|fatty acids|binding proteins| signal transduction|metabolism|lipid metabolism| cardiovascular| metabolism|pathways and processes|metabolic signaling pathways|lipid and lipoprotein metabolism|types of disease|cancer|redox metabolism|fatty acid oxidation|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Fatty acid-binding protein, epidermal

FABP5(fatty acid binding protein 5(psoriasis-associated)) is a protein that in humans is encoded by the FABP5 gene, also known as PAFABP, EFABP, E-FABP, KFABP, PA-FABP, Epidermal-type fatty acid-binding protein, Fatty acid-binding protein 5, Psoriasis-associated fatty acid-binding protein homolog. It is a family of small, highly conserved, cytoplasmic proteins that bind long-chain fatty acids and other hydrophobic ligands. The PAFABP cDNA encodes a 135-amino acid protein with molecular weight 15,164. This gene encodes the fatty acid binding protein found in epidermal cells, and was first identified as being upregulated in psoriasis tissue. It is though that FABPs roles include fatty acid uptake, transport, and metabolism. Kaczocha et al.(2009) identified FABP5 and FABP7 as cytosolic proteins that transport AEA from the plasma membrane to subcellular fatty acid amide hydrolase, where it is hydrolyzed and inactivated. FABP3 did not show this specific transport function.

Anti-Fatty Acid Binding Protein 5 Antibody Images

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Anti-Fatty Acid Binding Protein 5 Antibody
Anti-Fatty Acid Binding Protein 5 antibody, PA1853, Western blotting
All lanes: Anti Fatty Acid Binding Protein 5 (PA1853) at 0.5ug/ml
Lane 1: Rat Liver Tissue Lysate at 50ug
Lane 2: Rat Kidney Tissue Lysate at 50ug
Predicted bind size: 15KD
Observed bind size: 15KD
Anti-Fatty Acid Binding Protein 5 Antibody
Anti-Fatty Acid Binding Protein 5 antibody, PA1853, IHC(P)
IHC(P): Rat Intestine Tissue
Anti-Fatty Acid Binding Protein 5 Antibody
Anti-Fatty Acid Binding Protein 5 antibody, PA1853, IHC(P)
IHC(P): Mouse Cardiac Muscle
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FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is fabp5 antibody