Product Info Summary
SKU: | A02531 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ICC, WB |
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Product info
Product Name
Anti-Fbx32 FBXO32 Antibody
SKU/Catalog Number
A02531
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Fbx32 FBXO32 Antibody catalog # A02531. Tested in WB,ICC applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Fbx32 FBXO32 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02531)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human SPAK around the phosphorylation site of S325.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02531 is reactive to Fbxo32 in Human, Mouse, Rat
Applications
A02531 is guaranteed for ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
41.504kDa
Background of Fbx32
Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites. May mediate calcium-dependent regulation on many physiological processes by interacting with other proteins, such as TPR-containing proteins, and modulating their activity.
Patrick Most, J. Biol. Chem., Nov 2003; 278: 48404 - 48412.
Miki Okada, J. Biol. Chem., Feb 2004; 279: 4221 - 4233.
Melanie Boerries, Mol. Cell. Biol., Jun 2007; 27: 4365 - 4373.
Patrick Most, J. Clin. Invest., Dec 2004; 114: 1550 - 1563.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50-1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of FBXO32 using Anti-Fbx32 FBXO32 Antibody (A02531).
ICC staining Fbx32 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-Fbx32 FBXO32 Antibody A02531
Western blot analysis of Fbx32 on mouse skeletal muscle tissue using anti-Fbx32 antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For Fbxo32 (Source: Uniprot.org, NCBI)
Gene Name
Fbxo32
Full Name
F-box only protein 32
Weight
41.504kDa
Alternative Names
atrogin 1; ATROGIN1; atrogin-1; F-box only protein 32; F-box protein 32; Fbx32; FLJ32424; MAFBX; MAFbxMGC33610; Muscle atrophy F-box protein Fbxo32|4833442G10Rik, AI430017, ATR, ATROGIN1, Gm20361, MAFb, MAFbx, atro|F-box protein 32|F-box only protein 32|atrogin 1|muscle atrophy F-box protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Fbxo32, check out the Fbxo32 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Fbxo32: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Fbx32 FBXO32 Antibody (A02531)
Hello CJ!
No publications found for A02531
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