Anti-FGF21 Rabbit Monoclonal Antibody

FGF-21 antibody

Boster Bio Anti-FGF21 Rabbit Monoclonal Antibody catalog # M00802. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M00802
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-FGF21 Rabbit Monoclonal Antibody

View all FGF-21 Antibodies

SKU/Catalog Number

M00802

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-FGF21 Rabbit Monoclonal Antibody catalog # M00802. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-FGF21 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00802)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AG-6

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human FGF21

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00802 is reactive to FGF21 in Human, Mouse, Rat

Applications

M00802 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

63 kDa

Calculated molecular weight

22.3kDa

Background of FGF-21

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For FGF21 (Source: Uniprot.org, NCBI)

Gene Name

FGF21

Full Name

Fibroblast growth factor 21

Weight

22.3kDa

Superfamily

heparin-binding growth factors family

Alternative Names

FGF21; FGF-21; fibroblast growth factor 21 FGF21 fibroblast growth factor 21 fibroblast growth factor 21

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on FGF21, check out the FGF21 Infographic

FGF21 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for FGF21: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-FGF21 Rabbit Monoclonal Antibody

Question

I see that the anti-FGF21 Rabbit Monoclonal antibody M00802 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-20

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-20

Question

My boss were content with the WB result of your anti-FGF21 Rabbit Monoclonal antibody. However we have observed positive staining in lung secreted. using this antibody. Is that expected? Could you tell me where is FGF21 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

From literature, lung does express FGF21. Generally FGF21 expresses in secreted. Regarding which tissues have FGF21 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-12-04

Question

We have seen staining in human lung. Do you have any suggestions? Is anti-FGF21 Rabbit Monoclonal antibody supposed to stain lung positively?

Verified Customer

Verified customer

Asked: 2019-10-04

Answer

From literature lung does express FGF21. From Uniprot.org, FGF21 is expressed in right lobe of liver, lung, among other tissues. Regarding which tissues have FGF21 expression, here are a few articles citing expression in various tissues:
Lung, Pubmed ID: 15489334

Boster Scientific Support

Answered: 2019-10-04

Question

Would M00802 anti-FGF21 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

As indicated on the product datasheet, M00802 anti-FGF21 Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-19

Question

Is there a BSA free version of anti-FGF21 Rabbit Monoclonal antibody M00802 available?

Verified Customer

Verified customer

Asked: 2019-08-23

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-FGF21 Rabbit Monoclonal antibody M00802 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-08-23

Question

Would anti-FGF21 Rabbit Monoclonal antibody M00802 work for WB with lung?

Verified Customer

Verified customer

Asked: 2019-07-12

Answer

According to the expression profile of lung, FGF21 is highly expressed in lung. So, it is likely that anti-FGF21 Rabbit Monoclonal antibody M00802 will work for WB with lung.

Boster Scientific Support

Answered: 2019-07-12

Question

We are currently using anti-FGF21 Rabbit Monoclonal antibody M00802 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2019-04-03

Answer

The anti-FGF21 Rabbit Monoclonal antibody (M00802) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-04-03

Question

We bought anti-FGF21 Rabbit Monoclonal antibody for IHC on lung in a previous project. I am using rat, and I plan to use the antibody for WB next. I am looking for examining lung as well as right lobe of liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2017-09-05

Answer

I looked at the website and datasheets of our anti-FGF21 Rabbit Monoclonal antibody and it appears that M00802 has been tested on rat in both IHC and WB. Thus M00802 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2017-09-05

Question

Is a blocking peptide available for product anti-FGF21 Rabbit Monoclonal antibody (M00802)?

J. Thomas

Verified customer

Asked: 2017-03-08

Answer

We do provide the blocking peptide for product anti-FGF21 Rabbit Monoclonal antibody (M00802). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2017-03-08

Question

Can you help my question with product M00802, anti-FGF21 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Singh

Verified customer

Asked: 2016-11-02

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00802 anti-FGF21 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-11-02

Question

I am interested in using your anti-FGF21 Rabbit Monoclonal antibody for negative regulation of endothelial cell apoptotic process studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

H. Lewis

Verified customer

Asked: 2016-08-31

Answer

I appreciate your inquiry. This M00802 anti-FGF21 Rabbit Monoclonal antibody is tested on hela cell lysate. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2016-08-31

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-FGF21 Rabbit Monoclonal antibody M00802. Let me know if you need anything else.

B. Dhar

Verified customer

Asked: 2015-08-19

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-08-19

Question

I am looking for to test anti-FGF21 Rabbit Monoclonal antibody M00802 on mouse lung for research purposes, then I may be interested in using anti-FGF21 Rabbit Monoclonal antibody M00802 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

N. Krishna

Verified customer

Asked: 2014-06-27

Answer

The products we sell, including anti-FGF21 Rabbit Monoclonal antibody M00802, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2014-06-27

Question

Is this M00802 anti-FGF21 Rabbit Monoclonal antibody reactive to the isotypes of FGF21?

K. Parker

Verified customer

Asked: 2014-03-10

Answer

The immunogen of M00802 anti-FGF21 Rabbit Monoclonal antibody is A synthesized peptide derived from human FGF21. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-03-10

Question

Please see the WB image, lot number and protocol we used for lung using anti-FGF21 Rabbit Monoclonal antibody M00802. Please let me know if you require anything else.

C. Parker

Verified customer

Asked: 2013-10-09

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-10-09

Question

I was wanting to use your anti-FGF21 Rabbit Monoclonal antibody for WB for mouse lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse lung identification?

R. Lewis

Verified customer

Asked: 2013-09-12

Answer

It shows on the product datasheet, M00802 anti-FGF21 Rabbit Monoclonal antibody has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse lung in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2013-09-12

Order DetailsPrice
M00802

100μL

$370
M00802-30ul

30μL sample

$99
M00802-carrier-free

Carrier Free

$370

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M00802
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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