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SKU:MA1116
Clonality:Monoclonal
Clone Number:FN-15
Application:IHC-P, IHC-F, ICC, WB
Price: $200.00
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Overview

Product Name Anti-Fibronectin Antibody
Description Mouse IgG monoclonal antibody for Fibronectin, fibronectin 1 (FN1 ) detection. Tested with WB, IHC-P, IHC-F, ICC in Human;mouse;chicken. No cross reactivity with other proteins.
Cite This Product Anti-Fibronectin Antibody FN-15 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1116)
Replacement Item This antibody may replace the following items: sc-18825|sc-18826|sc-18827|sc-271098|sc-50078|sc-50079|sc-50080|sc-50081|sc-52331|sc-53285|sc-53286|sc-59824|sc-59826|sc-6952|sc-6953|sc-69681|sc-69682|sc-73611|sc-81767|sc-81769|sc-8422|sc-9068|sc-98454 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Chicken, Human, Mouse
Application IHC-P, IHC-F, ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P), IHC(F) and ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Fibronectin from human plasma.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number FN-15
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Fn1
Protein Name Fibronectin
Molecular Weight 272511 MW
Protein Function Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts (By similarity). .
Tissue Specificity Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
Sequence Similarities Contains 12 fibronectin type-I domains.
Subcellular Localization Secreted, extracellular space, extracellular matrix.
Uniprot ID P04937
Alternative Names Fibronectin;FN;Anastellin;Fn1;
Research Areas FN1|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Fibronectin

Fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. Cellular fibronectin is a 550KDa glycoprotein existing as two heterodimers, different from plasma fibronectin in the presence of additional polypeptide segments and in altering morphology of transformed cells and hemaglutination.
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Publications

Fu Jr, Liu Wl, Zhou Yf, Zhou Jf, Sun Hy, Luo L, Zhang H, Xu Hz. Chin Med J (Engl). 2005 Dec 5;118(23):1979-86. Expansive Effects Of Aorta-Gonad-Mesonephros-Derived Stromal Cells On Hematopoietic Stem Cells From Embryonic Stem Cells.
Xue H, Zhang Yl, Liu Gs, Wang H. J Pharmacol Exp Ther. 2005 Nov;315(2):501-9. Epub 2005 Jul 28. A New Atp-Sensitive Potassium Channel Opener Protects The Kidney From Hypertensive Damage In Spontaneously Hypertensive Rats.
Yang Y, Gao X, Wang X, Su L, Xing H. Eur J Pharm Sci. 2012 Aug 30;47(1):75-83. Doi: 10.1016/J.Ejps.2012.05.005. Epub 2012 May 23. Total Flavonoids Of Fructus Chorspondiatis Inhibits Collagen Synthesis Of Cultured Rat Cardiac Fibroblasts Induced By...
Guo Y, Zeng Qc, Zhang Cq, Zhang Xz, Li Rx, Wu Jm, Guan J, Liu L, Zhang Xc, Li Jy, Wan Zm. Int J Med Sci. 2013 Nov 5;10(13):1837-45. Doi: 10.7150/Ijms.6786. Ecollection 2013. Extracellular Matrix Of Mechanically Stretched Cardiac Fibroblasts Improv...
Wang H, Wang Hs, Zhou Bh, Li Cl, Zhang F, Wang Xf, Zhang G, Bu Xz, Cai Sh, Du J. Plos One. 2013;8(2):E56664. Doi: 10.1371/Journal.Pone.0056664. Epub 2013 Feb 19. Epithelial-Mesenchymal Transition (Emt) Induced By Tnf-?? Requires Akt/Gsk-3??-Mediated...
Shi Z, Shen T, Liu Y, Huang Y, Jiao J. J Biol Chem. 2014 Mar 7;289(10):6415-28. Doi: 10.1074/Jbc.M113.515601. Epub 2014 Jan 23. Retinoic Acid Receptor ?? (Rarg) And Nuclear Receptor Subfamily 5, Group A, Member 2 (Nr5A2) Promote Conversion Of Fibro...
Gai X, Lu Z, Tu K, Liang Z, Zheng X. Plos One. 2014 Jan 13;9(1):E84551. Doi: 10.1371/Journal.Pone.0084551. Ecollection 2014. Caveolin-1 Is Up-Regulated By Gli1 And Contributes To Gli1-Driven Emt In Hepatocellular Carcinoma.
Xia H, Cheung Wk, Sze J, Lu G, Jiang S, Yao H, Bian Xw, Poon Ws, Kung Hf, Lin Mc. J Biol Chem. 2010 Nov 19;285(47):36995-7004. Doi: 10.1074/Jbc.M110.133744. Epub 2010 Sep 7. Mir-200A Regulates Epithelial-Mesenchymal To Stem-Like Transition Via Zeb...
Cheng Y, Wang Y, Kang Yz, Hu Py, Gao Y, Pan Mx. Digestion. 2013;87(3):189-95. Doi: 10.1159/000349949. Epub 2013 May 1. In Vitro Culture Of Tumour-Derived Hepatocytes In Decellularised Whole-Liver Biological Scaffolds.
Wu Y, Chen W, Guo M, He Q, Hu Y. Int J Clin Exp Pathol. 2014 Jul 15;7(8):4827-36. Ecollection 2014. Effects Of Transforming Growth Factor-??2 On Myocilin Expression And Secretion In Human Primary Cultured Trabecular Meshwork Cells.
Gong X, Liu H, Ding X, Liu M, Li X, Zheng L, Jia X, Zhou G, Zou Y, Li J, Huang X, Fan Y. Biomaterials. 2014 Jun;35(17):4782-91. Doi: 10.1016/J.Biomaterials.2014.02.050. Epub 2014 Mar 15. Physiological Pulsatile Flow Culture Conditions To Generate ...
Xiao H, Ji Am, Li Zl, Song Xd, Su D, Chen Ah. Sheng Li Xue Bao. 2008 Jun 25;60(3):355-61. Interleukin-1?? Inhibits Collagen Synthesis And Promotes Its Decomposition In Cultured Cardiac Fibroblasts.
Zhang H, Teng X, Liu Z, Zhang L, Liu Z. J Exp Clin Cancer Res. 2015 Feb 12;34:16. Doi: 10.1186/S13046-015-0132-Y. Gene Expression Profile Analyze The Molecular Mechanism Of Cxcr7 Regulating Papillary Thyroid Carcinoma Growth And Metastasis.
Zhang W, Chen Dq, Qi F, Wang J, Xiao Wy, Zhu Wz. J Cardiovasc Pharmacol. 2010 Jan;55(1):96-105. Doi: 10.1097/Fjc.0B013E3181C9548B. Inhibition Of Calcium-Calmodulin-Dependent Kinase Ii Suppresses Cardiac Fibroblast Proliferation And Extracellular M...
Sasaki M, Inoue M, Katada Y, Taguchi T. J Mater Sci Mater Med. 2013 Apr;24(4):951-8. Doi: 10.1007/S10856-012-4845-6. Epub 2013 Jan 19. Promotion Of Initial Cell Adhesion On Trisuccinimidyl Citrate-Modified Nickel-Free High-Nitrogen Stainless Steel.

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to fibronectin 1 antibody, fn1 antibody, msf antibody