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SKU:MA1041
Clonality:Monoclonal
Clone Number:FIL-7B10
Application:IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-Filensin Antibody (Monoclonal, FIL-7B10)
Description Mouse IgG monoclonal antibody for Filensin, beaded filament structural protein 1, filensin (BFSP1) detection. Tested with WB, IHC-F in Human;bovine. No cross reactivity with other proteins.
Cite This Product Anti-Filensin Antibody (Monoclonal, FIL-7B10) FIL-7B10 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1041)
Replacement Item This antibody may replace the following items: sc-83024|sc-83022 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Bovine, Human
Application IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human and bovine lens filament enriched fraction(plasma membrane-cytoskeleton complex).
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number FIL-7B10
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Bfsp1
Protein Name Filensin
Molecular Weight 68038 MW
Tissue Specificity Detected in eye lens. .
Sequence Similarities Belongs to the intermediate filament family.
Subcellular Localization Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cytoplasm, cell cortex . Detected adjacent to the cell membrane. .
Uniprot ID Q02435
Alternative Names Filensin;Beaded filament structural protein 1;Lens fiber cell beaded-filament structural protein CP 94;CP94;Bfsp1;
Research Areas |neuroscience|sensory system|visual system|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Filensin

Filensin, also known as beaded filament structure protein 1, have two major component BFSP1 and BESP2. Filensin gene is mapped at 20p12.1-p11.23. The sequence of the predicted 665-amino acid human protein is 62% and 50% identical to those of bovine and chicken filensin, respectively. However, it has less than 26% identity to other members of the intermediate filament(IF) family.
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.