Anti-Flk-1 KDR Antibody

Boster Bio Anti-Flk-1 KDR Antibody catalog # A00901. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: A00901
Size: 100μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Flk-1 KDR Antibody

See all VEGFR2/KDR/Flk-1 products

SKU/Catalog Number

A00901

Size

100μl

Form

Liquid

Description

Boster Bio Anti-Flk-1 KDR Antibody catalog # A00901. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Flk-1 KDR Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00901)

Host

Rabbit

Contents

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Isotype

IgG

Immunogen

Synthesized peptide derived from human Flk-1 around the non-phosphorylation site of Y1214.

Reactive Species

A00901 is reactive to KDR in Human, Mouse, Rat

Applications

A00901 is guaranteed for WB Boster Guarantee

*Blocking peptide can be purchased at $150. Contact us for more information.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

WB, 1:500-1:2000

Validation Images & Assay Conditions

Gene/Protein Information For KDR (Source: Uniprot.Org, NCBI)

Uniprot ID

P35968

Gene ID

3791

Gene Name

KDR

Full Name

Vascular endothelial growth factor receptor 2

Weight

151.527kDa

Superfamily

protein kinase superfamily

Alternative Names

CD309 antigen; CD309; EC 2.7.10; EC 2.7.10.1; Fetal liver kinase 1; fetal liver kinase-1; Flk1; Flk-1; FLK1tyrosine kinase growth factor receptor; KDR; kinase insert domain receptor (a type III receptor tyrosine kinase); Kinase insert domain receptor; KRD1; Ly73; Protein-tyrosine kinase receptor flk-1; soluble VEGFR2; vascular endothelial growth factor receptor 2; VEGF R2; VEGFR; VEGFR2; VEGFR-2 KDR CD309, FLK1, VEGFR, VEGFR2 kinase insert domain receptor vascular endothelial growth factor receptor 2|fetal liver kinase-1|kinase insert domain receptor (a type III receptor tyrosine kinase)|protein-tyrosine kinase receptor Flk-1|soluble VEGFR2|tyrosine kinase growth factor receptor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on KDR, check out the KDR Infographic

KDR infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for KDR: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

A00901 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhu Y, Tuerxun A, Hui Y, Abliz P. Exp Ther Med. 2014 Aug;8(2):647-651. Epub 2014 Jun 12. Effects Of Propranolol And Isoproterenol On Infantile Hemangioma Endothelial Cells In Vitro.

Liu M, Yang S, Zhang D, Shui P, Song S, Yao J, Dai Y, Sun Q. Int J Clin Exp Med. 2014 Nov 15;7(11):3859-69. Ecollection 2014. Fructopyrano-(1

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Find answers in Q&As, reviews.

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15 Customer Q&As for Anti-Flk-1 KDR Antibody

Question

We have observed staining in human lung. Do you have any suggestions? Is anti-Flk-1 antibody supposed to stain lung positively?

Verified Customer

Verified customer

Asked: 2020-05-06

Answer

From what I have seen in literature lung does express KDR. From what I have seen in Uniprot.org, KDR is expressed in lung, umbilical vein, among other tissues. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454

Boster Scientific Support

Answered: 2020-05-06

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for umbilical vein using anti-Flk-1 antibody A00901. Let me know if you need anything else.

S. Li

Verified customer

Asked: 2020-03-06

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-06

Question

Here is the WB image, lot number and protocol we used for umbilical vein using anti-Flk-1 antibody A00901. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-23

Question

I see that the anti-Flk-1 antibody A00901 works with WB, what is the protocol used to produce the result images on the product page?

C. Lewis

Verified customer

Asked: 2019-09-26

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-26

Question

My question regarding product A00901, anti-Flk-1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-06-11

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00901 anti-Flk-1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-06-11

Question

We are currently using anti-Flk-1 antibody A00901 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-05-10

Answer

The anti-Flk-1 antibody (A00901) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-05-10

Question

My question regards using your anti-Flk-1 antibody for vegfa-vegfr2 pathway studies. Has this antibody been tested with western blotting on 293 cells? We would like to see some validation images before ordering.

R. Mitchell

Verified customer

Asked: 2019-01-24

Answer

We appreciate your inquiry. This A00901 anti-Flk-1 antibody is tested on 293 cells. It is guaranteed to work for WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-01-24

Question

I was wanting to use your anti-Flk-1 antibody for WB for mouse umbilical vein on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse umbilical vein identification?

Verified Customer

Verified customer

Asked: 2018-10-15

Answer

As indicated on the product datasheet, A00901 anti-Flk-1 antibody has been tested for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse umbilical vein in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-10-15

Question

Would anti-Flk-1 antibody A00901 work for WB with umbilical vein?

Verified Customer

Verified customer

Asked: 2018-05-17

Answer

According to the expression profile of umbilical vein, KDR is highly expressed in umbilical vein. So, it is likely that anti-Flk-1 antibody A00901 will work for WB with umbilical vein.

Boster Scientific Support

Answered: 2018-05-17

Question

Is this A00901 anti-Flk-1 antibody reactive to the isotypes of KDR?

Verified Customer

Verified customer

Asked: 2018-04-30

Answer

The immunogen of A00901 anti-Flk-1 antibody is Synthesized peptide derived from human Flk-1 around the non-phosphorylation site of Y1214. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2018-04-30

Question

Is a blocking peptide available for product anti-Flk-1 antibody (A00901)?

Verified Customer

Verified customer

Asked: 2018-02-14

Answer

We do provide the blocking peptide for product anti-Flk-1 antibody (A00901). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-02-14

Question

Do you have a BSA free version of anti-Flk-1 antibody A00901 available?

Verified Customer

Verified customer

Asked: 2017-11-13

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Flk-1 antibody A00901 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2017-11-13

Question

Our team were satisfied with the WB result of your anti-Flk-1 antibody. However we have seen positive staining in lung cell junction. endoplasmic using this antibody. Is that expected? Could you tell me where is KDR supposed to be expressed?

T. Wu

Verified customer

Asked: 2016-12-27

Answer

From what I have seen in literature, lung does express KDR. Generally KDR expresses in cell junction. endoplasmic, isoform 1: cell membrane. Regarding which tissues have KDR expression, here are a few articles citing expression in various tissues:
Umbilical vein, Pubmed ID: 15815621, 1656371, 7559454

Boster Scientific Support

Answered: 2016-12-27

Question

Would A00901 anti-Flk-1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Krishna

Verified customer

Asked: 2013-04-16

Answer

You can see on the product datasheet, A00901 anti-Flk-1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-04-16

Question

you antibody to test anti-Flk-1 antibody A00901 on mouse umbilical vein for research purposes, then I may be interested in using anti-Flk-1 antibody A00901 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

K. Johnson

Verified customer

Asked: 2013-03-19

Answer

The products we sell, including anti-Flk-1 antibody A00901, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2013-03-19

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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In stock
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A00901
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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