Anti-Forkhead box protein P3 FOXP3 Antibody

Foxp3 antibody

Boster Bio Anti-Forkhead box protein P3 FOXP3 Antibody catalog # PA1577. Tested in WB applications. This antibody reacts with Human. Cited in 10 publication(s).

Product Info Summary

SKU: PA1577
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Forkhead box protein P3 FOXP3 Antibody

View all Foxp3 Antibodies

SKU/Catalog Number

PA1577

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Forkhead box protein P3 FOXP3 Antibody catalog # PA1577. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Forkhead box protein P3 FOXP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1577)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human FOXP3, different from the related mouse sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1577 is reactive to Foxp3 in Human

Applications

PA1577 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

55 kDa

Calculated molecular weight

47.346kDa

Background of Foxp3

FOXP3 (forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome (specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For Foxp3 (Source: Uniprot.org, NCBI)

Gene Name

Foxp3

Full Name

Forkhead box protein P3

Weight

47.346kDa

Alternative Names

AIID; AIIDMGC141961; human foxp3; mouse foxp3; DIETER; forkhead box P3; Forkhead Box Protein P3; foxp3 ihc; foxp3 paraffin; FoxP3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; Immunodeficiency, Polyendocrinopathy, Enteropathy, X-Linked; IPEX; JM2; MGC141961; MGC141963; PIDX; PIDXMGC141963; SCURFIN; XPID; XPIDpolyendocrinopathy, enteropathy, X-linked Foxp3|JM2, scu, scurfin, sf|forkhead box P3|forkhead box protein P3|scurfy

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Foxp3, check out the Foxp3 Infographic

Foxp3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Foxp3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1577 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

IL-35 improves Treg-mediated immune suppression in atherosclerotic mice

Decreased FOXP3+ and GARP+ Tregs to neoadjuvant chemotherapy associated with favorable prognosis in advanced gastric cancer

LncRNA DQ786243 affects Treg related CREB and Foxp3 expression in Crohn?s disease

Wang B, Dai S, Dong Z, Sun Y, Song X, Guo C, Zhu F, Wang Q, Zhang L. Plos One. 2014 Jan 30;9(1):E87787. Doi: 10.1371/Journal.Pone.0087787. Ecollection 2014. The Modulation Of Endoplasmic Reticulum Stress By Chemical Chaperone Upregulates Immune Ne...

Hu M, Li K, Maskey N, Xu Z, Peng C, Wang B, Li Y, Yang G. Int J Clin Exp Pathol. 2014 Jul 15;7(8):4685-94. Ecollection 2014. Decreased Intratumoral Foxp3 Tregs And Increased Dendritic Cell Density By Neoadjuvant Chemotherapy Associated With Favora...

Gai Xd, Song Y, Li C, Lei Ym, Yang B. Pathol Res Pract. 2013 Dec;209(12):774-8. Doi: 10.1016/J.Prp.2013.08.011. Epub 2013 Sep 6. Potential Role Of Plasmacytoid Dendritic Cells For Foxp3+ Regulatory T Cell Development In Human Colorectal Cancer And...

Gai Xd, Li C, Song Y, Lei Ym, Yang Bx. Biomed Rep. 2013 Mar;1(2):207-212. Epub 2012 Oct 29. In Situ Analysis Of Foxp3+ Regulatory T Cells And Myeloid Dendritic Cells In Human Colorectal Cancer Tissue And Tumor-Draining Lymph Node.

Fu Hy, Li C, Yang W, Gai Xd, Jia T, Lei Ym, Li Y. Acta Histochem. 2013 Mar;115(2):151-7. Doi: 10.1016/J.Acthis.2012.06.002. Epub 2012 Jun 29. Foxp3 And Tlr4 Protein Expression Are Correlated In Non-Small Cell Lung Cancer: Implications For Tumor Pr...

Li G, Ren J, Wang G, Gu G, Hu D, Ren H, Hong Z, Wu X, Liu S, Li J. Int Immunopharmacol. 2014 Feb;18(2):244-8. Doi: 10.1016/J.Intimp.2013.12.014. Epub 2013 Dec 22. T2 Enhances In Situ Level Of Foxp3+ Regulatory Cells And Modulates Inflammatory Cyto...

Gong T, Wang Cf, Yuan Jr, Li Y, Gu Jf, Zhao Bj, Zhang L, Jia Xb, Feng L, Liu Sl. Evid Based Complement Alternat Med. 2015;2015:630760. Doi: 10.1155/2015/630760. Epub 2015 May 3. Inhibition Of Tumor Growth And Immunomodulatory Effects Of Flavonoids...

Have you used Anti-Forkhead box protein P3 FOXP3 Antibody?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Forkhead box protein P3 FOXP3 Antibody

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

2 Customer Q&As for Anti-Forkhead box protein P3 FOXP3 Antibody

Question

I am interested in using your anti-FOXP3 antibody for t cell homeostasis studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-04-07

Answer

We appreciate your inquiry. This PA1577 anti-FOXP3 antibody is tested on hela cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-04-07

Question

We are currently using anti-FOXP3 antibody PA1577 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

The anti-FOXP3 antibody (PA1577) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-03

Order DetailsPrice
PA1577

100μg

$370
PA1577-10ug

10μg sample (liquid)

$99
PA1577-Biotin

100 μg Biotin conjugated

$570
PA1577-Cy3

100 μg Cy3 conjugated

$570
PA1577-Dylight488

100 μg Dylight488 conjugated

$570
PA1577-Dylight550

100 μg Dylight550 conjugated

$570
PA1577-Dylight594

100 μg Dylight594 conjugated

$570
PA1577-FITC

100 μg FITC conjugated

$570
PA1577-HRP

100 μg HRP conjugated

$570
PA1577-APC

100 μg APC conjugated

$670
PA1577-PE

100 μg PE conjugated

$670
PA1577-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
PA1577
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.