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SKU:RP1032
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P
Price: $200.00
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Overview

Product Name Anti-FOXP3 Antibody
SKU/Catalog Number RP1032
Description Rabbit IgG polyclonal antibody for Forkhead box protein P3(FOXP3) detection. Tested with IHC-P in Human;Mouse;Rat.
Cite This Product Anti-FOXP3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1032)
Replacement Item This antibody may replace the following items: sc-31738|sc-21072|sc-31739|sc-28705|sc-53876|sc-130666|sc-166212|sc-65988|sc-52899|sc-31738-X|sc-21072-X|sc-31739-X|sc-28705-X|sc-166212-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human FOXP3 recombinant protein (Position: H101-P431). Human FOXP3 shares 88% amino acid (aa) sequence identity with mouse FOXP3.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name FOXP3
Protein Name Forkhead box protein P3
Molecular Weight 47244 MW
Protein Function Transcriptional regulator which is crucial for the development and inhibitory function of regulatory T-cells (Treg). Plays an essential role in maintaining homeostasis of the immune system by allowing the acquisition of full suppressive function and stability of the Treg lineage, and by directly modulating the expansion and function of conventional T-cells. Can act either as a transcriptional repressor or a transcriptional activator depending on its interactions with other transcription factors, histone acetylases and deacetylases. The suppressive activity of Treg involves the coordinate activation of many genes, including CTLA4 and TNFRSF18 by FOXP3 along with repression of genes encoding cytokines such as interleukin-2 (IL2) and interferon- gamma (IFNG). Inhibits cytokine production and T-cell effector function by repressing the activity of two key transcription factors, RELA and NFATC2 (PubMed:15790681). Mediates transcriptional repression of IL2 via its association with histone acetylase KAT5 and histone deacetylase HDAC7 (PubMed:17360565). Can activate the expression of TNFRSF18, IL2RA and CTLA4 and repress the expression of IL2 and IFNG via its association with transcription factor RUNX1 (PubMed:17377532). Inhibits the differentiation of IL17 producing helper T-cells (Th17) by antagonizing RORC function, leading to down-regulation of IL17 expression, favoring Treg development (PubMed:18368049). Inhibits the transcriptional activator activity of RORA (PubMed:18354202). Can repress the expression of IL2 and IFNG via its association with transcription factor IKZF4 (By similarity). .
Sequence Similarities Contains 1 C2H2-type zinc finger.
Subcellular Localization Nucleus . Cytoplasm . Predominantly expressed in the cytoplasm in activated conventional T-cells whereas predominantly expressed in the nucleus in regulatory T-cells (Treg). The 41 kDa form derived by proteolytic processing is found exclusively in the chromatin fraction of activated Treg cells (By similarity). .
Uniprot ID Q9BZS1
Alternative Names Forkhead box protein P3;Scurfin;Forkhead box protein P3, C-terminally processed;Forkhead box protein P3 41 kDa form;FOXP3;IPEX;JM2;
Research Areas |cell biology|cell cycle|cell division|chromatid cohesion| epigenetics and nuclear signaling|transcription|domain families|forkhead box|foxp| immunology|adaptive immunity|regulatory t cells|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Forkhead box protein P3

FOXP3(forkhead box P3) is a protein involved in immune system responses. The human FOXP3 genes contain 11 coding exons. Exon-intron boundaries are identical across the coding regions of the mouse and human genes. By genomic sequence analysis, the FOXP3 gene maps to the p arm of the X chromosome(specifically, Xp11.23). A member of the FOX protein family, FOXP3 appears to function as a master regulator in the development and function of regulatory T cells. While the precise control mechanism has not yet been established, FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are presumed to exert control via similar DNA binding interactions during transcription.

Anti-FOXP3 Antibody Images

Click the images to enlarge.

Anti-FOXP3 Antibody
Anti-FOXP3 Picoband antibody, RP1032-1.JPG
IHC(P): Human Tonsil Tissue
Anti-FOXP3 Antibody
Anti-FOXP3 Picoband antibody, RP1032-2.JPG
IHC(P): Mouse Spleen Tissue
Anti-FOXP3 Antibody
Anti-FOXP3 Picoband antibody, RP1032-3.JPG
IHC(P):Rat Spleen Tissue
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Publications

Wang B, Dai S, Dong Z, Sun Y, Song X, Guo C, Zhu F, Wang Q, Zhang L. Plos One. 2014 Jan 30;9(1):E87787. Doi: 10.1371/Journal.Pone.0087787. Ecollection 2014. The Modulation Of Endoplasmic Reticulum Stress By Chemical Chaperone Upregulates Immune Ne...
Hu M, Li K, Maskey N, Xu Z, Peng C, Wang B, Li Y, Yang G. Int J Clin Exp Pathol. 2014 Jul 15;7(8):4685-94. Ecollection 2014. Decreased Intratumoral Foxp3 Tregs And Increased Dendritic Cell Density By Neoadjuvant Chemotherapy Associated With Favora...
Gai Xd, Song Y, Li C, Lei Ym, Yang B. Pathol Res Pract. 2013 Dec;209(12):774-8. Doi: 10.1016/J.Prp.2013.08.011. Epub 2013 Sep 6. Potential Role Of Plasmacytoid Dendritic Cells For Foxp3+ Regulatory T Cell Development In Human Colorectal Cancer And...
Gai Xd, Li C, Song Y, Lei Ym, Yang Bx. Biomed Rep. 2013 Mar;1(2):207-212. Epub 2012 Oct 29. In Situ Analysis Of Foxp3+ Regulatory T Cells And Myeloid Dendritic Cells In Human Colorectal Cancer Tissue And Tumor-Draining Lymph Node.
Fu Hy, Li C, Yang W, Gai Xd, Jia T, Lei Ym, Li Y. Acta Histochem. 2013 Mar;115(2):151-7. Doi: 10.1016/J.Acthis.2012.06.002. Epub 2012 Jun 29. Foxp3 And Tlr4 Protein Expression Are Correlated In Non-Small Cell Lung Cancer: Implications For Tumor Pr...
Li G, Ren J, Wang G, Gu G, Hu D, Ren H, Hong Z, Wu X, Liu S, Li J. Int Immunopharmacol. 2014 Feb;18(2):244-8. Doi: 10.1016/J.Intimp.2013.12.014. Epub 2013 Dec 22. T2 Enhances In Situ Level Of Foxp3+ Regulatory Cells And Modulates Inflammatory Cyto...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.