Anti-Fractalkine CX3CL1 Antibody

CX3CL1/Fractalkine antibody

Boster Bio Anti-Fractalkine CX3CL1 Antibody catalog # PA1400. Tested in WB applications. This antibody reacts with Human. Cited in 3 publication(s).

Product Info Summary

SKU: PA1400
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

Product Name

Anti-Fractalkine CX3CL1 Antibody

View all CX3CL1/Fractalkine Antibodies

SKU/Catalog Number

PA1400

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Fractalkine CX3CL1 Antibody catalog # PA1400. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Fractalkine CX3CL1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1400)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human CX3CL1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1400 is reactive to CX3CL1 in Human

Applications

PA1400 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

90-100 kDa

Calculated molecular weight

42.203kDa

Background of CX3CL1/Fractalkine

Chemokine (C-X3-C motif) ligand 1, also known as CX3CL1, is a large cytokine protein. It is said to be the only known member of the CX3C chemokine family. It is also commonly known under the names fractalkine (in humans) and neurotactin (in mice). Its gene is mapped to the long arm of chromosome 8. Being the large protein, it has got 373 amino acids. Imai et al. (1997) identified a 7-transmembrane high-affinity receptor (CX3CR1; 601470) for fractalkine and showed that it mediates both the adhesive and migratory functions of fractalkine.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For CX3CL1 (Source: Uniprot.org, NCBI)

Gene Name

CX3CL1

Full Name

Fractalkine

Weight

42.203kDa

Superfamily

intercrine delta family

Alternative Names

ABCD-3; C3Xkine; chemokine (C-X3-C motif) ligand 1; CX3C membrane-anchored chemokine; CX3CL1; CXC3; CXC3C; FKN; Fractalkine; Neurotactin; neurotactin); NTNsmall inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine; NTTSmall-inducible cytokine D1; SCYD1C-X3-C motif chemokine 1; small inducible cytokine subfamily D (Cys-X3-Cys), member-1 CX3CL1 ABCD-3, C3Xkine, CXC3, CXC3C, NTN, NTT, SCYD1, fractalkine, neurotactin C-X3-C motif chemokine ligand 1 fractalkine|C-X3-C motif chemokine 1|CX3C membrane-anchored chemokine|chemokine (C-X3-C motif) ligand 1|small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin)|small inducible cytokine subfamily D (Cys-X3-Cys), member-1|small-inducible cytokine D1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CX3CL1, check out the CX3CL1 Infographic

CX3CL1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CX3CL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1400 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Distribution Change of Mast Cells in Human Nasal Polyps

Distribution Change of Mast Cells in Human Nasal Polyps

Distribution Change of Mast Cells in Human Nasal Polyps

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3 Customer Q&As for Anti-Fractalkine CX3CL1 Antibody

Question

We are currently using anti-CX3CL1 antibody PA1400 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-04-17

Answer

The anti-CX3CL1 antibody (PA1400) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-04-17

Question

We have been able to see staining in human thoracic mammary gland. What should we do? Is anti-CX3CL1 antibody supposed to stain thoracic mammary gland positively?

Verified Customer

Verified customer

Asked: 2020-03-30

Answer

Based on literature thoracic mammary gland does express CX3CL1. Based on Uniprot.org, CX3CL1 is expressed in thoracic mammary gland, brain, cerebrospinal fluid, among other tissues. Regarding which tissues have CX3CL1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebrospinal fluid, Pubmed ID: 19838169

Boster Scientific Support

Answered: 2020-03-30

Question

My colleagues were happy with the WB result of your anti-CX3CL1 antibody. However we have observed positive staining in thoracic mammary gland cell membrane using this antibody. Is that expected? Could you tell me where is CX3CL1 supposed to be expressed?

C. Krishna

Verified customer

Asked: 2014-11-27

Answer

Based on literature, thoracic mammary gland does express CX3CL1. Generally CX3CL1 expresses in cell membrane. Regarding which tissues have CX3CL1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 15489334
Cerebrospinal fluid, Pubmed ID: 19838169

Boster Scientific Support

Answered: 2014-11-27

Order DetailsPrice
PA1400

100μg

$370
PA1400-10ug

10μg sample (liquid)

$99
PA1400-Biotin

100 μg Biotin conjugated

$570
PA1400-Cy3

100 μg Cy3 conjugated

$570
PA1400-Dylight488

100 μg Dylight488 conjugated

$570
PA1400-Dylight550

100 μg Dylight550 conjugated

$570
PA1400-Dylight594

100 μg Dylight594 conjugated

$570
PA1400-FITC

100 μg FITC conjugated

$570
PA1400-HRP

100 μg HRP conjugated

$570
PA1400-APC

100 μg APC conjugated

$670
PA1400-PE

100 μg PE conjugated

$670
PA1400-carrier-free

Carrier Free

$370

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