|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-FLRG/FSTL3 Antibody|
|Description||Rabbit IgG polyclonal antibody for Follistatin-related protein 3(FSTL3) detection. Tested with WB in Human;Mouse;Rat.|
|Cite This Product||Anti-FLRG/FSTL3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1915)|
|Replacement Item||This antibody may replace the following items: sc-21302|sc-367324 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human FSTL3(145-159aa ECELRAARCRGHPDL), different from the related rat and mouse sequences by one amino acid.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Follistatin-related protein 3|
|Molecular Weight||27663 MW|
|Protein Function||Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 or the nuclear form is probably involved in transcriptional regulation via interaction with MLLT10. .|
|Tissue Specificity||Expressed in a wide range of tissues. .|
|Sequence Similarities||Contains 2 follistatin-like domains.|
|Subcellular Localization||Isoform 1: Secreted.|
|Alternative Names||Follistatin-related protein 3;Follistatin-like protein 3;Follistatin-related gene protein;FSTL3;FLRG;UNQ674/PRO1308;|
|Research Areas|||signal transduction|growth factors/hormones| stem cells|mesenchymal stem cells|osteogenesis|hematopoietic progenitors|lymphoid|b lymphocytic lineage||
Background for Follistatin-related protein 3
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-FLRG/FSTL3 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-FLRG/FSTL3 Antibody Images
Click the images to enlarge.
Recombinant Protein Detection Source: E.coli derived -recombinant Human FSTL3, 39.2KD (162aa tag+ L52-P251)
Lane 1: Recombinant Human FSTL3 Protein 10ng
Lane 2: Recombinant Human FSTL3 Protein 5ng
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,