Product Info Summary
SKU: | PA2235 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Monkey |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-G-protein coupled receptor 30/GPER1 Antibody
View all GPER/GPR30 Antibodies
SKU/Catalog Number
PA2235
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-G-protein coupled receptor 30/GPER1 Antibody catalog # PA2235. Tested in WB applications. This antibody reacts with Human, Monkey.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-G-protein coupled receptor 30/GPER1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2235)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human G-protein coupled receptor 30, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA2235 is reactive to GPER1 in Human, Monkey
Applications
PA2235 is guaranteed for WB Boster Guarantee
Observed Molecular Weight
60 kDa
Calculated molecular weight
42.248kDa
Background of GPER/GPR30
G-protein coupled estrogen receptor 1 (GPER1), also known as the GPR30 or CEPR is a G protein-coupled receptor that in humans is encode d by the GPER gene. This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. It is mapped to 7p22.3. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey
Validation Images & Assay Conditions
Click image to see more details
Anti-G-protein coupled receptor 30 antibody, PA2235, Western blotting
Lane 1: COLO320 Cell Lysate
Lane 2: MCF-7 Cell Lysate
Lane 3: COS7 Cell Lysate
Protein Target Info & Infographic
Gene/Protein Information For GPER1 (Source: Uniprot.org, NCBI)
Gene Name
GPER1
Full Name
G-protein coupled estrogen receptor 1
Weight
42.248kDa
Superfamily
G-protein coupled receptor 1 family
Alternative Names
CEPR; CEPRG-protein coupled receptor 30; Chemoattractant receptor-like 2; chemokine receptor-like 2; CMKRL2; CMKRL2MGC99678; constitutively expressed peptide-like receptor; DRY12; DRY12IL8-related receptor DRY12; FEG-1; FEG-1mER; Flow-induced endothelial G-protein coupled receptor 1; G protein-coupled estrogen receptor 1; G protein-coupled receptor 30; GPCR-BR; GPER; GPR30; GPR30GPCR-Br; G-protein coupled estrogen receptor 1; heptahelix receptor; LERGU; LERGU2; leucine rich protein in GPR30 3'UTR; LyGPR; Lymphocyte-derived G-protein coupled receptor; Membrane estrogen receptor GPER1 CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPER, GPR30, LERGU, LERGU2, LyGPR, mER G protein-coupled estrogen receptor 1 G-protein coupled estrogen receptor 1|G protein-coupled receptor 30|IL8-related receptor DRY12|chemoattractant receptor-like 2|chemokine receptor-like 2|constitutively expressed peptide-like receptor|flow-induced endothelial G-protein coupled receptor 1|heptahelix receptor|lymphocyte-derived G-protein coupled receptor|membrane estrogen receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GPER1, check out the GPER1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPER1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-G-protein coupled receptor 30/GPER1 Antibody (PA2235)
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No publications found for PA2235
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Customer Q&As
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1 Customer Q&As for Anti-G-protein coupled receptor 30/GPER1 Antibody
Question
We are currently using anti-G-protein coupled receptor 30/GPER1 antibody PA2235 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
R. Mitchell
Verified customer
Asked: 2014-04-14
Answer
The anti-G-protein coupled receptor 30/GPER1 antibody (PA2235) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-04-14