Anti-G-protein coupled receptor 30/GPER1 Antibody

GPER/GPR30 antibody

Boster Bio Anti-G-protein coupled receptor 30/GPER1 Antibody catalog # PA2235. Tested in WB applications. This antibody reacts with Human, Monkey.

Product Info Summary

SKU: PA2235
Size: 100 μg/vial
Reactive Species: Human, Monkey
Host: Rabbit
Application: WB

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Product Name

Anti-G-protein coupled receptor 30/GPER1 Antibody

View all GPER/GPR30 Antibodies

SKU/Catalog Number

PA2235

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-G-protein coupled receptor 30/GPER1 Antibody catalog # PA2235. Tested in WB applications. This antibody reacts with Human, Monkey.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-G-protein coupled receptor 30/GPER1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2235)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human G-protein coupled receptor 30, different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA2235 is reactive to GPER1 in Human, Monkey

Applications

PA2235 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

60 kDa

Calculated molecular weight

42.248kDa

Background of GPER/GPR30

G-protein coupled estrogen receptor 1 (GPER1), also known as the GPR30 or CEPR is a G protein-coupled receptor that in humans is encode d by the GPER gene. This gene is a member of the G-protein coupled receptor 1 family and encodes a multi-pass membrane protein that localizes to the endoplasmic reticulum. It is mapped to 7p22.3. The protein binds estrogen, resulting in intracellular calcium mobilization and synthesis of phosphatidylinositol 3,4,5-trisphosphate in the nucleus. This protein therefore plays a role in the rapid nongenomic signaling events widely observed following stimulation of cells and tissues with estrogen. Alternate transcriptional splice variants which encode the same protein have been characterized.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Monkey

Validation Images & Assay Conditions

Gene/Protein Information For GPER1 (Source: Uniprot.org, NCBI)

Gene Name

GPER1

Full Name

G-protein coupled estrogen receptor 1

Weight

42.248kDa

Superfamily

G-protein coupled receptor 1 family

Alternative Names

CEPR; CEPRG-protein coupled receptor 30; Chemoattractant receptor-like 2; chemokine receptor-like 2; CMKRL2; CMKRL2MGC99678; constitutively expressed peptide-like receptor; DRY12; DRY12IL8-related receptor DRY12; FEG-1; FEG-1mER; Flow-induced endothelial G-protein coupled receptor 1; G protein-coupled estrogen receptor 1; G protein-coupled receptor 30; GPCR-BR; GPER; GPR30; GPR30GPCR-Br; G-protein coupled estrogen receptor 1; heptahelix receptor; LERGU; LERGU2; leucine rich protein in GPR30 3'UTR; LyGPR; Lymphocyte-derived G-protein coupled receptor; Membrane estrogen receptor GPER1 CEPR, CMKRL2, DRY12, FEG-1, GPCR-Br, GPER, GPR30, LERGU, LERGU2, LyGPR, mER G protein-coupled estrogen receptor 1 G-protein coupled estrogen receptor 1|G protein-coupled receptor 30|IL8-related receptor DRY12|chemoattractant receptor-like 2|chemokine receptor-like 2|constitutively expressed peptide-like receptor|flow-induced endothelial G-protein coupled receptor 1|heptahelix receptor|lymphocyte-derived G-protein coupled receptor|membrane estrogen receptor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GPER1, check out the GPER1 Infographic

GPER1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GPER1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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1 Customer Q&As for Anti-G-protein coupled receptor 30/GPER1 Antibody

Question

We are currently using anti-G-protein coupled receptor 30/GPER1 antibody PA2235 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?

R. Mitchell

Verified customer

Asked: 2014-04-14

Answer

The anti-G-protein coupled receptor 30/GPER1 antibody (PA2235) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2014-04-14

Order DetailsPrice
PA2235

100μg

$370
PA2235-10ug

10μg sample (liquid)

$99
PA2235-Biotin

100 μg Biotin conjugated

$570
PA2235-Cy3

100 μg Cy3 conjugated

$570
PA2235-Dylight488

100 μg Dylight488 conjugated

$570
PA2235-Dylight550

100 μg Dylight550 conjugated

$570
PA2235-Dylight594

100 μg Dylight594 conjugated

$570
PA2235-FITC

100 μg FITC conjugated

$570
PA2235-HRP

100 μg HRP conjugated

$570
PA2235-APC

100 μg APC conjugated

$670
PA2235-PE

100 μg PE conjugated

$670
PA2235-carrier-free

Carrier Free

$370

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PA2235
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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