|Sample Size:||30ug for $99, contact us for details|
Data & Images
|Product Name||Anti-GABA A Receptor Alpha 1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Gamma-aminobutyric acid receptor subunit alpha-1(GABRA1) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-GABA A Receptor Alpha 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1578)|
|Replacement Item||This antibody may replace the following items: sc-14005|sc-31403|sc-31404|sc-31405|sc-376282|sc-7348|sc-7349|sc-7352|sc-7356 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human GABA A Receptor alpha 1(440-456aa ATYLNREPQLKAPTPHQ), identical to the related rat and mouse sequences.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Gamma-aminobutyric acid receptor subunit alpha-1|
|Molecular Weight||51802 MW|
|Protein Function||Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand- gated chloride channel (By similarity). .|
|Sequence Similarities||Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA1 sub-subfamily.|
|Subcellular Localization||Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.|
|Alternative Names||Gamma-aminobutyric acid receptor subunit alpha-1;GABA(A) receptor subunit alpha-1;GABRA1;|
|Research Areas|||neuroscience|neurotransmitter|amino acids|gaba| neuroscience|neurotransmission|receptors / channels|ligand-gated ion channels|gaba receptors||
Background for Gamma-aminobutyric acid receptor subunit alpha-1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-GABA A Receptor Alpha 1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-GABA A Receptor Alpha 1 Antibody Images
Click the images to enlarge.
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
IHC(P): Rat Brain Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,