Anti-Phospho-GABAB R1 (Ser923) Antibody
|Product Name||Anti-Phospho-GABAB R1 (Ser923) Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Phospho-specific Rabbit Anti-GABAB R1 (Ser923) Antibody tested for IHC, WB in mouse, rat.|
|Cite This Product||Anti-Phospho-GABAB R1 (Ser923) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P07297)|
|Contents/Buffer||100 μl in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50% glycerol.|
|Immunogen||The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser923 of GABAB R1.|
Assay Dilutions Overview
WB, 1:1, 000
Images And Assay Conditions
Western blot of rat synaptic membrane lysate showing specific immunolabeling of the ~102 kDa GABAB R1 protein phosphorylated at Ser923 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by blot treatment with lambda phosphatase (gamma-Ptase, 1200 units for 30 min).
Protein Target Info (Source: Uniprot.org)
|Protein Name||Gamma-aminobutyric acid type B receptor subunit 1|
|Tissue Specificity||Isoform 1A, isoform 1B and isoform 1C are expressed in testis, stomach, spinal cord and brain including cerebral cortical layers, pyramidal cell layers of the hippocampus, granular cell layers of the dentate gyrus, basal ganglia, cerebellum (predominantly in Purkinje cells followed by granular layer). Isoform 1B is also expressed in kidney and liver. Isoform 1D is expressed in forebrain, cerebellum, eye, kidney, and urinary bladder. .|
|Alternative Names||Gamma-aminobutyric acid type B receptor subunit 1;GABA-B receptor 1;GABA-B-R1;GABA-BR1;GABABR1;Gb1;Gabbr1;|
|Subcellular Localization||Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell projection, dendrite. Coexpression of GABBR1 and GABBR2 is required for GABBR1 maturation and transport to the plasma membrane. Colocalizes with ATF4 in hippocampal neuron dendritic membranes.|
|Molecular Weight||111534 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception. .|
|Research Areas||Mouse, Rat
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