Anti-GAD67/GAD1 Antibody Picoband™

GAD1 antibody

Boster Bio Anti-GAD67/GAD1 Antibody Picoband™ catalog # PB9183. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. Cited in 2 publication(s).

Product Info Summary

SKU: PB9183
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-GAD67/GAD1 Antibody Picoband™

View all GAD1 Antibodies

SKU/Catalog Number

PB9183

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GAD67/GAD1 Antibody Picoband™ catalog # PB9183. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GAD67/GAD1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9183)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human GAD67 recombinant protein (Position: N14-D122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9183 is reactive to GAD1 in Human, Mouse, Rat

Applications

PB9183 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

67 kDa

Calculated molecular weight

66.897kDa

Background of GAD1

Glutamate decarboxylase 1 (brain, 67kDa) (GAD67), also known as GAD1, is a human gene. It is mapped to 2q31.1. This gene encodes one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome. Deficiency in this enzyme has been shown to lead to pyridoxine dependency with seizures. Alternative splicing of this gene results in two products, the predominant 67-kD form and a less-frequent 25-kD form.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For GAD1 (Source: Uniprot.org, NCBI)

Gene Name

GAD1

Full Name

Glutamate decarboxylase 1

Weight

66.897kDa

Superfamily

group II decarboxylase family

Alternative Names

67kD); EC 4.1.1; FLJ45882; GAD1; GAD25; GAD67; glutamate decarboxylase 1 (brain, 67kDa); Glutamate decarboxylase 67 kDa isoform GAD1 CPSQ1, DEE89, GAD, SCP glutamate decarboxylase 1 glutamate decarboxylase 1|67 kDa glutamic acid decarboxylase|GAD-67|glutamate decarboxylase 1 (brain, 67kDa)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GAD1, check out the GAD1 Infographic

GAD1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GAD1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9183 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Excitatory and inhibitory neurons in the hippocampus exhibit molecularly distinct large dense core vesicles

Dietary Restriction Affects Neuronal Response Property and GABA Synthesis in the Primary Visual Cortex

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4 Customer Q&As for Anti-GAD67/GAD1 Antibody Picoband™

Question

We are currently using anti-GAD67/GAD1 antibody PB9183 for mouse tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-23

Answer

The anti-GAD67/GAD1 antibody (PB9183) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-23

Question

I see that the anti-GAD67/GAD1 antibody PB9183 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-11-11

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-11-11

Question

My question regarding product PB9183, anti-GAD67/GAD1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-07-15

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9183 anti-GAD67/GAD1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-07-15

Question

Is this PB9183 anti-GAD67/GAD1 antibody reactive to the isotypes of GAD1?

Verified Customer

Verified customer

Asked: 2017-12-22

Answer

The immunogen of PB9183 anti-GAD67/GAD1 antibody is E.coli-derived human GAD67 recombinant protein (Position: N14-D122). Human GAD67 shares 95% amino acid (aa) sequence identity with both mouse and rat GAD67. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-12-22

Order DetailsPrice
PB9183

100μg

$370
PB9183-10ug

10μg sample (liquid)

$99
PB9183-Biotin

100 μg Biotin conjugated

$570
PB9183-Cy3

100 μg Cy3 conjugated

$570
PB9183-Dylight488

100 μg Dylight488 conjugated

$570
PB9183-Dylight550

100 μg Dylight550 conjugated

$570
PB9183-Dylight594

100 μg Dylight594 conjugated

$570
PB9183-FITC

100 μg FITC conjugated

$570
PB9183-HRP

100 μg HRP conjugated

$570
PB9183-APC

100 μg APC conjugated

$670
PB9183-PE

100 μg PE conjugated

$670
PB9183-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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