Anti-Cyclin-G-associated kinase GAK Antibody

GAK antibody

Boster Bio Anti-Cyclin-G-associated kinase GAK Antibody (Catalog # A04246). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A04246
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-Cyclin-G-associated kinase GAK Antibody

View all GAK Antibodies

SKU/Catalog Number

A04246

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-Cyclin-G-associated kinase GAK Antibody (Catalog # A04246). Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Cyclin-G-associated kinase GAK Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04246)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from Internal of human GAK.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A04246 is reactive to GAK in Human, Mouse

Applications

A04246 is guaranteed for IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

143.191kDa

Background of GAK

Associates with cyclin G and CDK5. Seems to act as an auxilin homolog that is involved in the uncoating of clathrin-coated vesicles by Hsc70 in non-neuronal cells. Expression oscillates slightly during the cell cycle, peaking at G1.

Kimura S.H., Genomics 44:179-187(1997).
The MGC Project Team; Genome Res. 14:2121-2127(2004).
Greener T., J. Biol. Chem. 275:1365-1370(2000).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For GAK (Source: Uniprot.org, NCBI)

Gene Name

GAK

Full Name

Cyclin-G-associated kinase

Weight

143.191kDa

Superfamily

protein kinase superfamily

Alternative Names

Auxillin 2; cyclin G associated kinase; cyclin-G-associated kinase; EC 2.7.11.1; FLJ16629; FLJ40395; GAK; MGC99654 GAK DNAJ26, DNAJC26 cyclin G associated kinase cyclin-G-associated kinase|auxilin-2

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GAK, check out the GAK Infographic

GAK infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GAK: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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13 Customer Q&As for Anti-Cyclin-G-associated kinase GAK Antibody

Question

Will anti-GAK antibody A04246 work for WB with fibroblast?

Verified Customer

Verified customer

Asked: 2020-05-08

Answer

According to the expression profile of fibroblast, GAK is highly expressed in fibroblast. So, it is likely that anti-GAK antibody A04246 will work for WB with fibroblast.

Boster Scientific Support

Answered: 2020-05-08

Question

My question regarding product A04246, anti-GAK antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04246 anti-GAK antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2020-04-23

Question

Does anti-GAK antibody A04246 work on horse WB with endothelial cell?

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

Our lab technicians have not tested anti-GAK antibody A04246 on horse. You can run a BLAST between horse and the immunogen sequence of anti-GAK antibody A04246 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse endothelial cell in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-01-02

Question

I would like to test anti-GAK antibody A04246 on human fibroblast for research purposes, then I may be interested in using anti-GAK antibody A04246 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-12-04

Answer

The products we sell, including anti-GAK antibody A04246, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-12-04

Question

We are currently using anti-GAK antibody A04246 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-08

Answer

The anti-GAK antibody (A04246) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-08

Question

Is a blocking peptide available for product anti-GAK antibody (A04246)?

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

We do provide the blocking peptide for product anti-GAK antibody (A04246). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-09-30

Question

Is there a BSA free version of anti-GAK antibody A04246 available?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GAK antibody A04246 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-19

Question

Would A04246 anti-GAK antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-08-22

Answer

It shows on the product datasheet, A04246 anti-GAK antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-08-22

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fibroblast using anti-GAK antibody A04246. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-19

Question

Here is the WB image, lot number and protocol we used for fibroblast using anti-GAK antibody A04246. Please let me know if you require anything else.

O. Krishna

Verified customer

Asked: 2019-08-05

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-08-05

Question

I see that the anti-GAK antibody A04246 works with WB, what is the protocol used to produce the result images on the product page?

D. Jones

Verified customer

Asked: 2019-07-10

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-07-10

Question

I was wanting to use your anti-GAK antibody for WB for human fibroblast on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human fibroblast identification?

Verified Customer

Verified customer

Asked: 2018-03-28

Answer

You can see on the product datasheet, A04246 anti-GAK antibody has been validated for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human fibroblast in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-03-28

Question

Is this A04246 anti-GAK antibody reactive to the isotypes of GAK?

R. Banerjee

Verified customer

Asked: 2017-01-23

Answer

The immunogen of A04246 anti-GAK antibody is Synthesized peptide derived from Internal of human GAK. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-01-23

Order DetailsPrice
A04246-100uL

100uL

$360

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A04246
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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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