Anti-Galectin 1/LGALS1 Antibody

Galectin-1 antibody

Boster Bio Anti-Galectin 1/LGALS1 Antibody catalog # PA1422. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PA1422
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, IHC-F, ICC, WB

Product Name

Anti-Galectin 1/LGALS1 Antibody

View all Galectin-1 Antibodies

SKU/Catalog Number

PA1422

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Galectin 1/LGALS1 Antibody catalog # PA1422. Tested in Flow Cytometry, IF, IHC, IHC-F, ICC, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Galectin 1/LGALS1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1422)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Galectin 1, different from the related rat and mouse sequences by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1422 is reactive to LGALS1 in Human, Mouse, Rat

Applications

PA1422 is guaranteed for Flow Cytometry, IF, IHC, IHC-F, ICC, WB Boster Guarantee

Observed Molecular Weight

15 kDa

Calculated molecular weight

14.716kDa

Background of Galectin-1

Galectin-1 is a protein that in humans is encoded by the LGALS1 gene. Galectins belong to a family of related beta-galactoside-binding lectins, also referred to as S-type or S-Lac lectins. Its gene is mapped to 22q12-q13. Galectin-1 was expressed in a subset of slowly dividing subventricular zone astrocytes, which included the neural stem cells. It has been considered as an autocrine negative growth factor that regulates cell proliferation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For LGALS1 (Source: Uniprot.org, NCBI)

Gene Name

LGALS1

Full Name

Galectin-1

Weight

14.716kDa

Alternative Names

14 kDa laminin-binding protein; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; BHL; DKFZp686E23103; GAL1; gal-1; Galaptin; galectin 1; Galectin1; Galectin-1; GBP; HBL; HLBP14; HPL; L-14; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; lectin, galactoside-binding, soluble, 1; LGALS1; Putative MAPK-activating protein PM12; S-Lac lectin 1 LGALS1 GAL1, GBP galectin 1 galectin-1|14 kDa laminin-binding protein|14 kDa lectin|HBL|HLBP14|HPL|S-Lac lectin 1|beta-galactoside-binding lectin L-14-I|beta-galactoside-binding protein 14kDa|epididymis secretory sperm binding protein|gal-1|galaptin|lactose-binding lectin 1|lectin, galactoside-binding, soluble, 1|putative MAPK-activating protein PM12

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on LGALS1, check out the LGALS1 Infographic

LGALS1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for LGALS1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-Galectin 1/LGALS1 Antibody

Question

We want to test anti-Galectin 1/LGALS1 antibody PA1422 on human placenta promyelocytic leukemia for research purposes, then I may be interested in using anti-Galectin 1/LGALS1 antibody PA1422 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-04-01

Answer

The products we sell, including anti-Galectin 1/LGALS1 antibody PA1422, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-04-01

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for placenta promyelocytic leukemia using anti-Galectin 1/LGALS1 antibody PA1422. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2020-03-02

Answer

Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-03-02

Question

We are currently using anti-Galectin 1/LGALS1 antibody PA1422 for human tissue, and we are satisfied with the IHC results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on monkey tissues as well?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

The anti-Galectin 1/LGALS1 antibody (PA1422) has not been tested for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2020-02-04

Question

Is a blocking peptide available for product anti-Galectin 1/LGALS1 antibody (PA1422)?

Verified Customer

Verified customer

Asked: 2020-01-27

Answer

We do provide the blocking peptide for product anti-Galectin 1/LGALS1 antibody (PA1422). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2020-01-27

Question

We ordered your anti-Galectin 1/LGALS1 antibody for IHC on hepatoma last year. I am using human, and We intend to use the antibody for WB next. Our lab want to know about examining hepatoma as well as lung in our next experiment. Do you have any suggestion on which antibody would work the best for WB?

Verified Customer

Verified customer

Asked: 2019-12-09

Answer

I viewed the website and datasheets of our anti-Galectin 1/LGALS1 antibody and I see that PA1422 has been tested on human in both IHC and WB. Thus PA1422 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-12-09

Question

Our lab want to know about using your anti-Galectin 1/LGALS1 antibody for post-translational protein phosphorylation studies. Has this antibody been tested with western blotting on intestinal cancer tissue? We would like to see some validation images before ordering.

A. Zhang

Verified customer

Asked: 2019-10-03

Answer

Thanks for your inquiry. This PA1422 anti-Galectin 1/LGALS1 antibody is validated on intestinal cancer tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2019-10-03

Question

Would PA1422 anti-Galectin 1/LGALS1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-07-25

Answer

As indicated on the product datasheet, PA1422 anti-Galectin 1/LGALS1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-07-25

Question

Please see the WB image, lot number and protocol we used for placenta promyelocytic leukemia using anti-Galectin 1/LGALS1 antibody PA1422. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-05-29

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-05-29

Question

I see that the anti-Galectin 1/LGALS1 antibody PA1422 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-05-08

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-05-08

Question

Does anti-Galectin 1/LGALS1 antibody PA1422 work for WB with placenta promyelocytic leukemia?

O. Zhao

Verified customer

Asked: 2017-06-26

Answer

According to the expression profile of placenta promyelocytic leukemia, LGALS1 is highly expressed in placenta promyelocytic leukemia. So, it is likely that anti-Galectin 1/LGALS1 antibody PA1422 will work for WB with placenta promyelocytic leukemia.

Boster Scientific Support

Answered: 2017-06-26

Question

Our lab were satisfied with the WB result of your anti-Galectin 1/LGALS1 antibody. However we have observed positive staining in thalamus extracellular using this antibody. Is that expected? Could you tell me where is LGALS1 supposed to be expressed?

P. Johnson

Verified customer

Asked: 2017-01-05

Answer

According to literature, thalamus does express LGALS1. Generally LGALS1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2017-01-05

Question

Can you help my question with product PA1422, anti-Galectin 1/LGALS1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

K. Taylor

Verified customer

Asked: 2016-11-02

Answer

We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1422 anti-Galectin 1/LGALS1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-11-02

Question

Do you have a BSA free version of anti-Galectin 1/LGALS1 antibody PA1422 available?

W. Huang

Verified customer

Asked: 2015-01-15

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Galectin 1/LGALS1 antibody PA1422 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2015-01-15

Question

I was wanting to use your anti-Galectin 1/LGALS1 antibody for WB for human placenta promyelocytic leukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human placenta promyelocytic leukemia identification?

C. Miller

Verified customer

Asked: 2014-03-27

Answer

You can see on the product datasheet, PA1422 anti-Galectin 1/LGALS1 antibody has been validated for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human placenta promyelocytic leukemia in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2014-03-27

Question

We have seen staining in human brain. Do you have any suggestions? Is anti-Galectin 1/LGALS1 antibody supposed to stain brain positively?

R. Moore

Verified customer

Asked: 2013-09-10

Answer

From what I have seen in literature brain does express LGALS1. From what I have seen in Uniprot.org, LGALS1 is expressed in adipose tissue, lung, placenta promyelocytic leukemia, hepatoma, placenta, lymphocyte, lung fibroblast, thalamus, placenta skin, brain, platelet, brain, cajal-retzius cell fetal brain cortex, melanoma, spleen, mammary cancer, liver, among other tissues. Regarding which tissues have LGALS1 expression, here are a few articles citing expression in various tissues:
Hepatoma, Pubmed ID: 2719646
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 2719964
Lung fibroblast, Pubmed ID: 12761501
Lymphocyte, Pubmed ID: 1988031
Mammary cancer, Pubmed ID: 19497882
Melanoma, Pubmed ID: 1386213
Placenta, Pubmed ID: 3065332, 3611046, 10369126, 18824694
Placenta, and Promyelocytic leukemia, Pubmed ID: 2910856
Placenta, and Skin, Pubmed ID: 15489334
Platelet, Pubmed ID: 12665801
Spleen, Pubmed ID: 2383549
Thalamus, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2013-09-10

Question

Is this PA1422 anti-Galectin 1/LGALS1 antibody reactive to the isotypes of LGALS1?

V. Johnson

Verified customer

Asked: 2013-04-22

Answer

The immunogen of PA1422 anti-Galectin 1/LGALS1 antibody is A synthetic peptide corresponding to a sequence at the C-terminus of human Galectin 1(119-135aa NYMAADGDFKIKCVAFD), different from the related rat and mouse sequences by one amino acid. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2013-04-22

Order DetailsPrice
PA1422

100μg

$370
PA1422-10ug

10μg sample (liquid)

$99
PA1422-Biotin

100 μg Biotin conjugated

$570
PA1422-Cy3

100 μg Cy3 conjugated

$570
PA1422-Dylight488

100 μg Dylight488 conjugated

$570
PA1422-Dylight550

100 μg Dylight550 conjugated

$570
PA1422-Dylight594

100 μg Dylight594 conjugated

$570
PA1422-FITC

100 μg FITC conjugated

$570
PA1422-HRP

100 μg HRP conjugated

$570
PA1422-APC

100 μg APC conjugated

$670
PA1422-PE

100 μg PE conjugated

$670
PA1422-carrier-free

Carrier Free

$370

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