Data & Images
|Product Name||Anti-Galectin-3/LGALS3 Antibody|
|Description||Rabbit IgG polyclonal antibody for Galectin-3(LGALS3) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-Galectin-3/LGALS3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9081)|
|Replacement Item||This antibody may replace the following items: sc-19280|sc-19283|sc-20157|sc-23938|sc-25279|sc-32790|sc-374253|sc-53127|sc-81728 from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human Galectin 3 recombinant protein (Position: K139-I250). Human Galectin 3 shares 88% and 84% amino acid (aa) sequences identity with mouse and rat Galectin 3, respectively.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||26152 MW|
|Protein Function||Galactose-specific lectin which binds IgE. May mediate with the alpha-3, beta-1 integrin the stimulation by CSPG4 of endothelial cells migration. Together with DMBT1, required for terminal differentiation of columnar epithelial cells during early embryogenesis (By similarity). In the nucleus: acts as a pre-mRNA splicing factor. Involved in acute inflammatory responses including neutrophil activation and adhesion, chemoattraction of monocytes macrophages, opsonization of apoptotic neutrophils, and activation of mast cells. .|
|Tissue Specificity||A major expression is found in the colonic epithelium. It is also abundant in the activated macrophages. Expressed in fetal membranes. .|
|Sequence Similarities||Contains 1 galectin domain.|
|Subcellular Localization||Cytoplasm. Nucleus. Secreted. Secreted by a non-classical secretory pathway and associates with the cell surface.|
|Alternative Names||Galectin-3;Gal-3;35 kDa lectin;Carbohydrate-binding protein 35;CBP 35;Galactose-specific lectin 3;Galactoside-binding protein;GALBP;IgE-binding protein;L-31;Laminin-binding protein;Lectin L-29;Mac-2 antigen;LGALS3;MAC2;|
|Research Areas|||cancer|cell cycle|cell division| cancer|tumor biomarkers|oncoproteins||
Background for Galectin-3
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Galectin-3/LGALS3 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Galectin-3/LGALS3 Antibody Images
Click the images to enlarge.
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Placenta Tissue
All lanes: Anti-Galectin-3(PB9081) at 0.5ug/ml
Lane 1: Hela Whole Cell Lysate at 40ug
Lane 2: SGC Whole Cell Lysate at 40ug
Lane 3: MCF-7 Whole Cell Lysate at 40ug
Lane 4: SW620 Whole Cell Lysate at 40ug
Lane 5: A375 Whole Cell Lysate at 40ug
Lane 6: Smmc Whole Cell Lysate at 40ug
Predicted bind size: 26KD
Observed bind size: 26KD
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,