|Product Name||Anti-gamma Catenin/JUP Antibody|
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Rabbit IgG polyclonal antibody for Junction plakoglobin(JUP) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-gamma Catenin/JUP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1117)|
|Contents/Buffer||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human gamma Catenin(8-22aa EQPIKVTEWQQTYTY), identical to the related mouse and rat sequence.|
|Reactivity||Human, Mouse, Rat|
Assay Dilutions Overview
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
Boster's Secondary Antibodies And IHC, WB Kits
The following reagents are used to generate the images below.Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
Images And Assay Conditions
Anti-gamma Catenin antibody, PA1117, Western blotting
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Anti-gamma Catenin antibody, PA1117, IHC(P)
IHC(P): Human Mammary Cancer Tissue
Protein Target Info (Source: Uniprot.org)
|Protein Name||Junction plakoglobin|
|Alternative Names||Junction plakoglobin;Catenin gamma;Desmoplakin III;Desmoplakin-3;JUP;CTNNG, DP3;|
|Subcellular Localization||Cell junction, adherens junction . Cell junction, desmosome . Cytoplasm, cytoskeleton . Membrane ; Peripheral membrane protein . Cytoplasmic in a soluble and membrane-associated form.|
|Molecular Weight||81745 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). .|
|Research Areas||Actin Etc, Cytoskeleton, Cytoskeleton / Ecm, Microfilaments, Signal Transduction
*You can search these to find other products in these research areas.
|Background||Cateningamma, also known as junction plakoglobin(JUP) or plakoglobin(PKGB). Plakoglobin is a major cytoplasmic protein which is the only known constituent common to submembranous plaques of both desmosomes and intermediate junctions. Cateninbeta and cateningamma(plakoglobin), vertebrate homologs of Drosophila armadillo, function in cell adhesion and the Wnt signaling pathway. Cateningamma may have distinct roles in Wnt signaling and cancer via differential effects on downstream target genes.|
Other Recommended Resources
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1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,