|Validated Species:||Human, Mouse, Rat|
Data & Images
|Product Name||Anti-Gamma Catenin Antibody|
|Description||Rabbit IgG polyclonal antibody for Junction plakoglobin(JUP) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-Gamma Catenin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1117)|
|Replacement Item||This antibody may replace the following items: sc-161270|sc-161268|sc-161267|sc-10117|sc-25575|sc-28359|sc-48402|sc-84618|sc-84619 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the N-terminus of human gamma Catenin(8-22aa EQPIKVTEWQQTYTY), identical to the related mouse and rat sequence.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Junction plakoglobin|
|Molecular Weight||81745 MW|
|Protein Function||Common junctional plaque protein. The membrane- associated plaques are architectural elements in an important strategic position to influence the arrangement and function of both the cytoskeleton and the cells within the tissue. The presence of plakoglobin in both the desmosomes and in the intermediate junctions suggests that it plays a central role in the structure and function of submembranous plaques. Acts as a substrate for VE-PTP and is required by it to stimulate VE- cadherin function in endothelial cells. Can replace beta-catenin in E-cadherin/catenin adhesion complexes which are proposed to couple cadherins to the actin cytoskeleton (By similarity). .|
|Sequence Similarities||Belongs to the beta-catenin family.|
|Subcellular Localization||Cell junction, adherens junction . Cell junction, desmosome . Cytoplasm, cytoskeleton . Membrane ; Peripheral membrane protein . Cytoplasmic in a soluble and membrane-associated form.|
|Alternative Names||Junction plakoglobin;Catenin gamma;Desmoplakin III;Desmoplakin-3;JUP;CTNNG, DP3;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|catenins||
Background for Junction plakoglobin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Gamma Catenin Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Rat, Human, Mouse, By Heat|
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Gamma Catenin Antibody Images
Click the images to enlarge.
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
IHC(P): Human Mammary Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,