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SKU:MA1114
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:GTU-88
Application:ICC, WB
Price: $200.00
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Overview

Product Name Anti-Gamma-Tubulin Antibody (Monoclonal, GTU-88)
SKU/Catalog Number MA1114
Description Mouse IgG monoclonal antibody for gamma-Tubulin, golgi-associated, gamma adaptin ear containing, ARF binding protein 1; golgi-associated, gamma adaptin ear containing, ARF binding protein 2; golgi-associated, gamma adaptin ear containing, ARF binding protein 3 (GGA1; GGA2; GGA3 ) detection. Tested with WB, ICC in Human,mouse,rat. No cross reactivity with other proteins.
Cite This Product Anti-Gamma-Tubulin Antibody (Monoclonal, GTU-88) GTU-88 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1114)
Replacement Item This antibody may replace the following items: sc-130768|sc-133147|sc-135922|sc-135923|sc-19324|sc-19326|sc-23261|sc-23262|sc-23264|sc-23265|sc-271927|sc-30102|sc-30103|sc-30104|sc-390420|sc-390837 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Mouse, Rat
Predicted Species Hamster

*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.

Application ICC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for ICC.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Synthetic gamma-tubulin peptide(amino acids 38-53), conjugated to KLH.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number GTU-88
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Gga3
Protein Name ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation factor-binding protein GGA3
Molecular Weight 71715 MW
Protein Function GGA1: Plays a role in protein sorting and trafficking betweenthe trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and bindsubiquitinated proteins and membrane cargo molecules with acytosolic acidic cluster-dileucine (AC-LL) motif.

GGA2: Plays a role in protein sorting and trafficking betweenthe trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and bindsubiquitinated proteins and membrane cargo molecules with acytosolic acidic cluster-dileucine (AC-LL) motif.

GGA3: Plays a role in protein sorting and trafficking betweenthe trans-Golgi network (TGN) and endosomes. Mediates the ARF-dependent recruitment of clathrin to the TGN and bindsubiquitinated proteins and membrane cargo molecules with acytosolic acidic cluster-dileucine (AC-LL) motif.
Tissue Specificity GGA1: Ubiquitously expressed.
Sequence Similarities Belongs to the GGA protein family.
Subcellular Localization GGA1: Golgi apparatus, trans-Golgi networkmembrane; Peripheral membrane protein. Endosome membrane;Peripheral membrane protein.
Gga1: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane
GGA2: Golgi apparatus, trans-Golgi networkmembrane
Gga2: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane
GGA3: Golgi apparatus, trans-Golgi networkmembrane
Gga3: Golgi apparatus, trans-Golgi networkmembrane Endosome membrane
Uniprot ID D3ZHG8
Alternative Names Golgi associated, gamma adaptin ear containing, ARF binding protein 3 (Predicted) ;Protein Gga3 ;Gga3 ;Gga3_predicted ;rCG_32821 ;
Research Areas signal transduction|protein trafficking|golgi proteins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for ADP-ribosylation factor-binding protein GGA1; ADP-ribosylation factor-binding protein GGA2; ADP-ribosylation factor-binding protein GGA3

Gamma-tubulin is a universal component of microtubule organizing centers, which is essential for nuclear division and microtubule assembly in Aspergillus nidulans. TUBG1 mapped to within 20 kb of TUBG2 at 17q21. Gamma-tubulin is a minor protein, present at less than 1% the level of alpha- and beta-tubulin, and is limited to the centrosome. Gamma-tubulin is present in Drosophila melanogaster and Homo sapiens.
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: One other very common name is tubg1 antibody