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SKU:MA1042
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:GAP-7B10
Application:IHC-P, IHC-F, WB
Price: $200.00
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Overview

Product Name Anti-GAP43 Antibody (Monoclonal, GAP-7B10)
SKU/Catalog Number MA1042
Description Mouse IgG monoclonal antibody for GAP43, growth associated protein 43 (GAP43) detection. Tested with WB, IHC-P, IHC-F in Human; mouse; rat. No cross reactivity with otherproteins.
Cite This Product Anti-GAP43 Antibody (Monoclonal, GAP-7B10) GAP-7B10 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1042)
Replacement Item This antibody may replace the following items: sc-10786|sc-135697|sc-135915|sc-17109|sc-17109-R|sc-17790|sc-33705|sc-7457|sc-7458 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG2a
Validated Species Human, Mouse, Rat
Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen GAP-43 from neonatal rat forebrain membranes.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number GAP-7B10
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG2a

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Gap43
Protein Name Neuromodulin
Molecular Weight 23603 MW
Protein Function This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction (By similarity). .
Sequence Similarities Belongs to the neuromodulin family.
Subcellular Localization Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Cell projection, filopodium membrane ; Peripheral membrane protein . Cytoplasmic surface of growth cone and synaptic plasma membranes.
Uniprot ID P07936
Alternative Names Neuromodulin;Axonal membrane protein GAP-43;Growth-associated protein 43;Protein F1;Gap43;
Research Areas |neuroscience|cell type marker|neuron marker|growth cone| neuroscience|synapse marker|neurology process|growth and development|axonal guidance proteins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Neuromodulin

GAP43 is expressed by developing and regenerating neurons, and to a lesser extent, reactive glial cells. It is used widely to specifically label injured neurons and to score neuronal regeneration. GAP43 is also a neuronal growth cone protein thought to be involved in pathfinding. GAP43 is considered to be a crucial component of an effective regenerative response in the nervous system.

Anti-GAP43 Antibody (Monoclonal, GAP-7B10) Images

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Anti-GAP43 Antibody (Monoclonal, GAP-7B10)
Anti- GAP43 antibody, MA1042, IHC(P)
IHC(P): Rat Cardiac Muscle Tissue
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Publications

Hui Liu, Peizhen Lv, Yongjia Zhu, Huayu Wu, Kun Zhang, Fuben Xu, Li Zheng & Jinmin Zhao Scientific Reports 7, Article number: 39869 (2017) doi:10.1038/srep39869 Salidroside promotes peripheral nerve regeneration based on tissue engineering strategy using Schwann cells and PLGA: in vitro and in vivo
Zou H, Ding Y, Shi W, Xu X, Gong A, Zhang Z, Liu J. Cell Mol Neurobiol. 2015 Apr;35(3):313-22. Doi: 10.1007/S10571-014-0126-X. Epub 2014 Oct 29. Microrna-29C/Pten Pathway Is Involved In Mice Brain Development And Modulates Neurite Outgrowth In Pc1...
Zou H, Ding Y, Wang K, Xiong E, Peng W, Du F, Zhang Z, Liu J, Gong A. Neuroscience. 2015 Apr 16;291:289-300. Doi: 10.1016/J.Neuroscience.2015.01.055. Epub 2015 Feb 7. Microrna-29A/Pten Pathway Modulates Neurite Outgrowth In Pc12 Cells.
Nie X, Deng M, Yang M, Liu L, Zhang Y, Wen X. Cell Biochem Biophys. 2014 Jan;68(1):163-72. Doi: 10.1007/S12013-013-9683-8. Axonal Regeneration And Remyelination Evaluation Of Chitosan/Gelatin-Based Nerve Guide Combined With Transforming Growth Fac...
Chen Y, Wei G, Nie H, Lin Y, Tian H, Liu Y, Yu X, Cheng S, Yan R, Wang Q, Liu Dh, Deng W, Lai Y, Zhou Jh, Zhang Sx, Lin Ww, Chen Df. Brain Res. 2014 Mar 13;1552:41-54. Doi: 10.1016/J.Brainres.2014.01.005. Epub 2014 Jan 20. ??-Asarone Prevents Autop...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to gap 43 antibody, gap43 antibody, growth associated protein 43 antibody, growth-associated protein 43 antibody