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SKU:PA1037
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Polyclonal
Application:IHC-P, WB
Price: $200.00
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Overview

Product Name Anti-GAP43 Antibody
SKU/Catalog Number PA1037
Description Rabbit IgG polyclonal antibody for Neuromodulin(GAP43) detection. Tested with WB, IHC-P in Human;Mouse;Rat.
Cite This Product Anti-GAP43 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1037)
Replacement Item This antibody may replace the following items: sc-10786|sc-135697|sc-135915|sc-17109|sc-17109-R|sc-17790|sc-33705|sc-7457|sc-7458 from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human GAP43(216-238aa KPKESARQDEGKEEEPEADQEHA), different from the related mouse and rat sequences by one amino acid.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name GAP43
Protein Name Neuromodulin
Molecular Weight 24803 MW
Protein Function This protein is associated with nerve growth. It is a major component of the motile "growth cones" that form the tips of elongating axons. Plays a role in axonal and dendritic filopodia induction. .
Sequence Similarities Belongs to the neuromodulin family.
Subcellular Localization Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell projection, growth cone membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction, synapse . Cell projection, filopodium membrane ; Peripheral membrane protein . Cytoplasmic surface of growth cone and synaptic plasma membranes.
Uniprot ID P17677
Alternative Names Neuromodulin;Axonal membrane protein GAP-43;Growth-associated protein 43;Neural phosphoprotein B-50;pp46;GAP43;
Research Areas |neuroscience|cell type marker|neuron marker|growth cone| neuroscience|synapse marker|neurology process|growth and development|axonal guidance proteins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Neuromodulin

Growth Associated Protein-43(GAP-43), also known as nerve growth-related growth peptide GAP43, shares a high degree of homology between the sequence of the human gene and the rat gene. GAP-43 is considered a crucial component of an effective regenerative response in the nervous system. Somatic cell hybrids demonstrate localization of the GAP-43 gene to human chromosome 3 and to mouse chromosome 16. GAP-43 has been termed a "growth" or "plasticity" protein because it is expressed at high levels in neuronal growth cones during development and during axonal regeneration. GAP-43 regulates growth of axons and modulates the formation of new connections.

Anti-GAP43 Antibody Images

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Anti-GAP43 Antibody
Anti-GAP43 antibody, PA1037, Western blotting
All lanes: Anti GAP43 (PA1037) at 0.5ug/ml
Lane 1: U87 Whole Cell Lysate at 40ug
Lane 2: Rat Brain Tissue Lysate at 50ug
Lane 3: Mouse Brain Tissue Lysate at 50ug
Predicted bind size: 43KD
Observed bind size: 43KD
Anti-GAP43 Antibody
Anti-GAP43 antibody, PA1037, IHC(P)
IHC(P): Rat Brain Tissue
Anti-GAP43 Antibody
Anti-GAP43 antibody, PA1037, IHC(P)
IHC(P): Human Glioma Tissue
Anti-GAP43 Antibody
Anti-GAP43 antibody, PA1037, IHC(P)
IHC(P): Human Meningeoma Tissue
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Publications

Yuan Q, Sun L, Yu H, An C, Biosci Biotechnol Biochem. 2017 Apr 10:1-8. doi: 10.1080/09168451.2017.1313695. Human microvascular endothelial cell promotes the development of dorsal root ganglion neurons via BDNF pathway in a co-culture system.
Zou H, Ding Y, Shi W, Xu X, Gong A, Zhang Z, Liu J. Cell Mol Neurobiol. 2015 Apr;35(3):313-22. Doi: 10.1007/S10571-014-0126-X. Epub 2014 Oct 29. Microrna-29C/Pten Pathway Is Involved In Mice Brain Development And Modulates Neurite Outgrowth In Pc1...
Zou H, Ding Y, Wang K, Xiong E, Peng W, Du F, Zhang Z, Liu J, Gong A. Neuroscience. 2015 Apr 16;291:289-300. Doi: 10.1016/J.Neuroscience.2015.01.055. Epub 2015 Feb 7. Microrna-29A/Pten Pathway Modulates Neurite Outgrowth In Pc12 Cells.
Nie X, Deng M, Yang M, Liu L, Zhang Y, Wen X. Cell Biochem Biophys. 2014 Jan;68(1):163-72. Doi: 10.1007/S12013-013-9683-8. Axonal Regeneration And Remyelination Evaluation Of Chitosan/Gelatin-Based Nerve Guide Combined With Transforming Growth Fac...
Chen Y, Wei G, Nie H, Lin Y, Tian H, Liu Y, Yu X, Cheng S, Yan R, Wang Q, Liu Dh, Deng W, Lai Y, Zhou Jh, Zhang Sx, Lin Ww, Chen Df. Brain Res. 2014 Mar 13;1552:41-54. Doi: 10.1016/J.Brainres.2014.01.005. Epub 2014 Jan 20. ??-Asarone Prevents Autop...

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to gap 43 antibody, gap43 antibody, growth associated protein 43 antibody, growth-associated protein 43 antibody