Anti-GATA1 Rabbit Monoclonal Antibody

GATA-1 antibody

Boster Bio Anti-GATA1 Rabbit Monoclonal Antibody catalog # M00408. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human. Cited in 1 publication(s).

Product Info Summary

SKU: M00408
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IP, IF, IHC, ICC, WB

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Product Name

Anti-GATA1 Rabbit Monoclonal Antibody

View all GATA-1 Antibodies

SKU/Catalog Number

M00408

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-GATA1 Rabbit Monoclonal Antibody catalog # M00408. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GATA1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00408)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABCB-7

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human GATA1

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00408 is reactive to GATA1 in Human

Applications

M00408 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

52 kDa

Calculated molecular weight

42.751kDa

Background of GATA-1

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For GATA1 (Source: Uniprot.org, NCBI)

Gene Name

GATA1

Full Name

Erythroid transcription factor

Weight

42.751kDa

Alternative Names

Eryf1; ERYF1GATA-binding protein 1 (globin transcription factor 1); GATA binding protein 1 (globin transcription factor 1); GATA1; GATA-1; GATA-1erythroid transcription factor; GATA-binding factor 1; GF-1; GF1globin transcription factor 1; NF-E1 DNA-binding protein; NFE1erythroid transcription factor 1; transcription factor GATA1; XLTT GATA1 ERYF1, GATA-1, GF-1, GF1, NF-E1, NFE1, XLANP, XLTDA, XLTT GATA binding protein 1 erythroid transcription factor|GATA-binding factor 1|NF-E1 DNA-binding protein|erythroid transcription factor 1|globin transcription factor 1|nuclear factor, erythroid 1|transcription factor GATA1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GATA1, check out the GATA1 Infographic

GATA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GATA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00408 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zhang J,Zhuang R,Zhang X,Hu W,Cheng K,Jiang D,Shen S,Zhang Y,Ding Y,Zhang Y.CD226 is involved in megakaryocyte activation and early-stage differentiation.Mol Immunol.2019 Mar;107:123-131.doi:10.1016/j.molimm.2019.01.013.Epub 2019 Feb 6.PMID:30738249.
Species: Human,Mouse
M00408 usage in article: APP:WB, SAMPLE:DAMI CELL, DILUTION:NA

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3 Customer Q&As for Anti-GATA1 Rabbit Monoclonal Antibody

Question

See attached the WB image, lot number and protocol we used for erythrocyte using anti-GATA1 Rabbit Monoclonal antibody M00408. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-25

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-25

Question

I see that the anti-GATA1 Rabbit Monoclonal antibody M00408 works with IF, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-04-12

Answer

You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-04-12

Question

We are currently using anti-GATA1 Rabbit Monoclonal antibody M00408 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2017-11-16

Answer

The anti-GATA1 Rabbit Monoclonal antibody (M00408) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-11-16

Order DetailsPrice
M00408

100μL

$370
M00408-30ul

30μL sample

$99
M00408-carrier-free

Carrier Free

$370

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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