Anti-GATA3 Rabbit Monoclonal Antibody

GATA-3 antibody

Boster Bio Anti-GATA3 Rabbit Monoclonal Antibody catalog # M00593. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human. Cited in 1 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: M00593
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

Product Name

Anti-GATA3 Rabbit Monoclonal Antibody

View all GATA-3 Antibodies

SKU/Catalog Number

M00593

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-GATA3 Rabbit Monoclonal Antibody catalog # M00593. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GATA3 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00593)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

ABBH-7

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human GATA3

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M00593 is reactive to GATA3 in Human

Applications

M00593 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

32-42 kDa, 65 kDa, 75 kDa

Calculated molecular weight

47.916kDa

Background of GATA-3

Dynamin-related GTPase required for mitochondrial fusion and regulation of apoptosis. May form a diffusion barrier for proteins stored in mitochondrial cristae. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:100

Validation Images & Assay Conditions

Gene/Protein Information For GATA3 (Source: Uniprot.org, NCBI)

Gene Name

GATA3

Full Name

Trans-acting T-cell-specific transcription factor GATA-3

Weight

47.916kDa

Alternative Names

131320; GATA binding protein 3; GATA3; GATA-3; GATA-binding factor 3; GATA-binding protein 3; HDRMGC5199; MGC2346; MGC5445; trans-acting T-cell-specific transcription factor GATA-3 GATA3 HDR, HDRS GATA binding protein 3 trans-acting T-cell-specific transcription factor GATA-3|GATA-binding factor 3

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GATA3, check out the GATA3 Infographic

GATA3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GATA3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

M00593 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Ahluwalia A,Hoa N,Ge L,Blumberg B,Levin ER.Mechanisms by Which Membrane and Nuclear ER Alpha Inhibit Adipogenesis in Cells Isolated From Female Mice. Endocrinology. 2020 Nov 1;161(11):bqaa175.doi: 10.1210/endocr/bqaa175.PMID:32976570.
Species: Mouse
M00593 usage in article: APP:CHROMATIN IMMUNOPRECIPITATION, SAMPLE:3T3-L1 CELLS, DILUTION:NA

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1 Reviews For Anti-GATA3 Rabbit Monoclonal Antibody

0

Good GATA3 Antibody from BosterBio--Xiangrong Geng Internal medicine University of Michigan Postdoctoral Researcher

Excellent

Source: Biocompare.com

Application Western Blot
Sample HEK293T
Detection ECL

"I used this antibody for western blotting and immunoprecipitation. It works well in both of them. Very good antibody."

Verified customer

Submitted

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16 Customer Q&As for Anti-GATA3 Rabbit Monoclonal Antibody

Question

Our lab were satisfied with the WB result of your anti-GATA3 Rabbit Monoclonal antibody. However we have seen positive staining in t-cell nucleus. using this antibody. Is that expected? Could you tell me where is GATA3 supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-04-29

Answer

From what I have seen in literature, t-cell does express GATA3. Generally GATA3 expresses in nucleus. Regarding which tissues have GATA3 expression, here are a few articles citing expression in various tissues:
Cervix, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
T-cell, Pubmed ID: 1827068, 2017177, 2050118

Boster Scientific Support

Answered: 2020-04-29

Question

I would like using your anti-GATA3 Rabbit Monoclonal antibody for immune system development studies. Has this antibody been tested with western blotting on jurkat cell lysate? We would like to see some validation images before ordering.

E. Rodriguez

Verified customer

Asked: 2020-04-08

Answer

We appreciate your inquiry. This M00593 anti-GATA3 Rabbit Monoclonal antibody is validated on jurkat cell lysate. It is guaranteed to work for ChIP, Flow Cytometry, IF, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-04-08

Question

I was wanting to use your anti-GATA3 Rabbit Monoclonal antibody for IHC for human leukemic t-cell on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukemic t-cell identification?

Verified Customer

Verified customer

Asked: 2020-03-16

Answer

It shows on the product datasheet, M00593 anti-GATA3 Rabbit Monoclonal antibody has been tested for ChIP, Flow Cytometry, IF, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukemic t-cell in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2020-03-16

Question

I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-GATA3 Rabbit Monoclonal antibody M00593. Let me know if you need anything else.

N. Anderson

Verified customer

Asked: 2020-02-03

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-03

Question

Is there a BSA free version of anti-GATA3 Rabbit Monoclonal antibody M00593 available?

Verified Customer

Verified customer

Asked: 2020-01-29

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GATA3 Rabbit Monoclonal antibody M00593 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-29

Question

Our lab want to know about to test anti-GATA3 Rabbit Monoclonal antibody M00593 on human leukemic t-cell for research purposes, then I may be interested in using anti-GATA3 Rabbit Monoclonal antibody M00593 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

The products we sell, including anti-GATA3 Rabbit Monoclonal antibody M00593, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2020-01-02

Question

Our lab used your anti-GATA3 Rabbit Monoclonal antibody for IF on leukemic t-cell in a previous project. I am using human, and We want to use the antibody for Flow Cytometry next. you antibody examining leukemic t-cell as well as cervix placenta in our next experiment. Could you please give me some suggestion on which antibody would work the best for Flow Cytometry?

Verified Customer

Verified customer

Asked: 2020-01-02

Answer

I took a look at the website and datasheets of our anti-GATA3 Rabbit Monoclonal antibody and it seems that M00593 has been tested on human in both IF and Flow Cytometry. Thus M00593 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-02

Question

My question regarding product M00593, anti-GATA3 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00593 anti-GATA3 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-10-21

Question

I see that the anti-GATA3 Rabbit Monoclonal antibody M00593 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-08-29

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-08-29

Question

Does anti-GATA3 Rabbit Monoclonal antibody M00593 work for IHC with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2019-08-09

Answer

According to the expression profile of leukemic t-cell, GATA3 is highly expressed in leukemic t-cell. So, it is likely that anti-GATA3 Rabbit Monoclonal antibody M00593 will work for IHC with leukemic t-cell.

Boster Scientific Support

Answered: 2019-08-09

Question

Is this M00593 anti-GATA3 Rabbit Monoclonal antibody reactive to the isotypes of GATA3?

G. Zhao

Verified customer

Asked: 2019-08-09

Answer

The immunogen of M00593 anti-GATA3 Rabbit Monoclonal antibody is A synthesized peptide derived from human GATA3. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-08-09

Question

Please see the WB image, lot number and protocol we used for leukemic t-cell using anti-GATA3 Rabbit Monoclonal antibody M00593. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-06-12

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-12

Question

We have been able to see staining in human t-cell. What should we do? Is anti-GATA3 Rabbit Monoclonal antibody supposed to stain t-cell positively?

Verified Customer

Verified customer

Asked: 2019-04-18

Answer

According to literature t-cell does express GATA3. According to Uniprot.org, GATA3 is expressed in endometrium epithelium, t-cell, cervix placenta, leukemic t-cell, among other tissues. Regarding which tissues have GATA3 expression, here are a few articles citing expression in various tissues:
Cervix, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
T-cell, Pubmed ID: 1827068, 2017177, 2050118

Boster Scientific Support

Answered: 2019-04-18

Question

Is a blocking peptide available for product anti-GATA3 Rabbit Monoclonal antibody (M00593)?

Verified Customer

Verified customer

Asked: 2019-01-25

Answer

We do provide the blocking peptide for product anti-GATA3 Rabbit Monoclonal antibody (M00593). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-01-25

Question

We are currently using anti-GATA3 Rabbit Monoclonal antibody M00593 for human tissue, and we are content with the ICC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?

Verified Customer

Verified customer

Asked: 2017-09-08

Answer

The anti-GATA3 Rabbit Monoclonal antibody (M00593) has not been validated for cross reactivity specifically with goat tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-09-08

Question

Would M00593 anti-GATA3 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

T. Walker

Verified customer

Asked: 2013-08-12

Answer

You can see on the product datasheet, M00593 anti-GATA3 Rabbit Monoclonal antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-08-12

Order DetailsPrice
M00593

100μL

$370
M00593-30ul

30μL sample

$99
M00593-carrier-free

Carrier Free

$370

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