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Product Info Summary
|Application:||Flow Cytometry, IF, IHC, ICC, WB|
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Anti-GC / VDBP Rabbit Monoclonal Antibody
Boster Bio Anti-GC / VDBP Rabbit Monoclonal Antibody catalog # M03364. Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GC / VDBP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M03364)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human GC / VDBP
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M03364 is reactive to GC in Human
M03364 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Flow Cytometry 1:30
Validation Images & Assay Conditions
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Western blot analysis of GC/VDBP expression in Human plasma lysate (M03364).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GC monoclonal antibody (Catalog # M03364) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GC
Gene/Protein Information For GC (Source: Uniprot.org, NCBI)
Vitamin D-binding protein
DBP; DBP/GC; DBP-MAF; DBPVDBG; EC 18.104.22.168; Gc; gc-globulin; Gc-MAF; GRD3; group-specific component (vitamin D binding protein); Group-specific component; hDBP; VDB; VDBG; VDBP; Vitamin D BP; vitamin D-binding alpha-globulin; vitamin D-binding protein GC DBP, DBP-maf, DBP/GC, GRD3, Gc-MAF, GcMAF, HEL-S-51, VDB, VDBG, VDBP GC vitamin D binding protein vitamin D-binding protein|epididymis secretory protein Li 51|gc protein-derived macrophage activating factor|gc-globulin|group-specific component (vitamin D binding protein)|vitamin D-binding alpha-globulin|vitamin D-binding protein-macrophage activating factor*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on GC, check out the GC Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GC: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
No publications found for M03364
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