Anti-GCET2 Monoclonal Antibody

GCET2 antibody

Boster Bio Anti-GCET2 Monoclonal Antibody catalog # M12995. Tested in IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Product Info Summary

SKU: M12995
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC

Product Name

Anti-GCET2 Monoclonal Antibody

View all GCET2 Antibodies

SKU/Catalog Number

M12995

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-GCET2 Monoclonal Antibody catalog # M12995. Tested in IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GCET2 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M12995)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

AFHF-7

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human GCET2 Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M12995 is reactive to Gcsam in Human

Applications

M12995 is guaranteed for Flow Cytometry, IF, IHC, ICC Boster Guarantee

Observed Molecular Weight

58 kDa

Calculated molecular weight

20.988kDa

Background of GCET2

Putative transcription factor involved in pancreas development and function.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

IHC 1:100-1:500
ICC/IF 1:100-1:500
FC 1:100

Validation Images & Assay Conditions

Gene/Protein Information For Gcsam (Source: Uniprot.org, NCBI)

Gene Name

Gcsam

Full Name

Germinal center-associated signaling and motility protein

Weight

20.988kDa

Alternative Names

GAL; GCAT2; germinal center B-cell-expressed transcript 2 protein; germinal center expressed transcript 2; Germinal center-associated lymphoma protein; HGAL; MGC40441

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Gcsam, check out the Gcsam Infographic

Gcsam infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Gcsam: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-GCET2 Monoclonal Antibody

Question

We are currently using anti-GCET2 Monoclonal antibody M12995 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on feline tissues as well?

Verified Customer

Verified customer

Asked: 2019-06-03

Answer

The anti-GCET2 Monoclonal antibody (M12995) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-06-03

Question

Does anti-GCET2 Monoclonal antibody M12995 work for IHC with lymphoma?

Verified Customer

Verified customer

Asked: 2017-10-09

Answer

According to the expression profile of lymphoma, GCSAM is highly expressed in lymphoma. So, it is likely that anti-GCET2 Monoclonal antibody M12995 will work for IHC with lymphoma.

Boster Scientific Support

Answered: 2017-10-09

Question

I am interested in to test anti-GCET2 Monoclonal antibody M12995 on human lymphoma for research purposes, then I may be interested in using anti-GCET2 Monoclonal antibody M12995 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

M. Collins

Verified customer

Asked: 2017-01-12

Answer

The products we sell, including anti-GCET2 Monoclonal antibody M12995, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-01-12

Question

Is this M12995 anti-GCET2 Monoclonal antibody reactive to the isotypes of GCSAM?

P. Edwards

Verified customer

Asked: 2014-12-30

Answer

The immunogen of M12995 anti-GCET2 Monoclonal antibody is A synthesized peptide derived from human GCET2 Involved in the negative regulation of lymphocyte motility. It mediates the migration-inhibitory effects of IL6. Serves as a positive regulator of the RhoA signaling pathway. Enhancement of RhoA activation results in inhibition of lymphocyte and lymphoma cell motility by activation of its downstream effector ROCK. Is a regulator of B-cell receptor signaling, that acts through SYK kinase activation. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2014-12-30

Question

Will M12995 anti-GCET2 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

R. Patel

Verified customer

Asked: 2013-06-19

Answer

As indicated on the product datasheet, M12995 anti-GCET2 Monoclonal antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2013-06-19

Order DetailsPrice
M12995

100μL

$370
M12995-30ul

30μL sample

$99
M12995-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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M12995
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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