Anti-GCF GCFC2 Antibody

GCFC2 antibody

Boster Bio Anti-GCF GCFC2 Antibody (Catalog # A08456). Tested in WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A08456
Size: 100 µl
Reactive Species: Human, Mouse
Host: Rabbit
Application: WB

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Product Name

Anti-GCF GCFC2 Antibody

View all GCFC2 Antibodies

SKU/Catalog Number

A08456

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-GCF GCFC2 Antibody (Catalog # A08456). Tested in WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GCF GCFC2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08456)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from internal of human GCF.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A08456 is reactive to GCFC2 in Human, Mouse

Applications

A08456 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

89.385kDa

Background of GCFC2

Factor that represses transcription. It binds to the GC-rich sequences (5'-GCGGGGC-3') present in the epidermal growth factor receptor, beta-actin, and calcium-dependent protease promoters. Involved in pre-mRNA splicing through regulating spliceosome C complex formation. May play a role during late-stage splicing events and turnover of excised inrons.

Kageyama R., Cell 59:815-825(1989).
Hillier L.W., Nature 434:724-731(2005).
The MGC Project Team; Genome Res. 14:2121-2127(2004).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For GCFC2 (Source: Uniprot.org, NCBI)

Gene Name

GCFC2

Full Name

GC-rich sequence DNA-binding factor 2

Weight

89.385kDa

Superfamily

GCF family

Alternative Names

C2orf3; chromosome 2 open reading frame 3; DNABF; GC bindng factor; GCF; GCFGC binding factor; GC-rich sequence DNA-binding factor 2; GC-rich sequence DNA-binding factor; TCF9; TCF-9; transcription factor 9 (binds GC-rich sequences); Transcription factor 9 GCFC2 C2orf3, DNABF, GCF, TCF9 GC-rich sequence DNA-binding factor 2 intron Large complex component GCFC2|GC binding factor|GC bindng factor|TCF-9|transcription factor 9 (binds GC-rich sequences)

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GCFC2, check out the GCFC2 Infographic

GCFC2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GCFC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-GCF GCFC2 Antibody

Question

Is this A08456 anti-GCF antibody reactive to the isotypes of GCFC2?

Verified Customer

Verified customer

Asked: 2019-12-03

Answer

The immunogen of A08456 anti-GCF antibody is Synthesized peptide derived from internal of human GCF. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-12-03

Question

Do you have a BSA free version of anti-GCF antibody A08456 available?

Verified Customer

Verified customer

Asked: 2019-05-28

Answer

I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GCF antibody A08456 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-05-28

Question

We are currently using anti-GCF antibody A08456 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it possible that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2018-11-12

Answer

The anti-GCF antibody (A08456) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2018-11-12

Question

Is a blocking peptide available for product anti-GCF antibody (A08456)?

Verified Customer

Verified customer

Asked: 2018-05-29

Answer

We do provide the blocking peptide for product anti-GCF antibody (A08456). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-05-29

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukemic t-cell using anti-GCF antibody A08456. Let me know if you need anything else.

E. Williams

Verified customer

Asked: 2017-10-16

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2017-10-16

Question

Will anti-GCF antibody A08456 work for WB with leukemic t-cell?

Verified Customer

Verified customer

Asked: 2017-08-11

Answer

According to the expression profile of leukemic t-cell, GCFC2 is highly expressed in leukemic t-cell. So, it is likely that anti-GCF antibody A08456 will work for WB with leukemic t-cell.

Boster Scientific Support

Answered: 2017-08-11

Question

I see that the anti-GCF antibody A08456 works with WB, what is the protocol used to produce the result images on the product page?

D. Jackson

Verified customer

Asked: 2015-10-01

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-10-01

Order DetailsPrice
A08456-100uL

100uL

$360

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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