Product Info Summary
SKU: | A02948-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-GCLM Antibody
SKU/Catalog Number
A02948-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-GCLM Antibody catalog # A02948-1. Tested in WB,ICC/IF,IHC,Flow Cytometry,IP applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GCLM Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02948-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human CRF-RII
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02948-1 is reactive to GCLM in Human, Mouse, Rat
Applications
A02948-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
30.727kDa
Background of GCLM
May play an important role in fibrillogenesis by controlling lateral growth of collagen II fibrils.
Yoshioka H., J. Biol. Chem. 265:6423-6426(1990).
Annunen S., Am. J. Hum. Genet. 65:974-983(1999).
Zhidkova N.I., J. Biol. Chem. 270:9486-9493(1995).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:1,000
ICC: 1:50-1:200
IHC: 1:50-1:100
FC: 1:50-1:100
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunocytochemistry staining of GCLM using Anti-GCLM Antibody (A02948-1).
ICC staining GCLM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehydeClick image to see more details
Figure 1. Western blotting validation for Anti-GCLM Antibody A02948-1
Western blot analysis of GCLM on different cell lysates using anti-GCLM antibody at 1/500 dilution. Positive control: Lane 1: A431 Lane 2: PC-12 Lane 3: NIH-3T3Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
Protein Target Info & Infographic
Gene/Protein Information For GCLM (Source: Uniprot.org, NCBI)
Gene Name
GCLM
Full Name
Glutamate--cysteine ligase regulatory subunit
Weight
30.727kDa
Superfamily
aldo/keto reductase family
Alternative Names
Gamma-ECS regulatory subunit; Gamma-glutamylcysteine synthetase regulatory subunit; GCS light chain; GLCLRglutamate--cysteine ligase regulatory subunit; glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory(30.8kD); Glutamate--cysteine ligase modifier subunit; glutamate-cysteine ligase regulatory protein; glutamate-cysteine ligase, modifier subunit; GSC light chain GCLM GLCLR glutamate-cysteine ligase modifier subunit glutamate--cysteine ligase regulatory subunit|GCS light chain|GSC light chain|gamma-ECS regulatory subunit|gamma-glutamylcysteine synthetase regulatory subunit|glutamate-cysteine ligase (gamma-glutamylcysteine synthetase), regulatory (30.8kD)|glutamate-cysteine ligase modifier subunit delta2 alternative splicing|glutamate-cysteine ligase regulatory protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GCLM, check out the GCLM Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GCLM: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GCLM Antibody (A02948-1)
Hello CJ!
No publications found for A02948-1
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