Anti-GCLM Monoclonal Antibody

Rabbit IgG Monoclonal antibody for P48507 detection. Tested positive for WB IHC IF/IF IP FC in Human Mouse Rat

SKU M02948
Size 100ug
Reactivity Human, Mouse, Rat
Clonality Monoclonal
Clone Number AEOH-7
Host Rabbit
Ig Isotype N/A
Applications IP, IF, WB

Overview

Product Name Anti-GCLM Monoclonal Antibody
See all GCLM primary antibodies, ELISA kits and proteins
SKU/Catalog Number M02948
Storage & Handling For proper storage of Anti-GCLM Monoclonal Antibody: Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at +4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Size 100ug
Description Rabbit IgG Monoclonal antibody for P48507 detection. Tested positive for WB IHC IF/IF IP FC in Human Mouse Rat
Cite This Product Anti-GCLM Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02948)
Specificity Anti-GCLM Monoclonal Antibody (M02948) reacts with Human, Mouse, Rat GCLM, in native form and recombinant. Superfamily members of GCLM are not reactive to M02948.
Host Rabbit
Form Liquid
Reconstitution Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Immunogen A synthesized peptide derived from human GCLM Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
Reactivity Human, Mouse, Rat

Assay Details

Our Boster Quality Guarantee for Anti-GCLM Monoclonal Antibody covers its use in the following applications.

*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.

Assay Dilutions Overview

Concentration: 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
WB 1:500~1:2000 IHC 1:50~1:200 ICC/IF 1:50~1:200 IP 1:50 FC 1:100

Boster's Compatible Products

The following reagents are used to generate the images below for Anti-GCLM Monoclonal Antibody (M02948).

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

/M/0/M02948-GCLM-WB-test-1.jpg

Figure 1. Western blot analysis of GCLM using anti-GCLM antibody (M02948).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GCLM antigen affinity purified polyclonal antibody (Catalog # M02948) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for GCLM.

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P48507
Gene Name GCLM
Protein Name Glutamate--cysteine ligase regulatory subunit
Tissue Specificity In all tissues examined. Highest levels in skeletal muscle.
Alternative Names Glutamate--cysteine ligase regulatory subunit;GCS light chain;Gamma-ECS regulatory subunit;Gamma-glutamylcysteine synthetase regulatory subunit;Glutamate--cysteine ligase modifier subunit;GCLM;GLCLR;
Subcellular Localization Nucleus .
Molecular Weight 84026 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Binds specifically to the DNA sequence 5'-AACAAT-3'. Activates transcription of COL2A1 and AGC1 in vitro.
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background Transcription factor SOX-5 is a protein that in humans is encoded by the SOX5 gene. It is located on 12p12.1. This gene encodes a member of the SOX (SRY-related HMG-box) family of transcription factors involved in the regulation of embryonic development and in the determination of the cell fate. The encoded protein may act as a transcriptional regulator after forming a protein complex with other proteins. In addition, the encoded protein may play a role in chondrogenesis. A pseudogene of this gene is located on chromosome 8. Multiple transcript variants encoding distinct isoforms have been identified for this gene.

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In stock
Order Product
M02948
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$299.00

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