|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-Gelsolin Antibody (Monoclonal, GS-2C4)|
|Description||Mouse IgG monoclonal antibody for Gelsolin, gelsolin (GSN) detection. Tested with WB, IHC-P, IHC-F in Human; rabbit. No cross reactivity with other proteins.|
|Cite This Product||Anti-Gelsolin Antibody (Monoclonal, GS-2C4) GS-2C4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1044)|
|Replacement Item||This antibody may replace the following items: sc-22600|sc-22602|sc-271001|sc-398244|sc-48769|sc-514502|sc-57509|sc-6405|sc-6406 from Santa Cruz Biotechnology.|
|Validated Species||Human, Rabbit|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||Human plasma gelsolin.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
|Concentration||Add 1ml of PBS buffer will yield a concentration of 100ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Molecular Weight||86068 MW|
|Protein Function||Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis (By similarity). .|
|Tissue Specificity||GSN: Phagocytic cells, platelets, fibroblasts,nonmuscle cells, smooth and skeletal muscle cells.|
|Sequence Similarities||Belongs to the villin/gelsolin family.|
|Subcellular Localization||Isoform 1: Secreted .|
|Alternative Names||Gelsolin;Actin-depolymerizing factor;ADF;Brevin;Gsn;|
|Research Areas|||signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin binding proteins||
Background for Gelsolin
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Gelsolin Antibody (Monoclonal, GS-2C4) by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 1-2μg/ml, Human, rabbit, By Heat
Immunohistochemistry(Frozen Section), 1-2μg/ml, Human, rabbit, -
Western blot, 0.5-1μg/ml, Human, rabbit
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Gelsolin Antibody (Monoclonal, GS-2C4) Images
Click the images to enlarge.
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Intestinal Cancer Tissue