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SKU:MA1044
Pack Size:100μg/vial
Sample Size:30ug for $99, contact us for details
Clonality:Monoclonal
Clone Number:GS-2C4
Application:IHC, WB
Price: $200.00
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Overview

Product Name Anti-Gelsolin Antibody (Monoclonal, GS-2C4)
SKU/Catalog Number MA1044
Description Mouse IgG monoclonal antibody for Gelsolin, gelsolin (GSN) detection. Tested with WB, IHC-P, IHC-F in Human; rabbit. No cross reactivity with other proteins.
Cite This Product Anti-Gelsolin Antibody (Monoclonal, GS-2C4) GS-2C4 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1044)
Replacement Item This antibody may replace the following items: sc-22600|sc-22602|sc-271001|sc-398244|sc-48769|sc-514502|sc-57509|sc-6405|sc-6406 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Rabbit
Application IHC, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen Human plasma gelsolin.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number GS-2C4
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Gsn
Protein Name Gelsolin
Molecular Weight 86068 MW
Protein Function Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis (By similarity). .
Tissue Specificity GSN: Phagocytic cells, platelets, fibroblasts,nonmuscle cells, smooth and skeletal muscle cells.
Sequence Similarities Belongs to the villin/gelsolin family.
Subcellular Localization Isoform 1: Secreted .
Uniprot ID Q68FP1
Alternative Names Gelsolin;Actin-depolymerizing factor;ADF;Brevin;Gsn;
Research Areas |signal transduction|cytoskeleton / ecm|cytoskeleton|microfilaments|actin etc|actin binding proteins|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Gelsolin

Gelsolin, a protein of leukocytes, platelets, and other cells, severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A gelsolin variant with 23 more N-terminal amino acids is a plasma component probably involved in the clearance of actin, the most abundant human protein, from the circulation. Gelsolin is located in 9q34. Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain.

Anti-Gelsolin Antibody (Monoclonal, GS-2C4) Images

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Anti-Gelsolin Antibody (Monoclonal, GS-2C4)
Anti- Gelsolin antibody, MA1044, IHC(P)
IHC(P): Human Lung Cancer Tissue
Anti-Gelsolin Antibody (Monoclonal, GS-2C4)
Anti- Gelsolin antibody, MA1044, IHC(P)
IHC(P): Human Intestinal Cancer Tissue
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FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.