Show Order Info
SKU:MA1045
Clonality:Monoclonal
Clone Number:G-A-5
Application:IHC-P, IHC-F, WB
Price: $200.00
Quantity:
Technical
Specs
Kit
Components
Gene
Information
Application
Data & Images
Reviews
Publications
FAQs

Overview

Product Name Anti-GFAP Antibody (Monoclonal, G-A-5)
Description Mouse IgG monoclonal antibody for GFAP, glial fibrillary acidic protein (GFAP) detection. Tested with WB, IHC-P, IHC-F in Human;pig;rat. No cross reactivity with other proteins.
Cite This Product Anti-GFAP Antibody (Monoclonal, G-A-5) G-A-5 (Boster Biological Technology, Pleasanton CA, USA, Catalog # MA1045)
Replacement Item This antibody may replace the following items: sc-6172|sc-11422|sc-130701|sc-6418|sc-11422|sc-130701|sc-6172|sc-6418 from Santa Cruz Biotechnology.
Host Mouse
Isotype Mouse IgG1
Validated Species Human, Pig, Rat
Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Mouse IgG (EK1001) for Western blot, and HRP Conjugated anti-Mouse IgG Super Vision Assay Kit (SV0001-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2016!
Immunogen GFAP from pig spinal cord.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Monoclonal
Clone Number G-A-5
Form Lyophilized
Contents Mouse ascites fluid, 1.2% sodium acetate, 2mg BSA, with 0.01mg NaN3 as preservative.
*carrier free antibody available upon request.
Concentration Add 1ml of PBS buffer will yield a concentration of 100ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Ascites
Isotype Mouse IgG1

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name Gfap
Protein Name Glial fibrillary acidic protein
Gene Full Name Glial fibrillary acidic protein
Protein Function GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the centralnervous system, distinguishes astrocytes from other glial cells.
Tissue Specificity Expressed in the cortex and hippocampus.Expression decreases following acute and chronic corticosteronetreatment..
Sequence Similarities Belongs to the intermediate filament family.
Subcellular Localization Cytoplasm. Associatedwith intermediate filaments..
Uniprot ID Gfap: P47819
Alternative Names Glial fibrillary acidic protein; GFAP; Gfap;
Research Areas neuroscience|cell adhesion proteins|cytoskeletal proteins|intermediate filaments|cell type marker|glia marker|astrocyte marker|signal transduction|cytoskeleton / ecm|cytoskeleton|class ii|gfap|tags & cell markers|cell type markers|neuroscience markers|glial|stem cells|neural stem cells|glial restricted lineage|astrocyte|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Glial fibrillary acidic protein

Glial fibrillary acidic protein(GFAP) is an intermediate filament protein of 52Kda. GFAP gene is mapped to human 17q21. GFAP is a useful marker of astroglia in the brain. Mutations in GFAP, encoding glial fibrillary acidic protein, are associated with Alexander disease.

Anti-GFAP Antibody (Monoclonal, G-A-5) Images

Click the images to enlarge.

Anti-GFAP Antibody (Monoclonal, G-A-5)
Anti- GFAP antibody, MA1045, IHC(P)
IHC(P): Rat Brain Tissue
Write a review for MA1045

Publications

Zhou H, Cheng X, Rao L, Li T, Duan Yy. Acta Biomater. 2013 May;9(5):6439-49. Doi: 10.1016/J.Actbio.2013.01.042. Epub 2013 Feb 9. Poly(3,4-Ethylenedioxythiophene)/Multiwall Carbon Nanotube Composite Coatings For Improving The Stability Of Microelec...
Zhao Bq, Guo Yr, Li Xl, Zang T, Qu Hy, Zhou Jp, Li Q, Sun Mj. Int J Pharm. 2011 Feb 28;405(1-2):1-8. Doi: 10.1016/J.Ijpharm.2010.11.018. Epub 2010 Nov 18. Amelioration Of Dementia Induced By A?? 22-35 Through Rectal Delivery Of Undecapeptide-Hegf T...
Zhang S, Xie R, Wan F, Ye F, Guo D, Lei T. Oncol Lett. 2013 Dec;6(6):1649-1655. Epub 2013 Oct 11. Identification Of U251 Glioma Stem Cells And Their Heterogeneous Stem-Like Phenotypes.
Wang H, Li C, Wang H, Mei F, Liu Z, Shen Hy, Xiao L. Neurosci Bull. 2013 Apr;29(2):251-9. Doi: 10.1007/S12264-013-1323-1. Epub 2013 Apr 5. Cuprizone-Induced Demyelination In Mice: Age-Related Vulnerability And Exploratory Behavior Deficit.
Xu Dh, Yan M, Li Hd, Fang Pf, Liu Yw. Eur J Pharmacol. 2012 Sep 5;690(1-3):68-76. Doi: 10.1016/J.Ejphar.2012.06.032. Epub 2012 Jun 30. Influence Of P-Glycoprotein On Brucine Transport At The In Vitro Blood-Brain Barrier.
Yu Cz, Liu Yp, Liu S, Yan M, Hu Sj, Song Xj. Eur J Pain. 2014 Jan;18(1):76-85. Doi: 10.1002/J.1532-2149.2013.00390.X. Epub 2013 Sep 5. Systematic Administration Of B Vitamins Attenuates Neuropathic Hyperalgesia And Reduces Spinal Neuron Injury Fol...
Bai Y, Xu G, Xu M, Li Q, Qin X. Int J Mol Med. 2014 Dec;34(6):1473-82. Doi: 10.3892/Ijmm.2014.1946. Epub 2014 Sep 24. Inhibition Of Src Phosphorylation Reduces Damage To The Blood-Brain Barrier Following Transient Focal Cerebral Ischemia In Rats.
Zhang L, Wei Ws, Li Yj, Wang Y. Neuropathology. 2011 Apr;31(2):112-21. Doi: 10.1111/J.1440-1789.2010.01137.X. A Rat Model Of Mild Cognitive Impairment Associated With Vascular Factor.
Zhang X, Zheng Z, Shu B, Liu X, Zhang Z, Liu Y, Bai B, Hu Q, Mao P, Wang H. J Virol. 2013 Nov;87(22):12407-21. Doi: 10.1128/Jvi.02090-13. Epub 2013 Sep 11. Human Astrocytic Cells Support Persistent Coxsackievirus B3 Infection.
Huang F, Wang J, Chen A. J Huazhong Univ Sci Technolog Med Sci. 2006;26(2):206-10. Effects Of Co-Grafts Mesenchymal Stem Cells And Nerve Growth Factor Suspension In The Repair Of Spinal Cord Injury.
Ma H, Yu B, Kong L, Zhang Y, Shi Y. Neurochem Res. 2012 Jan;37(1):69-83. Doi: 10.1007/S11064-011-0584-1. Epub 2011 Sep 7. Neural Stem Cells Over-Expressing Brain-Derived Neurotrophic Factor (Bdnf) Stimulate Synaptic Protein Expression And Promote ...
Huo Sj, Li Yc, Xie J, Li Y, Raisman G, Zeng Yx, He Jr, Weng Ch, Yin Zq. Curr Eye Res. 2012 Aug;37(8):749-58. Doi: 10.3109/02713683.2012.697972. Epub 2012 Jun 12. Transplanted Olfactory Ensheathing Cells Reduce Retinal Degeneration In Royal College...
Chen Sw, Chen Yx, Zhang Xr, Qian H, Chen Wz, Xie Wf. Gene Ther. 2008 Nov;15(21):1424-35. Doi: 10.1038/Gt.2008.93. Epub 2008 May 29. Targeted Inhibition Of Platelet-Derived Growth Factor Receptor-Beta Subunit In Hepatic Stellate Cells Ameliorates H...
Hu Ww, Wang Z, Zhang Ss, Jiang L, Zhang J, Zhang X, Lei Qf, Park Hj, Fang Wj, Chen Z. Biomaterials. 2014 Aug;35(26):7386-97. Doi: 10.1016/J.Biomaterials.2014.05.026. Epub 2014 Jun 2. Morphology And Functions Of Astrocytes Cultured On Water-Repelle...
Chu H, Sun J, Xu H, Niu Z, Xu M. Neurol Res. 2012 Nov;34(9):871-88. Doi: 10.1179/1743132812Y.0000000085. Epub 2012 Aug 13. Effect Of Periaqueductal Gray Melanocortin 4 Receptor In Pain Facilitation And Glial Activation In Rat Model Of Chronic Cons...
Zhang Y, Sun Lg, Ye Lp, Wang B, Li Y. Toxicol Mech Methods. 2008 Jan;18(9):751-7. Doi: 10.1080/15376510802390908. Lead-Induced Stress Response In Endoplasmic Reticulum Of Astrocytes In Cns.
Huo Sj, Li Y, Raisman G, Yin Zq. Brain Res. 2011 Mar 25;1382:238-44. Doi: 10.1016/J.Brainres.2010.12.079. Epub 2011 Jan 6. Transplanted Olfactory Ensheathing Cells Reduce The Gliotic Injury Response Of M??ller Cells In A Rat Model Of Retinitis Pigm...
Zhao Yy, Yuan Y, Chen Y, Jiang L, Liao Rj, Wang L, Zhang Xn, Ohtsu H, Hu Ww, Chen Z. Cns Neurosci Ther. 2015 May;21(5):454-62. Doi: 10.1111/Cns.12379. Epub 2015 Jan 24. Histamine Promotes Locomotion Recovery After Spinal Cord Hemisection Via Inhib...
Yuan H, Chen R, Wu L, Chen Q, Hu A, Zhang T, Wang Z, Zhu X. Mol Neurobiol. 2015 Apr;51(2):512-22. Doi: 10.1007/S12035-014-8717-6. Epub 2014 Apr 29. The Regulatory Mechanism Of Neurogenesis By Igf-1 In Adult Mice.
Wang R, Zhang X, Zhang J, Fan Y, Shen Y, Hu W, Chen Z. Plos One. 2012;7(5):E37574. Doi: 10.1371/Journal.Pone.0037574. Epub 2012 May 22. Oxygen-Glucose Deprivation Induced Glial Scar-Like Change In Astrocytes.
Zhang J, Gao Y, Shen Y, Li K. Indian J Med Res. 2009 Feb;129(2):164-9. Asymmetrical Release Of Interleukin-6 By Cultured Cerebral Cortical Astrocytes Treated With Lipopolysaccharide.
Shi Xh, Han J, Zhang J, Shi Q, Chen Jm, Xia Sl, Xie Zq, Shen Xj, Shan B, Lei Yj, Shi S, Zhou W, Zhang By, Gao C, Liu Yh, Song J, Guo Yj, Wang Dx, Xu Bl, Dong Xp. Infect Genet Evol. 2010 Mar;10(2):292-7. Doi: 10.1016/J.Meegid.2010.01.007. Epub 2010...
Zhang G, Zhao Z, Gao L, Deng J, Wang B, Xu D, Liu B, Qu Y, Yu J, Li J, Gao G. Pharmacol Biochem Behav. 2011 Jul;99(1):42-51. Doi: 10.1016/J.Pbb.2011.03.019. Epub 2011 Apr 1. Gypenoside Attenuates White Matter Lesions Induced By Chronic Cerebral Hy...
Ma H, Yu B, Kong L, Zhang Y, Shi Y. Mol Med Rep. 2011 Sep-Oct;4(5):849-56. Doi: 10.3892/Mmr.2011.510. Epub 2011 Jun 16. Transplantation Of Neural Stem Cells Enhances Expression Of Synaptic Protein And Promotes Functional Recovery In A Rat Model Of...
Zhang Gl, Deng Jp, Wang Bh, Zhao Zw, Li J, Gao L, Liu Bl, Xong Jr, Guo Xd, Yan Zq, Gao Gd. Behav Pharmacol. 2011 Oct;22(7):633-44. Doi: 10.1097/Fbp.0B013E32834Afef9. Gypenosides Improve Cognitive Impairment Induced By Chronic Cerebral Hypoperfusio...
Huile G, Shuaiqi P, Zhi Y, Shijie C, Chen C, Xinguo J, Shun S, Zhiqing P, Yu H. Biomaterials. 2011 Nov;32(33):8669-75. Doi: 10.1016/J.Biomaterials.2011.07.069. Epub 2011 Aug 16. A Cascade Targeting Strategy For Brain Neuroglial Cells Employing Nan...
Wu L, Miao S, Zou Lb, Wu P, Hao H, Tang K, Zeng P, Xiong J, Li Hh, Wu Q, Cai L, Ye Dy. J Mol Neurosci. 2012 Sep;48(1):185-200. Doi: 10.1007/S12031-012-9807-4. Epub 2012 Jun 3. Lipoxin A4 Inhibits 5-Lipoxygenase Translocation And Leukotrienes Biosy...
Chen Y, Wu X, Yu S, Lin X, Wu J, Li L, Zhao J, Zhao Y. Plos One. 2012;7(6):E40165. Doi: 10.1371/Journal.Pone.0040165. Epub 2012 Jun 29. Neuroprotection Of Tanshinone Iia Against Cerebral Ischemia/Reperfusion Injury Through Inhibition Of Macrophage...
Liu Lx, Zhou Xy, Li Cs, Liu Lq, Huang Sy, Zhou Sn. Neurol Sci. 2013 Sep;34(9):1671-8. Doi: 10.1007/S10072-013-1319-7. Epub 2013 Feb 13. Selenoprotein S Expression In The Rat Brain Following Focal Cerebral Ischemia.
Chen Z, Pan X, Georgakilas Ag, Chen P, Hu H, Yang Y, Tian S, Xia L, Zhang J, Cai X, Ge J, Yu K, Zhuang J. Cancer Lett. 2013 Aug 19;336(2):281-9. Doi: 10.1016/J.Canlet.2013.03.015. Epub 2013 Mar 21. Tetramethylpyrazine (Tmp) Protects Cerebral Neuro...
Chen X, Chen L, Chen J, Hu W, Gao H, Xie B, Wang X, Yin Z, Li S, Wang X. Brain Res. 2013 Nov 13;1538:151-8. Doi: 10.1016/J.Brainres.2013.02.025. Epub 2013 Mar 5. Adam17 Promotes U87 Glioblastoma Stem Cell Migration And Invasion.
Yu Q, Guo Z, Liu X, Ouyang Q, He C, Burnstock G, Yuan H, Xiang Z. Purinergic Signal. 2013 Dec;9(4):663-75. Doi: 10.1007/S11302-013-9379-Y. Epub 2013 Jul 23. Block Of P2X7 Receptors Could Partly Reverse The Delayed Neuronal Death In Area Ca1 Of The...
Sun Yx, Cong Yl, Liu Y, Jin B, Si L, Wang Ab, Cai H, Che Gy, Tang B, Wang Cf, Li Zy, Zhang Xm. Eur J Pharmacol. 2014 Oct 5;740:552-9. Doi: 10.1016/J.Ejphar.2014.06.032. Epub 2014 Jun 27. Schisandrin A And B Affect Subventricular Zone Neurogenesis ...
Xu X, Yang X, Xiong Y, Gu J, He C, Hu Y, Xiao F, Chen G, Wang X. J Neurochem. 2015 Apr;133(1):134-43. Doi: 10.1111/Jnc.13052. Epub 2015 Feb 26. Increased Expression Of Receptor For Activated C Kinase 1 In Temporal Lobe Epilepsy.
Lan Lf, Zheng L, Yang X, Ji Xt, Fan Yh, Zeng Js. Cns Neurosci Ther. 2015 May;21(5):410-6. Doi: 10.1111/Cns.12374. Epub 2015 Jan 22. Peroxisome Proliferator-Activated Receptor-?? Agonist Pioglitazone Ameliorates White Matter Lesion And Cognitive Imp...
Zhang L, Li H, Zeng S, Chen L, Fang Z, Huang Q. Neurosci Lett. 2015 Mar 30;591:30-4. Doi: 10.1016/J.Neulet.2015.02.023. Epub 2015 Feb 11. Long-Term Tracing Of The Brdu Label-Retaining Cells In Adult Rat Brain.
Zhu X, Dong J, Shen K, Bai Y, Zhang Y, Lv X, Chao J, Yao H. Brain Res Bull. 2015 May;114:70-8. Doi: 10.1016/J.Brainresbull.2015.04.002. Epub 2015 Apr 17. Nmda Receptor Nr2B Subunits Contribute To Ptz-Kindling-Induced Hippocampal Astrocytosis And O...
Shi J, Ma Y, Zheng M, Ruan Z, Liu J, Tian S, Zhang D, He X, Li G. Toxicol Ind Health. 2012 Feb;28(1):10-20. Doi: 10.1177/0748233711401264. Epub 2011 Mar 28. Effect Of Sub-Acute Exposure To Acrylamide On Gabaergic Neurons And Astrocytes In Weaning ...
Li F, Miao Zn, Xu Yy, Zheng Sy, Qin Md, Gu Yz, Zhang Xg. Mol Med Rep. 2012 Sep;6(3):625-30. Doi: 10.3892/Mmr.2012.968. Epub 2012 Jun 26. Transplantation Of Human Amniotic Mesenchymal Stem Cells In The Treatment Of Focal Cerebral Ischemia.
Liu Q, Cheng G, Wang Z, Zhan S, Xiong B, Zhao X. In Vitro Cell Dev Biol Anim. 2015 Mar;51(3):319-27. Doi: 10.1007/S11626-015-9875-1. Epub 2015 Mar 14. Bone Marrow-Derived Mesenchymal Stem Cells Differentiate Into Nerve-Like Cells In Vitro After Tr...
Lu Y, Yuan X, Ou Y, Cai Y, Wang S, Sun Q, Zhang W. Neural Regen Res. 2012 Jun 5;7(16):1205-12. Doi: 10.3969/J.Issn.1673-5374.2012.16.001. Autophagy And Apoptosis During Adult Adipose-Derived Stromal Cells Differentiation Into Neuron-Like Cells In ...
Wang X, Tu Q, Zhao B, An Y, Wang Jc, Liu W, Yuan Ms, Ahmed Sm, Xu J, Liu R, Zhang Y, Wang J. Biomaterials. 2013 Jan;34(4):1155-69. Doi: 10.1016/J.Biomaterials.2012.10.063. Epub 2012 Nov 17. Effects Of Poly(L-Lysine)-Modified Fe3O4 Nanoparticles On...
Ping Yf, Yao Xh, Jiang Jy, Zhao Lt, Yu Sc, Jiang T, Lin Mc, Chen Jh, Wang B, Zhang R, Cui Yh, Qian C, Wang Jm, Bian Xw. J Pathol. 2011 Jul;224(3):344-54. Doi: 10.1002/Path.2908. Epub 2011 May 27. The Chemokine Cxcl12 And Its Receptor Cxcr4 Promote...
Wu Y, Ge C, Zeng W, Zhang C. Stem Cells Dev. 2010 Feb;19(2):195-202. Doi: 10.1089/Scd.2008.0383. Induced Multilineage Differentiation Of Chicken Embryonic Germ Cells Via Embryoid Body Formation.
Liu S, Zhang Y, Zhao Y, Cui H, Cao C, Guo J. Cell Biochem Biophys. 2015 Jan;71(1):401-6. Doi: 10.1007/S12013-014-0212-1. Effects Of Hypothermia On S100B And Glial Fibrillary Acidic Protein In Asphyxia Rats After Cardiopulmonary Resuscitation.
Ge Xt, Lei P, Wang Hc, Zhang Al, Han Zl, Chen X, Li Sh, Jiang Rc, Kang Cs, Zhang Jn. Sci Rep. 2014 Oct 24;4:6718. Doi: 10.1038/Srep06718. Mir-21 Improves The Neurological Outcome After Traumatic Brain Injury In Rats.
Meng Xt, Chen D, Dong Zy, Liu Jm. Cell Biol Int. 2007 Jul;31(7):691-8. Epub 2007 Jan 19. Enhanced Neural Differentiation Of Neural Stem Cells And Neurite Growth By Amniotic Epithelial Cell Co-Culture.
Longxi P, Buwu F, Yuan W, Sinan G. Plos One. 2011;6(10):E26500. Doi: 10.1371/Journal.Pone.0026500. Epub 2011 Oct 28. Expression Of P53 In The Effects Of Artesunate On Induction Of Apoptosis And Inhibition Of Proliferation In Rat Primary Hepatic St...
Niu Z, Ma J, Chu H, Zhao Y, Feng W, Cheng Y. Neurosci Lett. 2012 Nov 7;529(2):112-7. Doi: 10.1016/J.Neulet.2012.09.034. Epub 2012 Sep 27. Melanocortin 4 Receptor Antagonists Attenuates Morphine Antinociceptive Tolerance, Astroglial Activation And ...

FAQs

Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: Can you provide monoclonal antibodies carrier free?
A: We cannot.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to gfap antibody, astrocyte antibody, glial fibrillary acidic protein antibody