Data & Images
|Product Name||Anti-GFAP Antibody|
|Description||Rabbit IgG polyclonal antibody for Glial fibrillary acidic protein(GFAP) detection. Tested with WB, IHC-P in Human;Mouse;Rat.|
|Cite This Product||Anti-GFAP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1239)|
|Replacement Item||This antibody may replace the following items: sc-6172|sc-11422|sc-130701|sc-6418|sc-11422|sc-130701|sc-6172|sc-6418 from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2016!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human GFAP(417-432aa DGEVIKESKQEHKDVM), identical to the related rat sequence, and different from the related mouse sequence by two amino acids.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Glial fibrillary acidic protein(GFAP)|
|Gene Full Name||Glial fibrillary acidic protein|
|Protein Function||GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the centralnervous system, distinguishes astrocytes from other glial cells.|
|Tissue Specificity||Expressed in cells lacking fibronectin..|
|Sequence Similarities||Belongs to the intermediate filament family.|
|Subcellular Localization||Cytoplasm.Associated with intermediate filaments.|
|Uniprot ID||GFAP: P14136
|Alternative Names||Glial fibrillary acidic protein; GFAP; GFAP;|
|Research Areas||neuroscience|cell adhesion proteins|cytoskeletal proteins|intermediate filaments|cell type marker|glia marker|astrocyte marker|signal transduction|cytoskeleton / ecm|cytoskeleton|class ii|gfap|tags & cell markers|cell type markers|neuroscience markers|glial|stem cells|neural stem cells|glial restricted lineage|astrocyte||
Background for Glial fibrillary acidic protein(GFAP)
At Boster we strive to provide the best Anti-GFAP Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Application||Concentration*||Tested Species||Predicted Species||Antigen Retrieval|
|Immunohistochemistry(Paraffin-embedded Section)||0.5-1μg/ml||Rat||Mouse||By Heat|
|Western blot||0.1-0.5μg/ml||Human, Mouse, Rat||-|
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
Other applications have not been tested.
Optimal dilutions should be determined by end users.
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-GFAP Antibody Images
Click the images to enlarge.
All lanes: Anti GFAP(PA1239) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 50ug
Lane 2: Mouse Brain Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Predicted bind size: 49KD
Observed bind size: 49KD
IHC(P): Rat Brain Tissue
Lane 1: Rat Brain Tissue Lysate
Lane 2: Rat Brain Tissue Lysate
Lane 3: Mouse Brain Tissue Lysate
Lane 4: Mouse Brain Tissue Lysate
- Review by Maria Teresa Dell'Anno
"This Antibody Works Perfectly!"
--Maria Teresa Dell'Anno, NEUROSCIENCE, YALE UNIVERSITY, POSTDOC
Applications Immunohistochemistry Sample Spinal cord Detection Confocal microscope
"I used it for IHC on frozen sections at a dilution of 1:500. It did not need several trials to optimize the protocol. No bad things overall. I will purchase it again."
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,