Anti-GFAP Antibody Picoband™

GFAP antibody

Boster Bio Anti-GFAP Antibody Picoband™ catalog # PB9082. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Pig. Cited in 10 publication(s).

Product Info Summary

SKU: PB9082
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: IF, IHC, WB

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Product Name

Anti-GFAP Antibody Picoband™

View all GFAP Antibodies

SKU/Catalog Number

PB9082

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-GFAP Antibody Picoband™ catalog # PB9082. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Pig.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GFAP Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9082)

Host

Rabbit

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human GFAP recombinant protein (Position: Q93-M432). Human GFAP shares 94% amino acid (aa) sequence identity with both mouse and rat GFAP.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9082 is reactive to GFAP in Human, Mouse, Rat

Applications

PB9082 is guaranteed for IF, IHC, WB Boster Guarantee

Observed Molecular Weight

50 kDa

Calculated molecular weight

49.88kDa

Background of GFAP

Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, and ependymal cells. It is mapped to 17q21.31. GFAP is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells. This gene has been shown to play a role in mitosis by adjusting the filament network present in the cell. GFAP is necessary for many critical roles in the CNS. What’s more, GFAP also plays a role in astrocyte-neuron interactions as well as cell-cell communication.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, Pig, By Heat
Immunofluorescence, 5μg/ml, Rat

Validation Images & Assay Conditions

Gene/Protein Information For GFAP (Source: Uniprot.org, NCBI)

Gene Name

GFAP

Full Name

Glial fibrillary acidic protein

Weight

49.88kDa

Superfamily

intermediate filament family

Alternative Names

FLJ45472; GFAP astrocytes; GFAP immunohistochemistry; GFAP mouse; GFAP rabbit; GFAP stain; GFAP; glial fibrillary acidic protein GFAP ALXDRD glial fibrillary acidic protein glial fibrillary acidic protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GFAP, check out the GFAP Infographic

GFAP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GFAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PB9082 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model

Effect of electroacupuncture on glial fibrillary acidic protein and nerve growth factor in the hippocampus of rats with hyperlipidemia and middle cerebral artery thrombus

Effect of all-trans retinoic acid on the proliferation and differentiation of brain tumor stem cells

Electroacupuncture alleviates spatial memory deficits in METH withdrawal mice by enhancing astrocyte-mediated glutamate clearance in the dCA1

Klotho ameliorated cognitive deficits in a temporal lobe epilepsy rat model by inhibiting ferroptosis

Differentiation of Mesenchymal Stem Cells into Neural Stem Cells Using Cerebrospinal Fluid

Astrocyte-selective STAT3 knockdown rescues methamphetamine withdrawal-disrupted spatial memory in mice via restoring the astrocytic capacity of glutamate clearance in dCA1

Tak1 in the astrocytes of mediobasal hypothalamus regulates anxiety‐like behavior in mice

Maternal Benzophenone Exposure Impairs Hippocampus Development and Cognitive Function in Mouse Offspring

Maternal Benzophenone Exposure Impairs Hippocampus Development and Cognitive Function in Mouse Offspring

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16 Customer Q&As for Anti-GFAP Antibody Picoband™

Question

I see that the anti-GFAP antibody PB9082 works with IHC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2020-04-23

Answer

You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2020-04-23

Question

I have attached the WB image, lot number and protocol we used for fetal brain using anti-GFAP antibody PB9082. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2020-02-28

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2020-02-28

Question

Is this PB9082 anti-GFAP antibody reactive to the isotypes of GFAP?

Verified Customer

Verified customer

Asked: 2020-02-04

Answer

The immunogen of PB9082 anti-GFAP antibody is E.coli-derived human GFAP recombinant protein (Position: Q93-M432). Human GFAP shares 94% amino acid (aa) sequence identity with both mouse and rat GFAP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2020-02-04

Question

My team were well pleased with the WB result of your anti-GFAP antibody. However we have observed positive staining in kidney cytoplasm. using this antibody. Is that expected? Could you tell me where is GFAP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2020-01-06

Answer

Based on literature, kidney does express GFAP. Generally GFAP expresses in cytoplasm. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2020-01-06

Question

We are currently using anti-GFAP antibody PB9082 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-12-18

Answer

The anti-GFAP antibody (PB9082) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-12-18

Question

We have seen staining in mouse fetal brain. What should we do? Is anti-GFAP antibody supposed to stain fetal brain positively?

Verified Customer

Verified customer

Asked: 2019-10-29

Answer

From literature fetal brain does express GFAP. From Uniprot.org, GFAP is expressed in dorsal motor nucleus of vagus nerve, brain thalamus, brain, kidney, fetal brain cortex, fetal brain, blood, among other tissues. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005

Boster Scientific Support

Answered: 2019-10-29

Question

Do you have a BSA free version of anti-GFAP antibody PB9082 available?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GFAP antibody PB9082 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2019-09-19

Question

Will PB9082 anti-GFAP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2019-09-13

Answer

As indicated on the product datasheet, PB9082 anti-GFAP antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2019-09-13

Question

Will anti-GFAP antibody PB9082 work for IHC with fetal brain?

Verified Customer

Verified customer

Asked: 2019-08-08

Answer

According to the expression profile of fetal brain, GFAP is highly expressed in fetal brain. So, it is likely that anti-GFAP antibody PB9082 will work for IHC with fetal brain.

Boster Scientific Support

Answered: 2019-08-08

Question

Is a blocking peptide available for product anti-GFAP antibody (PB9082)?

Verified Customer

Verified customer

Asked: 2019-07-15

Answer

We do provide the blocking peptide for product anti-GFAP antibody (PB9082). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-07-15

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-GFAP antibody PB9082. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-06

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-06

Question

My question regarding product PB9082, anti-GFAP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-05-23

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9082 anti-GFAP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-05-23

Question

I was wanting to use your anti-GFAP antibody for IHC for rat fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat fetal brain identification?

Verified Customer

Verified customer

Asked: 2019-04-18

Answer

As indicated on the product datasheet, PB9082 anti-GFAP antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-04-18

Question

We ordered your anti-GFAP antibody for WB on dorsal motor nucleus of vagus nerve a few months ago. I am using rat, and I plan to use the antibody for IHC next. I was wanting to use examining dorsal motor nucleus of vagus nerve as well as kidney in our next experiment. Could give a recommendation on which antibody would work the best for IHC?

Verified Customer

Verified customer

Asked: 2018-07-26

Answer

I looked at the website and datasheets of our anti-GFAP antibody and I see that PB9082 has been tested on rat in both WB and IHC. Thus PB9082 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2018-07-26

Question

My question regards to test anti-GFAP antibody PB9082 on rat fetal brain for research purposes, then I may be interested in using anti-GFAP antibody PB9082 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

S. Mitchell

Verified customer

Asked: 2017-04-19

Answer

The products we sell, including anti-GFAP antibody PB9082, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2017-04-19

Question

We need using your anti-GFAP antibody for chaperone mediated autophagy studies. Has this antibody been tested with western blotting on neuro whole cell lysate? We would like to see some validation images before ordering.

K. Brown

Verified customer

Asked: 2013-05-10

Answer

Thank you for your inquiry. This PB9082 anti-GFAP antibody is tested on mouse brain, brain tissue, tissue lysate, rat brain tissue, human meningioma tissue, u87 whole cell lysate, shg whole cell lysate, neuro whole cell lysate, hela whole cell lysate. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2013-05-10

Order DetailsPrice
PB9082

100μg

$370
PB9082-10ug

10μg sample (liquid)

$99
PB9082-Biotin

100 μg Biotin conjugated

$570
PB9082-Cy3

100 μg Cy3 conjugated

$570
PB9082-Dylight488

100 μg Dylight488 conjugated

$570
PB9082-Dylight550

100 μg Dylight550 conjugated

$570
PB9082-Dylight594

100 μg Dylight594 conjugated

$570
PB9082-FITC

100 μg FITC conjugated

$570
PB9082-HRP

100 μg HRP conjugated

$570
PB9082-APC

100 μg APC conjugated

$670
PB9082-PE

100 μg PE conjugated

$670
PB9082-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
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