Anti-Zinc finger protein GLI2 Gli2 Antibody

GLI-2 antibody

Boster Bio Anti-Zinc finger protein GLI2 Gli2 Antibody catalog # PA1941. Tested in WB applications. This antibody reacts with Human. Cited in 2 publication(s).

Product Info Summary

SKU: PA1941
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: WB

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Product Name

Anti-Zinc finger protein GLI2 Gli2 Antibody

View all GLI-2 Antibodies

SKU/Catalog Number

PA1941

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Zinc finger protein GLI2 Gli2 Antibody catalog # PA1941. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Zinc finger protein GLI2 Gli2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1941)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Gli2.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1941 is reactive to GLI2 in Human

Applications

PA1941 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

89 kDa

Calculated molecular weight

167.783kDa

Background of GLI-2

GLI2 (Gli-Kruppel Family Member 2), also called ONCOGENE GLI2, is a protein that in humans is encoded by the GLI2 gene. Sequencing of GLI cDNA clones showed the presence of 5 tandem zinc fingers connected by histidine-cysteine links, which indicated that the gene belongs to the family of zinc finger genes related to Kruppel (Kr). The Drosophila gene Kr is a member of the gap class of segmentation genes; thoracic and anterior abdominal segments fail to form in Kr mutant embryos. By fluorescence in situ hybridization, Matsumoto et al. (1996) refined the assignment of the GLI2 gene to chromosome 2q14. Roessler et al. (2005) showed that GLI2-delta-N exhibited potent transcriptional activity in vivo: overexpression in mouse skin led to the formation of hedgehog-independent epithelial downgrowths resembling basal cell carcinomas, which in humans are associated with constitutive hedgehog signaling.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Innovating Scientists Reward

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Gene/Protein Information For GLI2 (Source: Uniprot.org, NCBI)

Gene Name

GLI2

Full Name

Zinc finger protein GLI2

Weight

167.783kDa

Superfamily

GLI C2H2-type zinc-finger protein family

Alternative Names

GLI family zinc finger 2; GLI2; GLI-2; GLI-Kruppel family member GLI2; glioma-associated oncogene family zinc finger 2; HPE9tax helper protein 1; oncogene GLI2; tax helper protein 2; Tax helper protein; tax-responsive element-2 holding protein; tax-responsive element-25-bp sequence binding protein; THP; THP1; THP2; zinc finger protein GLI2 GLI2 CJS, HPE9, PHS2, THP1, THP2 GLI family zinc finger 2 zinc finger protein GLI2|GLI family zinc finger protein 2|GLI-Kruppel family member GLI2|glioma-associated oncogene family zinc finger 2|oncogene GLI2|tax helper protein 1|tax helper protein 2|tax-responsive element-2 holding protein|tax-responsive element-25-bp sequence binding protein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GLI2, check out the GLI2 Infographic

GLI2 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GLI2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

PA1941 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

O-GlcNAcylation of GLI transcription factors in hyperglycemic conditions augments Hedgehog activity

Xiang W, Jiang T, Guo F, Gong C, Yang K, Wu Y, Huang X, Cheng W, Xu K. Oncol Rep. 2014 Oct;32(4):1622-30. Doi: 10.3892/Or.2014.3372. Epub 2014 Jul 31. Hedgehog Pathway Inhibitor-4 Suppresses Malignant Properties Of Chondrosarcoma Cells By Disturbi...

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5 Customer Q&As for Anti-Zinc finger protein GLI2 Gli2 Antibody

Question

Is there a BSA free version of anti-Gli2 antibody PA1941 available?

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Gli2 antibody PA1941 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-01-09

Question

We are currently using anti-Gli2 antibody PA1941 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on pig tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

The anti-Gli2 antibody (PA1941) has not been tested for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-12

Question

Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for cervix carcinoma using anti-Gli2 antibody PA1941. Let me know if you need anything else.

Verified Customer

Verified customer

Asked: 2019-06-27

Answer

Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-06-27

Question

Would anti-Gli2 antibody PA1941 work on monkey WB with cervix carcinoma?

Verified Customer

Verified customer

Asked: 2019-06-19

Answer

Our lab technicians have not validated anti-Gli2 antibody PA1941 on monkey. You can run a BLAST between monkey and the immunogen sequence of anti-Gli2 antibody PA1941 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated monkey samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in monkey cervix carcinoma in WB, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-06-19

Question

Is a blocking peptide available for product anti-Gli2 antibody (PA1941)?

Verified Customer

Verified customer

Asked: 2019-04-05

Answer

We do provide the blocking peptide for product anti-Gli2 antibody (PA1941). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-04-05

Order DetailsPrice
PA1941

100μg

$370
PA1941-10ug

10μg sample (liquid)

$99
PA1941-Biotin

100 μg Biotin conjugated

$570
PA1941-Cy3

100 μg Cy3 conjugated

$570
PA1941-Dylight488

100 μg Dylight488 conjugated

$570
PA1941-Dylight550

100 μg Dylight550 conjugated

$570
PA1941-Dylight594

100 μg Dylight594 conjugated

$570
PA1941-FITC

100 μg FITC conjugated

$570
PA1941-HRP

100 μg HRP conjugated

$570
PA1941-APC

100 μg APC conjugated

$670
PA1941-PE

100 μg PE conjugated

$670
PA1941-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

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PA1941
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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