Data & Images
|Product Name||Anti-Gli2 Antibody|
|Description||Rabbit IgG polyclonal antibody for Zinc finger protein GLI2(GLI2) detection. Tested with WB in Human.|
|Cite This Product||Anti-Gli2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1941)|
|Replacement Item||This antibody may replace the following items: sc-20290|sc-20290-X|sc-20291|sc-20291-X|sc-271786|sc-271786-X|sc-28674|sc-28674-X from Santa Cruz Biotechnology.|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||A synthetic peptide corresponding to a sequence at the C-terminus of human Gli2(1444-1463aa MLYYYGQIHMYEQDGGLENL).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Zinc finger protein GLI2|
|Molecular Weight||167783 MW|
|Protein Function||Has a dual function as a transcriptional activator and a repressor of the hedgehog (Hh) pathway. May play a role during embryogenesis. .|
|Tissue Specificity||Isoform 1 and isoform 4 are expressed in HTLV- 1-infected T-cell lines (at protein level). Isoform 1 and isoform 2 are strongly expressed in HTLV-1-infected T-cell lines. Isoform 3 and isoform 4 are weakly expressed in HTLV-1-infected T-cell lines. .|
|Sequence Similarities||Belongs to the GLI C2H2-type zinc-finger protein family.|
|Subcellular Localization||Nucleus . Cytoplasm . In keratinocytes, it is sequestered in the cytoplasm by SUFU. In the absence of SUFU, it translocates to the nucleus. .|
|Alternative Names||Zinc finger protein GLI2 ;GLI family zinc finger protein 2 ;Tax helper protein ;GLI2 ;THP ;|
|Research Areas|||epigenetics and nuclear signaling|transcription|domain families|zinc finger| neuroscience|neurology process|neural signal transduction| stem cells|signaling pathways|hedgehog|nuclear| epigenetics and nuclear signaling|chip'ing antibodies||
Background for Zinc finger protein GLI2
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Gli2 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Western blot, 0.1-0.5μg/ml, Human|
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Gli2 Antibody Images
Click the images to enlarge.
WB: Human Placenta Tissue Lysate
Lane 1: MCF-7 Cell Lysate
Lane 2: HELA Cell Lysate
Lane 3: SKOV Cell Lysate
Lane 4: HT1080 Cell Lysate
Lane 5: A549 Cell Lysate
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,