|Application:||IHC-P, IHC-F, WB|
Data & Images
|Product Name||Anti-GLUT4 Picoband™ Antibody|
|Description||Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 4(SLC2A4) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.|
|Cite This Product||Anti-GLUT4 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9109)|
|Replacement Item||This antibody may replace the following items: sc-130464|sc-1606|sc-1608|sc-17558|sc-17558-R|sc-53566|sc-7938|sc-85920|sc-85920-X|sc-85921|sc-85921-X from Santa Cruz Biotechnology.|
|Validated Species||Human, Mouse, Rat|
|Application||IHC-P, IHC-F, WB
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
|Immunogen||E.coli-derived human GLUT4 recombinant protein (Position: N333-D509). Human GLUT4 shares 97% amino acid (aa) sequence identity with mouse GLUT4.|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Solute carrier family 2, facilitated glucose transporter member 4|
|Molecular Weight||54787 MW|
|Protein Function||Insulin-regulated facilitative glucose transporter.|
|Tissue Specificity||Skeletal and cardiac muscles; brown and white fat.|
|Sequence Similarities||Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.|
|Subcellular Localization||Cell membrane ; Multi-pass membrane protein . Endomembrane system ; Multi-pass membrane protein . Cytoplasm, perinuclear region . Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.|
|Alternative Names||Solute carrier family 2, facilitated glucose transporter member 4;Glucose transporter type 4, insulin-responsive;GLUT-4;SLC2A4;GLUT4;|
|Research Areas|||signal transduction|metabolism|energy metabolism| epigenetics and nuclear signaling|transcription|other factors|transcription factors| cardiovascular|heart|cardiac metabolism| metabolism|pathways and processes|metabolic signaling pathways|energy transfer pathways||
Background for Solute carrier family 2, facilitated glucose transporter member 4
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-GLUT4 Picoband™ Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Frozen Section), 0.5-1μg/ml, Mouse, Rat, -|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Rat, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-GLUT4 Picoband™ Antibody Images
Click the images to enlarge.
All lanes: Anti GLUT4 (PB9109) at 0.5ug/ml
Lane 1: Rat Cardiac MuscleTissue Lysate at 50ug
Lane 2: Rat Skeletal MuscleTissue Lysate at 50ug
Predicted bind size: 55KD
Observed bind size: 55KD
IHC(F): Rat Cardiac Muscle Tissue
IHC(F): Mouse Cardiac Muscle Tissue
IHC(P): Human Intestinal Cancer Tissue
IHC(P): Rat Cardiac Muscle Tissue
IHC(P): Mouse Skeletal Muscle Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,