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SKU:PB9109
Clonality:Polyclonal
Application:IHC-P, IHC-F, WB
Price: $240.00
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Overview

Product Name Anti-GLUT4 Picoband™ Antibody
Description Rabbit IgG polyclonal antibody for Solute carrier family 2, facilitated glucose transporter member 4(SLC2A4) detection. Tested with WB, IHC-P, IHC-F in Human;Mouse;Rat.
Cite This Product Anti-GLUT4 Picoband™ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9109)
Replacement Item This antibody may replace the following items: sc-130464|sc-1606|sc-1608|sc-17558|sc-17558-R|sc-53566|sc-7938|sc-85920|sc-85920-X|sc-85921|sc-85921-X from Santa Cruz Biotechnology.
Host Rabbit
Isotype N/A
Validated Species Human, Mouse, Rat
Application IHC-P, IHC-F, WB

*Our Boster Guarantee covers the use of this product in the above tested applications.

**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.

Recommended Detection Systems Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P) and IHC(F).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2017!
Immunogen E.coli-derived human GLUT4 recombinant protein (Position: N333-D509). Human GLUT4 shares 97% amino acid (aa) sequence identity with mouse GLUT4.
Cross Reactivity No cross reactivity with other proteins
Pack Size 100μg/vial

Properties

Clonality Polyclonal
Form Lyophilized
Contents Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
*carrier free antibody available upon request.
Concentration Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Storage At -20˚C for one year. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for a longer time.Avoid repeated freezing and thawing.
Purification Immunogen affinity purified.
Isotype N/A

Protein Target Info (Source: Uniprot.org)

You can check the tissue specificity below for information on selecting positive and negative control.

Gene Name SLC2A4
Protein Name Solute carrier family 2, facilitated glucose transporter member 4
Molecular Weight 54787 MW
Protein Function Insulin-regulated facilitative glucose transporter.
Tissue Specificity Skeletal and cardiac muscles; brown and white fat.
Sequence Similarities Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family. Glucose transporter subfamily.
Subcellular Localization Cell membrane ; Multi-pass membrane protein . Endomembrane system ; Multi-pass membrane protein . Cytoplasm, perinuclear region . Localizes primarily to the perinuclear region, undergoing continued recycling to the plasma membrane where it is rapidly reinternalized. The dileucine internalization motif is critical for intracellular sequestration.
Uniprot ID P14672
Alternative Names Solute carrier family 2, facilitated glucose transporter member 4;Glucose transporter type 4, insulin-responsive;GLUT-4;SLC2A4;GLUT4;
Research Areas |signal transduction|metabolism|energy metabolism| epigenetics and nuclear signaling|transcription|other factors|transcription factors| cardiovascular|heart|cardiac metabolism| metabolism|pathways and processes|metabolic signaling pathways|energy transfer pathways|
*if product is indicated to react with multiple species, protein info is based on the human gene.

Background for Solute carrier family 2, facilitated glucose transporter member 4

GLUT4, also known as SLC2A4 or solute carrier family 2 (facilitated glucose transporter) member 4, is a protein that in humans is encoded by the GLUT4 gene. It is mapped to 17p13.1. This gene is a member of the solute carrier family 2 (facilitated glucose transporter) family and encodes a protein that functions as an insulin-regulated facilitative glucose transporter. In the absence of insulin, this integral membrane protein is sequestered within the cells of muscle and adipose tissue. Within minutes of insulin stimulation, the protein moves to the cell surface and begins to transport glucose across the cell membrane. Mutations in this gene have been associated with noninsulin-dependent diabetes mellitus (NIDDM).

Anti-GLUT4 Picoband™ Antibody Images

Click the images to enlarge.

Anti-GLUT4 Picoband™ Antibody
Anti-GLUT4 Picoband antibody, PB9109-1.jpg
All lanes: Anti GLUT4 (PB9109) at 0.5ug/ml
Lane 1: Rat Cardiac MuscleTissue Lysate at 50ug
Lane 2: Rat Skeletal MuscleTissue Lysate at 50ug
Predicted bind size: 55KD
Observed bind size: 55KD
Anti-GLUT4 Picoband™ Antibody
Anti-GLUT4 Picoband antibody, PB9109-2.JPG
IHC(F): Rat Cardiac Muscle Tissue
Anti-GLUT4 Picoband™ Antibody
Anti-GLUT4 Picoband antibody, PB9109-3.JPG
IHC(F): Mouse Cardiac Muscle Tissue
Anti-GLUT4 Picoband™ Antibody
Anti-GLUT4 Picoband antibody, PB9109-4.JPG
IHC(P): Human Intestinal Cancer Tissue
Anti-GLUT4 Picoband™ Antibody
Anti-GLUT4 Picoband antibody, PB9109-5.JPG
IHC(P): Rat Cardiac Muscle Tissue
Anti-GLUT4 Picoband™ Antibody
Anti-GLUT4 Picoband antibody, PB9109-6.JPG
IHC(P): Mouse Skeletal Muscle Tissue
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Publications

Zhou X, Wu W, Chen J, Wang X, Wang Y. Nutr Metab (Lond). 2015 Mar 8;12:10. Doi: 10.1186/S12986-015-0006-5. Ecollection 2015. Amp-Activated Protein Kinase Is Required For The Anti-Adipogenic Effects Of Alpha-Linolenic Acid.

FAQs

Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
Q: What should I use for negative control?
A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
A: You can find the immunogen sequence under "Immunogen" and clonality in the product name.
Q: What is the expected band size? Why is it different than the observed band size?
A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands

3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.

4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
A: Check our protocols under the tech support tab.
Q: What are some alternative names that could be used to describe this product?
A: Some common names include but are not limited to glut4 antibody, glut 4 antibody, glut-4 antibody