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Product Info Summary
|Application:||Flow Cytometry, WB|
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Anti-Glypican 3 Monoclonal Antibody
Boster Bio Anti-Glypican 3 Monoclonal Antibody catalog # M01922-1. Tested in Flow Cytometry, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Glypican 3 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01922-1)
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
A synthesized peptide derived from human Glypican 3 Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
M01922-1 is reactive to GPC3 in Human
M01922-1 is guaranteed for Flow Cytometry, WB Boster Guarantee
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
WB 1:500~1:2000 FC 1:50
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of GPC3 expression in HepG2 cell lysate using anti-GPC3 antibody (M01922-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GPC3 antigen affinity purified polyclonal antibody (Catalog # M01922-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for GPC3.
Gene/Protein Information For GPC3 (Source: Uniprot.org, NCBI)
DGSX; Glypican 3; glypican proteoglycan 3; glypican-3; GPC3; GTR2-2; heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI5; OCI-5; secreted glypican-3; SGB; SGBS; SGBS1SDYS GPC3 DGSX, GTR2-2, MXR7, OCI-5, SDYS, SGB, SGBS, SGBS1 glypican 3 glypican-3|glypican proteoglycan 3|heparan sulphate proteoglycan|intestinal protein OCI-5|secreted glypican-3*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on GPC3, check out the GPC3 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GPC3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected]
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