Anti-Gremlin 1/GREM1 Antibody Picoband™

Gremlin 1 antibody

Boster Bio Anti-Gremlin 1/GREM1 Antibody Picoband™ catalog # PB9626. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9626
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

Product Name

Anti-Gremlin 1/GREM1 Antibody Picoband™

View all Gremlin 1 Antibodies

SKU/Catalog Number

PB9626

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Gremlin 1/GREM1 Antibody Picoband™ catalog # PB9626. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Gremlin 1/GREM1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9626)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of human Gremlin 1, identical to the related mouse and rat sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9626 is reactive to Grem1 in Human, Mouse, Rat

Applications

PB9626 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

21 kDa

Calculated molecular weight

20.71kDa

Background of Gremlin 1

Gremlin, also known as Drm, is a highly conserved 20.7-kDa, 184 amino acid glycoprotein part of the DAN family and is a cysteine knot-secreted protein. Skeletal cells synthesize bone morphogenetic proteins (BMPs) and BMP antagonists. And Gremlin is expressed in osteoblasts and opposes BMP effects on osteoblastic differentiation and function in vitro. Gremlin 1 (GREM 1) is known for its antagonistic reaction with BMPs in the TGF beta signaling pathway. This gene inhibits BMP-2, BMP-4, and BMP-7. Inhibition by grem 1 of BMPs in mice allow the expression of fibroblast growth factors (FGFs) 4 and 8 and Sonic hedgehog (SHH) which are necessary for proper limb development. Gremlin 1 may play an oncogenic role especially in carcinomas of the uterine cervix, lung, ovary, kidney, breast, colon, pancreas, and sarcoma. Over-expressed gremlin 1 functions by interaction with YWHAH (Its binding site for gremlin 1 was located between residues 61-80 and gremlin 1 binding site for YWHAH was found to be located between residues 1-67). Therefore, Gremlin 1 and its binding protein YWHAH could be good targets for developing diagnostic and therapeutic strategies against human cancers.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.

Submit A Review

Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For Grem1 (Source: Uniprot.org, NCBI)

Gene Name

Grem1

Full Name

Gremlin-1

Weight

20.71kDa

Superfamily

DAN family

Alternative Names

Cell proliferation-inducing gene 2 protein; CKTSF1B1gremlin 1, cysteine knot superfamily, homolog; Cysteine knot superfamily 1, BMP antagonist 1gremlin 1, cysteine knot superfamily, homolog (Xenopus laevis); DAN domain family member 2; DAND2; DAND2GREMLIN; Down-regulated in Mos-transformed cells protein; DRM; DRMMGC126660; GREM1; gremlin 1; gremlin 1-like protein; Gremlin; gremlin-1; IHG-2; Increased in high glucose protein 2; increased in high glucose-2; proliferation-inducing gene 2 Grem1|Cktsf1b, Cktsf1b1, Dr, Drm, Grem, gre, ld|gremlin 1, DAN family BMP antagonist|gremlin-1|cysteine knot superfamily 1, BMP antagonist 1|down-regulated in Mos-transformed cells protein|down-regulated in v-mos-transformed cells

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on Grem1, check out the Grem1 Infographic

Grem1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for Grem1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

No publications found for PB9626

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Anti-Gremlin 1/GREM1 Antibody Picoband™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Anti-Gremlin 1/GREM1 Antibody Picoband™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

3 Customer Q&As for Anti-Gremlin 1/GREM1 Antibody Picoband™

Question

We are currently using anti-Gremlin 1/GREM1 antibody PB9626 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, rat. Is it likely that the antibody can work on dog tissues as well?

Verified Customer

Verified customer

Asked: 2019-02-11

Answer

The anti-Gremlin 1/GREM1 antibody (PB9626) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-02-11

Question

I was wanting to use your anti-Gremlin 1/GREM1 antibody for WB for rat liver on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat liver identification?

Verified Customer

Verified customer

Asked: 2018-07-12

Answer

As indicated on the product datasheet, PB9626 anti-Gremlin 1/GREM1 antibody has been validated for WB on human, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat liver in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-07-12

Question

See attached the WB image, lot number and protocol we used for liver using anti-Gremlin 1/GREM1 antibody PB9626. Please let me know if you require anything else.

P. Zhao

Verified customer

Asked: 2015-07-31

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-07-31

Order DetailsPrice
PB9626

100μg

$370
PB9626-10ug

10μg sample (liquid)

$99
PB9626-Biotin

100 μg Biotin conjugated

$570
PB9626-Cy3

100 μg Cy3 conjugated

$570
PB9626-Dylight488

100 μg Dylight488 conjugated

$570
PB9626-Dylight550

100 μg Dylight550 conjugated

$570
PB9626-Dylight594

100 μg Dylight594 conjugated

$570
PB9626-FITC

100 μg FITC conjugated

$570
PB9626-HRP

100 μg HRP conjugated

$570
PB9626-APC

100 μg APC conjugated

$670
PB9626-PE

100 μg PE conjugated

$670
PB9626-carrier-free

Carrier Free

$370

More conjugates/formats

Free Secondary Antibody

Choose an option to show lead time.
Get A Quote
Eddy test
In stock
Order Product
PB9626
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.