Anti-Glutamate Receptor 1/GRIA1 Antibody


SKU PA1587
Size 100μg/vial
Reactivity Human, Mouse, Rat
Clonality Polyclonal
Host Rabbit
Ig Isotype N/A
Applications WB

Overview

Product Name Anti-Glutamate Receptor 1/GRIA1 Antibody
SKU/Catalog Number PA1587
Storage & Handling At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.
Size 100μg/vial
Description Rabbit IgG polyclonal antibody for Glutamate receptor 1(GRIA1) detection. Tested with WB in Human;Mouse;Rat.
Cite This Product Anti-Glutamate Receptor 1/GRIA1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1587)
Host Rabbit
Contents/Buffer Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Form Lyophilized
Immunogen A synthetic peptide corresponding to a sequence at the C-terminus of human GRIA1 (881-895aa HDFPKSMQSIPCMSH), different from the related rat and mouse sequences by one amino acid.
Reactivity Human, Mouse, Rat

Assay Details

Assay Dilutions Overview

Concentration: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Western blot, 0.1-0.5μg/ml, Rat, Human, Mouse

Boster's Secondary Antibodies And IHC, WB Kits

The following reagents are used to generate the images below.

Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.

Images And Assay Conditions

Anti- GRIA1 antibody, PA1587, Western blotting
All lanes: Anti GRIA1 (PA1587) at 0.5ug/ml
WB: Rat Brain Tissue Lysate at 50ug
Predicted bind size: 101KD
Observed bind size: 101KD

Target Info

Protein Target Info (Source: Uniprot.org)

Uniprot Id P42261
Gene Name GRIA1
Protein Name Glutamate receptor 1
Tissue Specificity Widely expressed in brain.
Alternative Names Glutamate receptor 1;GluR-1;AMPA-selective glutamate receptor 1;GluR-A;GluR-K1;Glutamate receptor ionotropic, AMPA 1;GluA1;GRIA1;GLUH1, GLUR1;
Subcellular Localization Cell membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell projection, dendrite . Cell projection, dendritic spine . Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression. .
Molecular Weight 101506 MW

*if product is indicated to react with multiple species, protein info is based on the human gene.

Ontology

Protein Function Ionotropic glutamate receptor. L-glutamate acts as an excitatory neurotransmitter at many synapses in the central nervous system. Binding of the excitatory neurotransmitter L- glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist. In the presence of CACNG4 or CACNG7 or CACNG8, shows resensitization which is characterized by a delayed accumulation of current flux upon continued application of glutamate. .
Research Areas Human, Mouse, Rat

*You can search these to find other products in these research areas.
Background GLUR1, Glutamate receptor 1, is a protein that in humans is encoded by the GLUR1 gene. The sequence of GLUR1 was predicted to encode a 907-amino acid protein that had 97% identity to one of the rodent kainate receptor subunits. GLUR1 mRNA is widely expressed in human brain. Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes with multiple subunits, each possessing transmembrane regions, and all arranged to form a ligand-gated ion channel. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The GRIA1 belongs to a family of alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate(AMPA) receptors. Each of the members(GRIA1-4) include flip and flop isoforms generated by alternative RNA splicing. The receptor subunits encoded by each isoform vary in their signal transduction properties. The isoform presented here is the flop isoform. In situ hybridization experiments showed that human GRIA1 mRNA is present in granule and pyramidal cells in the hippocampal formation.

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Polyclonal antibody for GluR1/GRIA1 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: WB. Reactive species: Human. GluR1/GRIA1 information: Molecular Weight: 101506 MW; Subcellular Localization: Cell membrane ; Multi-pass membrane protein . Endoplasmic reticulum membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane ; Multi-pass membrane protein . Cell junction, synapse, postsynaptic cell membrane, postsynaptic density . Cell projection, dendrite . Cell projection, dendritic spine . Interaction with CACNG2, CNIH2 and CNIH3 promotes cell surface expression; Tissue Specificity: Widely expressed in brain.
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In stock
Order Product
PA1587
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

Troubleshooting

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Publications

Loss of the golgin GM130 causes Golgi disruption, Purkinje neuron loss, and ataxia in mice
Zhong C, Han Y, Qiu J, Lu L, Chen Y, Chen J, Hei R, Mi W. Int J Pediatr Otorhinolaryngol. 2010 Feb;74(2):192-7. Doi: 10.1016/J.Ijporl.2009.11.009. Epub 2009 Dec 5. A Comparison Of The Proliferative Capacity And Ultrastructure Of Proliferative Cell...

Customer Q&As

  • Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugation
    A: Yes, please contact us at support@bosterbio.com for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
  • Q: Is your western blot protocol provided from the website applicable for all your antibodies? Keyword: applications, WB
    A: The protocol is applicable for all our antibodies in WB, the NC Membrane(0.45μm or 0.22μm) and transfer time(70 mins or 50 mins) depends on the protein molecular weight, details can be found in included protocol.
  • Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugation
    A: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact support@bosterbio.com
  • Q: What should I use for negative control?
    A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
  • Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signal
    A: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
  • Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?
    A: You can find the immunogen sequence under "
  • Q: What is the expected band size? Why is it different than the observed band size?
    A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
  • Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?
    A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
  • Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?
    A: Check our protocols under the tech support tab.
  • Q: What are some alternative names that could be used to describe this product?
    A: Some common names include but are not limited to glur1 antibody, glua1 antibody, glutamate receptor 1 antibody
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