Anti-GRM5/Mglur5 Rabbit Monoclonal Antibody

GRM5 antibody

Boster Bio Anti-GRM5/Mglur5 Rabbit Monoclonal Antibody catalog # M01338. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: M01338
Size: 100 μl
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, IF, IHC, ICC, WB

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Product Name

Anti-GRM5/Mglur5 Rabbit Monoclonal Antibody

View all GRM5 Antibodies

SKU/Catalog Number

M01338

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-GRM5/Mglur5 Rabbit Monoclonal Antibody catalog # M01338. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-GRM5/Mglur5 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01338)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

EBH-7

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human GRM5

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

Detects ~20kDa. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, HSP25, HSP27 or HSP47 proteins.

Reactive Species

M01338 is reactive to GRM5 in Human, Mouse, Rat

Applications

M01338 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

17 kDa

Calculated molecular weight

132.469kDa

Background of GRM5

Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH. Involved in menthol sensation. Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200

Validation Images & Assay Conditions

Gene/Protein Information For GRM5 (Source: Uniprot.org, NCBI)

Gene Name

GRM5

Full Name

Metabotropic glutamate receptor 5

Weight

132.469kDa

Superfamily

G-protein coupled receptor 3 family

Alternative Names

glutamate receptor, metabotropic 5; GPRC1EMGLUR5metabotropic glutamate receptor 5; mGlu5; mGluR5 GRM5 GPRC1E, MGLUR5, PPP1R86, mGlu5 glutamate metabotropic receptor 5 metabotropic glutamate receptor 5|glutamate receptor, metabotropic 5|protein phosphatase 1, regulatory subunit 86

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on GRM5, check out the GRM5 Infographic

GRM5 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for GRM5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-GRM5/Mglur5 Rabbit Monoclonal Antibody

Question

I am looking for to test anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 on rat brain for research purposes, then I may be interested in using anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

Verified Customer

Verified customer

Asked: 2019-10-21

Answer

The products we sell, including anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2019-10-21

Question

Does anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 work for IF with brain?

Verified Customer

Verified customer

Asked: 2019-05-17

Answer

According to the expression profile of brain, GRM5 is highly expressed in brain. So, it is likely that anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 will work for IF with brain.

Boster Scientific Support

Answered: 2019-05-17

Question

Does anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 work on canine IF with brain?

Verified Customer

Verified customer

Asked: 2018-06-06

Answer

Our lab technicians have not tested anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 on canine. You can run a BLAST between canine and the immunogen sequence of anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain in IF, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-06-06

Question

Can you help my question with product M01338, anti-GRM5/Mglur5 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

V. Roberts

Verified customer

Asked: 2016-10-28

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01338 anti-GRM5/Mglur5 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2016-10-28

Question

Is there a BSA free version of anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 available?

F. Kulkarni

Verified customer

Asked: 2016-10-17

Answer

We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2016-10-17

Question

Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-GRM5/Mglur5 Rabbit Monoclonal antibody M01338. Let me know if you need anything else.

S. Anderson

Verified customer

Asked: 2016-01-26

Answer

I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2016-01-26

Question

Is this M01338 anti-GRM5/Mglur5 Rabbit Monoclonal antibody reactive to the isotypes of GRM5?

T. Moore

Verified customer

Asked: 2015-05-14

Answer

The immunogen of M01338 anti-GRM5/Mglur5 Rabbit Monoclonal antibody is A synthesized peptide derived from human GRM5. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2015-05-14

Order DetailsPrice
M01338

100μL

$370
M01338-30ul

30μL sample

$99
M01338-carrier-free

Carrier Free

$370

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Free Secondary Antibody

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M01338
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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