Anti-GRO gamma/CXCL3 Antibody Picoband™
Rabbit IgG polyclonal antibody for C-X-C motif chemokine 3(CXCL3) detection. Tested with WB, ELISA in Mouse.
|Product Name||Anti-GRO gamma/CXCL3 Antibody Picoband™
See all CXCL3 primary antibodies, ELISA kits and proteins
|Storage & Handling||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Description||Polyclonal antibody for Cxcl3 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: ELISA. Reactive species: Mouse. Cxcl3 information: Molecular Weight: 10686 MW; Subcellular Localization: Secreted .|
|Cite This Product||Anti-GRO gamma/CXCL3 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9941)|
|Specificity||Anti-GRO gamma/CXCL3 Antibody Picoband™ (PB9941) reacts with Mouse CXCL3, in native form and recombinant. Superfamily members of CXCL3 are not reactive to PB9941.|
|Contents/Buffer||Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.|
|Reconstitution||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Immunogen||E. coli-derived mouse GRO gamma recombinant protein (Position: S32-S100). Mouse GRO gamma shares 60.6% and 90.6% amino acid (aa) sequence identity with human and rat GRO gamma, respectively.|
Our Boster Quality Guarantee for Anti-GRO gamma/CXCL3 Antibody Picoband™ covers its use in the following applications.
*The recommended dilution ratios/concentrations are for reference only and optimal dilutions/concentrations should be determined by the end user.
Assay Dilutions Overview
ELISA , 0.1-0.5μg/ml, Mouse, -
Western blot, 0.1-0.5μg/ml, Mouse
Boster's Compatible Products
The following reagents are used to generate the images below for Anti-GRO gamma/CXCL3 Antibody Picoband™ (PB9941).Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot.
Images And Assay Conditions
Western blot analysis of GRO gamma expression in mouse lung extract (lane 1) and mouse spleen extract (lane 2). GRO gamma at 25KD was detected using rabbit anti- GRO gamma Antigen Affinity purified polyclonal antibody (Catalog # PB9941) at 0.5 ??g/mL. The blot was developed using chemiluminescence (ECL) method (Catalog # EK1002).
Protein Target Info (Source: Uniprot.org)
|Protein Name||C-X-C motif chemokine 3|
|Alternative Names||C-X-C motif chemokine 3;Dendritic cell inflammatory protein 1 ;Cxcl3 ;Dcip1 , Gm1960 ;|
|Subcellular Localization||Secreted .|
|Molecular Weight||10686 MW|
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Ligand for CXCR2. Has chemotactic activity for neutrophils. May play a role in inflammation and exert its effects on endothelial cells in an autocrine fashion. .|
*You can search these to find other products in these research areas.
|Background||Chemokine (C-X-C motif) ligand 3 (CXCL3), also known as GRO protein gamma (GROg) and macrophage inflammatory protein-2-beta (MIP2b), is a small cytokine belonging to the CXC chemokine family. It is mapped to 14p2. CXCL3 controls migration and adhesion of monocytes and mediates its effects on its target cell by interacting with a cell surface chemokine receptor. It has been shown that CXCL3 regulates cell autonomously the migration of the precursors of cerebellar granule neurons toward the internal layers of cerebellum, during the morphogenesis of cerebellum. CXCL3 also play fundamental roles in the development, homeostasis and it has effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis.|
Other Recommended Resources
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Publishing with Anti-GRO gamma/CXCL3 Antibody Picoband™ (PB9941)? Please let us know so we can cite the reference in this product datasheet. Email us at [email protected]
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Guaranteed product quality
We promise all of our products perform as described in datasheets.
Q: Do you offer BSA-free antibodies? Keyword: Bovine serum albumin, carrier protein, conjugationA: Yes, please contact us at [email protected] for more information about BSA-free antibodies and availability. The new BSA-free formula uses trehalose as a replacement to BSA. We have tested many alternative chemicals and found that trehalose protects the antibodies the best.
Q: Can I conjugate markers to this antibody? Can I link custom conjugates to this antibody? Keyword: conjugationA: The antibody is stored with BSA and cannot be conjugated with markers. Carrier free antibodies are available upon request. Please contact [email protected]
Q: What should I use for negative control?A: Please contact us for negative control suggestions. You can also check expression databases such as genecards, uniprot etc. Due to logistic reasons, we do not sell serum or lysates that we use internally for positive or negative control.
Q: Where can I find troubleshooting information? What should I do if I have unexpected bands, high background, no signal, weak signalA: You can find Boster's troubleshoot guides under tech support tab. Please contact us for further assistance on troubleshooting your experiment.
Q: What is the immunogen sequence of this antibody? Is this antibody polyclonal or monoclonal?A: You can find the immunogen sequence under "
Q: What is the expected band size? Why is it different than the observed band size?A: The expected band size is predicted on the size of the protein. The actual band size may be affected by a few other factors including but not limited to:<br>1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected<br>2. Post-translational cleavage: this can cause smaller bands and or multiple bands <br><br>3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody. <br><br>4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.<br>5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher., <br>
Q: What is the suggested dilution ratio for Western Blot (WB), Immunohistochemistry (IHC) and or ELISA standards? What is the optimal pH for the sample?A: Check the datasheet for the product for details on dilution ratios for different experiments. You can find the datasheet button on the right side of the product page.
Q: What is the protocol you used for your Western blotting (WB) and Immunohistochemistry (IHC)?A: Check our protocols under the tech support tab.